LOCUS OP_012 6582 bp DNA circular 29-Apr-2025 ACCESSION OP_012 VERSION OP_012.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(693..699) /label=SapI recognition site oriT 4765..4905 /label=basis of mobility region from pBR322 rep_origin 5091..5679 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 2030..2659 /label=pVS1 StaA stability protein CDS 3088..4161 /label=pVS1 RepA replication protein rep_origin 4227..4421 /label=pVS1 oriV origin of replication misc_feature 1791..2791 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 705..729 /label=A.tumefaciens plasmid right boundary CDS complement(5767..6582) /label=nptI / KanR misc_feature 484..683 /label=Odd-spacer rep_origin complement(5076..5690) /label=rep (pMB1) ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcacagag 481 gagTCTGAGC AGGACAACTC GCGTAGTGAG AGTTACATGT TCGTTGGGTT CTTCCGACAC 541 GGACCTGAGT TGGCCAACGT CCCACCTGAG GTCTGTGCCC CGGTGATGAG AAGTGTGCAT 601 CTCGTTCTTG CAGCTCGTCA GTACTTTCAG AATCATGGCG TGCATGGTAG AATGACCCTT 661 ATAACGGACT TCGACATGGC AATcgctagg ttgaagagca attctgacag gatatattgg 721 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga 781 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat 841 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg 901 cagccgtcat ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc 961 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc 1021 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat 1081 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc 1141 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg 1201 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc 1261 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg 1321 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg 1381 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg 1441 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc 1501 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag 1561 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag 1621 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag 1681 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc 1741 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg 1801 ccgggtacgt gttcgagccg cccgcgcacc tctcaaccgt gcggctgcat gaaatcctgg 1861 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg 1921 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg 1981 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat 2041 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg 2101 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc 2161 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc 2221 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc 2281 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc 2341 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca 2401 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa 2461 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct 2521 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt 2581 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat 2641 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg 2701 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca 2761 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg 2821 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg 2881 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg 2941 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg 3001 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac 3061 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa 3121 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag 3181 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga 3241 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc 3301 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat 3361 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc 3421 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc 3481 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc 3541 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt 3601 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta 3661 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag 3721 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta 3781 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc 3841 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc 3901 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa 3961 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag 4021 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag 4081 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc 4141 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg 4201 gaaaaaggtc gaaaaggact ctttcctgtg gatagcacgt acattgggaa cccaaagccg 4261 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca 4321 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta 4381 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca 4441 gccgaagtgc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct 4501 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat 4561 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc 4621 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccggt 4681 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 4741 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 4801 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 4861 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc atccgcttcc 4921 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4981 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 5041 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5101 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 5161 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 5221 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 5281 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5341 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5401 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5461 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5521 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5581 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 5641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 5701 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct 5761 aggtgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt 5821 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca 5881 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat 5941 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt 6001 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac 6061 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg 6121 tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg 6181 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc 6241 aggatattct tctaatacct ggaatgctgt tttccctggg atcgcagtgg tgagtaacca 6301 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag 6361 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt 6421 cagaaacaac tctggcgcat cgggcttccc atacaatcgg tagattgtcg cacctgattg 6481 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa 6541 tcgcggcctt gagcaagacg tttcccgttg aatatggctc at //