LOCUS       OP_050                  2867 bp    DNA     circular     29-Apr-2025
ACCESSION   OP_050
VERSION     OP_050.1  
FEATURES             Location/Qualifiers
     misc_feature    236..241
                     /label=BsaI recognition site
     misc_feature    complement(1058..1063)
                     /label=BsaI recognition site
     rep_origin      complement(1322..1910)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
     CDS             complement(2208..2867)
                     /label=cat / CmR
     terminator      complement(2093..2187)
                     /label=lambda t0 terminator
     misc_feature    974..980
                     /label=TATA box
     promoter        958..1003
                     /label=min 35S pro
     misc_feature    263..587
                     /label=35S
     misc_feature    588..912
                     /label=35S
     promoter        263..1013
                     /label=p5-35Sx2
     promoter        247..1052
                     /label=p5-35Sx2
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cTGGAGgcta gagcagcttg ccaacatggt ggagcacgac actctcgtct actccaagaa
      301 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      361 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      421 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      481 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      541 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc
      601 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
      661 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      721 tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa tgccatcatt
      781 gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc aaagatggac
      841 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      901 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      961 aagaCccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tgaaatcacc
     1021 agtctctctc tacaaatcta tctctctcga gcAATGTgag accCTGCAGT CCGGCAAAAA
     1081 AGGGCAAGGT GTCACCACCC TGCCCTTTTT CTTTAAAACC GAAAAGATTA CTTCGCGTTA
     1141 TGCAGGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
     1201 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA
     1261 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
     1321 TTTTCCACAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
     1381 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
     1441 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
     1501 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
     1561 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
     1621 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
     1681 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
     1741 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
     1801 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
     1861 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
     1921 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
     1981 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
     2041 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG CTCGAGGCTT GGATTCTCAC
     2101 CAATAAAAAA CGCCCGGCGG CAACCGAGCG TTCTGAACAA ATCCAGATGG AGTTCTGAGG
     2161 TCATTACTGG ATCTATCAAC AGGAGTCCAA GCGAGCTCGA TATCAAATTA CGCCCCGCCC
     2221 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA
     2281 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA
     2341 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA
     2401 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT
     2461 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA
     2521 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA
     2581 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT
     2641 GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA
     2701 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG
     2761 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC
     2821 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCAT
//