LOCUS       OP_017                  2641 bp    DNA     circular     29-Apr-2025
ACCESSION   OP_017
VERSION     OP_017.1  
FEATURES             Location/Qualifiers
     misc_feature    236..241
                     /label=BsaI recognition site
     misc_feature    complement(832..837)
                     /label=BsaI recognition site
     misc_feature    825..831
                     /label=SapI recognition site
     misc_feature    complement(242..248)
                     /label=SapI recognition site
     rep_origin      complement(1096..1684)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
     CDS             complement(1982..2641)
                     /label=cat / CmR
     terminator      complement(1867..1961)
                     /label=lambda t0 terminator
     promoter        complement(705..734)
                     /label=lac promoter
     misc_feature    complement(678..698)
                     /label=lac operator
     CDS             complement(339..660)
                     /label=LacZa
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGAAGAGCca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
      301 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
      361 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      421 atcaggcgct catccgccat tcaggctgcg caactgttgg gaagggcgat cggcgcaggc
      481 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      541 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg
      601 tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat
      661 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      721 gcatatagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      781 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgGCTCTT CgagaccCTG
      841 CAGTCCGGCA AAAAAGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG
      901 ATTACTTCGC GTTATGCAGG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
      961 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
     1021 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
     1081 CGCGTTGCTG GCGTTTTTCC ACAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1141 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1201 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1261 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1321 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1381 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1441 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1501 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1561 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1621 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1681 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     1741 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     1801 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCTCGAG
     1861 GCTTGGATTC TCACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG
     1921 ATGGAGTTCT GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA
     1981 ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA
     2041 CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT
     2101 CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG
     2161 CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT
     2221 TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG
     2281 AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG
     2341 TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT
     2401 CACCGTCTTT CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT
     2461 GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA
     2521 TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT
     2581 GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA
     2641 T
//