LOCUS OP_021 3711 bp DNA circular 14-Dec-2025 ACCESSION OP_021 VERSION OP_021.1 FEATURES Location/Qualifiers misc_feature 236..241 /label=BsaI recognition site misc_feature complement(1902..1907) /label=BsaI recognition site rep_origin complement(2166..2754) /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication CDS complement(3052..3711) /label=cat / CmR terminator complement(2937..3031) /label=lambda t0 terminator CDS 247..1896 /label=firefly luciferase ORIGIN 1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT 61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG 121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC 181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct 241 cAaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 301 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 361 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 421 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 481 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 541 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 601 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 661 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 721 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 781 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 841 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 901 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 961 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 1021 tttcgagtcg tcttaatgta tagatttgaa gaggagctgt ttctgaggag ccttcaggat 1081 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 1141 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 1201 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 1261 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 1321 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 1381 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 1441 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 1501 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 1561 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 1621 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 1681 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 1741 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 1801 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 1861 ataaaggcca agaagggcgg aaagatcgcc gtgtaagctt CgagaccCTG CAGTCCGGCA 1921 AAAAAGGGCA AGGTGTCACC ACCCTGCCCT TTTTCTTTAA AACCGAAAAG ATTACTTCGC 1981 GTTATGCAGG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 2041 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 2101 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 2161 GCGTTTTTCC ACAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 2221 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 2281 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 2341 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 2401 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 2461 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 2521 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 2581 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 2641 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 2701 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 2761 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 2821 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 2881 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCTCGAG GCTTGGATTC 2941 TCACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT 3001 GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC 3061 GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC 3121 ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT 3181 ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA 3241 ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA 3301 CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG 3361 TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG 3421 CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT 3481 CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC 3541 CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG 3601 AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG 3661 ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA T //