LOCUS       OP_052                  2265 bp    DNA     circular     29-Apr-2025
ACCESSION   OP_052
VERSION     OP_052.1  
FEATURES             Location/Qualifiers
     misc_feature    1177..1182
                     /label=BsaI recognition site
     misc_feature    complement(1397..1402)
                     /label=BsaI recognition site
     rep_origin      complement(1661..2249)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
     CDS             complement(282..941)
                     /label=cat / CmR
     terminator      complement(167..261)
                     /label=lambda t0 terminator
     terminator      1188..1391
                     /label=3TERM_35S (MoClo)
ORIGIN
        1 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
       61 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
      121 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCTCGAG GCTTGGATTC TCACCAATAA
      181 AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT GAGGTCATTA
      241 CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC
      301 TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG
      361 GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG
      421 CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG
      481 GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA CCCTTTAGGG
      541 AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC
      601 CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA
      661 ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA
      721 CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC
      781 TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG
      841 TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG
      901 GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT
      961 GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG
     1021 TTGGAACCTC TTACGTGCCC GATCAACTCG AGTGCCACCT GACGTCTAAG AAACCATTAT
     1081 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CAGAATTTCA GATAAAAAAA
     1141 ATCCTTAGCT TTCGCTAAGG ATGATTTCTG GAATTCggtc tcGGCTTctc tagctagagt
     1201 cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
     1261 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     1321 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaCcaaa atccagtact
     1381 aaaatccaga tCGCTTgaga ccCTGCAGTC CGGCAAAAAA GGGCAAGGTG TCACCACCCT
     1441 GCCCTTTTTC TTTAAAACCG AAAAGATTAC TTCGCGTTAT GCAGGCTTCC TCGCTCACTG
     1501 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
     1561 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
     1621 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCACAGG CTCCGCCCCC
     1681 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
     1741 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
     1801 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
     1861 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
     1921 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
     1981 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
     2041 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
     2101 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
     2161 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
     2221 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCT
//