LOCUS OP_006 7303 bp DNA circular 29-Apr-2025 ACCESSION OP_006 VERSION OP_006.1 FEATURES Location/Qualifiers misc_feature 469..474 /label=BsaI recognition site misc_feature complement(1085..1090) /label=BsaI recognition site misc_feature 1068..1074 /label=SapI recognition site misc_feature complement(485..491) /label=SapI recognition site oriT 5157..5297 /label=basis of mobility region from pBR322 rep_origin 5483..6071 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 2422..3051 /label=pVS1 StaA stability protein CDS 3480..4553 /label=pVS1 RepA replication protein rep_origin 4619..4813 /label=pVS1 oriV origin of replication misc_feature 2183..3183 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 1097..1121 /label=A.tumefaciens plasmid right boundary promoter complement(948..977) /label=lac promoter misc_feature complement(921..941) /label=lac operator CDS complement(582..903) /label=LacZa CDS complement(6159..7169) /label=aadA-1 / Sm resistance rep_origin complement(5468..6082) /label=rep (pMB1) ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acggatcCgg tctcatacta 481 atgtgaagag ccacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 541 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 601 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 661 cgcatcaggc gctcatccgc cattcaggct gcgcaactgt tgggaagggc gatcggcgca 721 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 781 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct 841 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt 901 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 961 gaagcatata gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 1021 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctggct cttcaggtaa 1081 atgtgagaCC GGATcctgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 1141 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 1201 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct 1261 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc atctgaaaac gacatgtcgc 1321 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg 1381 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga 1441 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact 1501 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga 1561 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg 1621 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg 1681 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg 1741 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt 1801 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag 1861 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc 1921 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct 1981 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc 2041 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg 2101 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat 2161 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca 2221 cctctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc 2281 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt 2341 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa 2401 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca 2461 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt 2521 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat 2581 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc 2641 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc 2701 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg 2761 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta 2821 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc 2881 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc 2941 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct 3001 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag 3061 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca 3121 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact 3181 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga 3241 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa 3301 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca 3361 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc 3421 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg 3481 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg 3541 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc 3601 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg 3661 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg 3721 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt 3781 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg 3841 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg 3901 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag 3961 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag 4021 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc 4081 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg 4141 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca 4201 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg 4261 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc 4321 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg 4381 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg 4441 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc 4501 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg 4561 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga ctctttcctg 4621 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg 4681 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga 4741 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc 4801 tggcctgtgc ataactgtct ggccagcgca cagccgaagt gctgcaaaaa gcgcctaccc 4861 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc 4921 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt 4981 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac 5041 ggtgaaaacc tctgacacat gcagctcccg gtgacggtca cagcttgtct gtaagcggat 5101 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 5161 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 5221 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 5281 gaaaataccg catcaggcgc tcatccgctt cctcgctcac tgactcgctg cgctcggtcg 5341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5881 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 5941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 6001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 6061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 6121 actcacgtta agggattttg gtcatgcatt ctaggtgatt atttgccgac taccttggtg 6181 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga 6241 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc 6301 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact 6361 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcacc agcccagtcg 6421 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga 6481 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct 6541 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga 6601 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga 6661 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca 6721 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc 6781 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact 6841 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca 6901 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat aatgtttaac 6961 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat 7021 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa 7081 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc 7141 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa 7201 caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca 7261 accttgggta gcagcgaagt cgaggcattt ctgtcctggc tgg //