LOCUS       OP_051                  3795 bp    DNA     circular     29-Apr-2025
ACCESSION   OP_051
VERSION     OP_051.1  
FEATURES             Location/Qualifiers
     misc_feature    236..241
                     /label=BsaI recognition site
     misc_feature    complement(1986..1991)
                     /label=BsaI recognition site
     rep_origin      complement(2250..2838)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
     CDS             complement(3136..3795)
                     /label=cat / CmR
     terminator      complement(3021..3115)
                     /label=lambda t0 terminator
     misc_feature    1202..1208
                     /label=TATA box
     promoter        247..1248
                     /label=PROM_MpEF1a
     misc_feature    1608..1967
                     /label=Intron in 5UTR_MpEF1a
     misc_feature    1968..1980
                     /label=exon 5UTR_MpEF1a
     misc_feature    1249..1607
                     /label=exon 5UTR_MpEF1a
     promoter        247..1980
                     /label=PROM5_MpEF1a
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cAggagcaaa tgagtcacac acattgttga gagacatatc aaaataactc aattgtaagg
      301 taagttgata gatttcccag ttaaatgcat tattttaaca atatttgtat ctcaaatttt
      361 caaaatcata tcaaatttgc agatcttgat gatgattcac aactctgtct tcgatttttg
      421 attgttaacg ggtatcgata tttgggtaac ttaaaaagaa tttgctaaag atcttaaaaa
      481 acagattaaa aaaacctttc aaaatagcta atcctttaaa tccgtttcac aatataatgc
      541 tcattgatga taatcgaaaa gattccattt taaagtgcat ataaattgct taattatctc
      601 tttctgtctc gtaatatttt gttctctcct attggatcgg acccgcatct tctcgatcag
      661 ttcactatac taatatttgg tcaacttcaa gagagattag aacttcgttc tccaaagtgt
      721 aaaggccgac aagcagaaaa ggaaacttca atcagaagcc ttctaggttt tcccgcgtcc
      781 caaaaagcga agggaggacg gggaggacgg tgaggccaga agaagaaagc aacagtaaaa
      841 tctcatgtga ttgcgtgcat gtcatggatc tatgggatgc tagtccaccg tttggaagta
      901 tgtgcagaag gagaaaggcg cgggaggcca aaagcgactt cggccacctc tctactttct
      961 agaaagtttg ctcgcagctt gtaataagcg cggaaaaggt actcgaaaaa ccggagcaat
     1021 cggtgtggta tcagcagtcg ggttcgctaa acctacgtct tcttattttg gaatgttaaa
     1081 ttgaaagggc gtgccggccc taagcacgga cgatgtgaca gacggaggag tgtgagcaat
     1141 tcggatgacg atgctggccg gcagtcacga caaagagtcc gaaggggcat ggacagggca
     1201 atatataaat ggaaaagagg ctttgaatat tcctaaccct tcgtcgtcgg cctcccaact
     1261 tgccctcgca tgctgaattg gccgcctatc tgcgaatggc cggccgccga ccctaagtcg
     1321 caataacttg aacggagccg atgcaaagtc cttatcgaac gctcgttatt ataaacgggg
     1381 cagacctagg tatttaatta tatcaaaacc ctccgcaatt tcgctaatca ttccctaatt
     1441 tcttctactt tggcgtctag tgctgtggtc gcgcttgctc tcaaagctcg ctgggaggca
     1501 gtggagcgtc tggcttaaaa tcgatcgccg caaccctaga gttcctttca cttctttctc
     1561 tcttgcgtgg agagcatcga catcgaggag ctgcatcttg tcttctggta tgcgccttgt
     1621 gccctcgtct ctgtacgtcg ttacgattgc tcgatattgt gattgtctag acctgaaacc
     1681 cgactggagc ttttacgtgg tgtcgattgg ggcctcgagc gagtgatttt ttaggttcgt
     1741 tttagccaag tacatcccac tttggtcagt cctgttatct cggtaaggtg ttcagttcgg
     1801 tcactggtgc ggaggtgcct cgtaagtttc ctgcatgata gatttgtcta tccttgactt
     1861 gaagaggagt cgaaggtcag cgaggttggc tgtgtgctat ggacgtcggt gctccttggt
     1921 tccgtgcttc tatgggtgtt gcctcaacag tattgatgtg cctgcagaaa ggttgtcacc
     1981 aatgCgagac cCTGCAGTCC GGCAAAAAAG GGCAAGGTGT CACCACCCTG CCCTTTTTCT
     2041 TTAAAACCGA AAAGATTACT TCGCGTTATG CAGGCTTCCT CGCTCACTGA CTCGCTGCGC
     2101 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     2161 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     2221 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCACAGGC TCCGCCCCCC TGACGAGCAT
     2281 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     2341 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     2401 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     2461 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     2521 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     2581 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     2641 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
     2701 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     2761 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     2821 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2881 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2941 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     3001 TCTGACAGCT CGAGGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT
     3061 CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC
     3121 GAGCTCGATA TCAAATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT
     3181 AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG
     3241 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA
     3301 GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA
     3361 GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA
     3421 CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA
     3481 GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC
     3541 CCATATCACC AGCTCACCGT CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG
     3601 GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT
     3661 TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG
     3721 AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT
     3781 GATTTTTTTC TCCAT
//