LOCUS OP_034 2814 bp DNA circular 29-Apr-2025 ACCESSION OP_034 VERSION OP_034.1 FEATURES Location/Qualifiers misc_feature 236..241 /label=BsaI recognition site misc_feature complement(1005..1010) /label=BsaI recognition site rep_origin complement(1269..1857) /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication CDS complement(2155..2814) /label=cat / CmR terminator complement(2040..2134) /label=lambda t0 terminator CDS 247..960 /label=eGFP no ATG no Stop misc_feature 961..984 /label=Gly-His tag ORIGIN 1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT 61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG 121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC 181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCGGTCT 241 CAttcggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 301 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 361 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 421 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 481 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 541 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 601 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 661 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 721 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 781 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 841 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 901 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 961 GGGGGGGGGC ACCACCACCA CCACCACGAT GAGCTTtaag cttCGAGACC CTGCAGTCCG 1021 GCAAAAAAGG GCAAGGTGTC ACCACCCTGC CCTTTTTCTT TAAAACCGAA AAGATTACTT 1081 CGCGTTATGC AGGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA 1141 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 1201 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 1261 CTGGCGTTTT TCCACAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT 1321 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 1381 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT 1441 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 1501 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 1561 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA 1621 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 1681 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG 1741 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 1801 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 1861 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 1921 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 1981 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGCTC GAGGCTTGGA 2041 TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT 2101 TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT CAAATTACGC 2161 CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA 2221 GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG 2281 CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT 2341 TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT 2401 AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT 2461 GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT 2521 TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC 2581 TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA 2641 GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG 2701 CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT 2761 ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCAT //