LOCUS       OP_053                  2324 bp    DNA     circular     29-Apr-2025
ACCESSION   OP_053
VERSION     OP_053.1  
FEATURES             Location/Qualifiers
     misc_feature    236..241
                     /label=BsaI recognition site
     misc_feature    complement(515..520)
                     /label=BsaI recognition site
     rep_origin      complement(779..1367)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
     CDS             complement(1665..2324)
                     /label=cat / CmR
     terminator      complement(1550..1644)
                     /label=lambda t0 terminator
     misc_feature    255..508
                     /label=nosT (MoClo)
     terminator      247..509
                     /label=3TERM_Nos
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGGCTTgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
      301 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
      361 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
      421 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
      481 gcgcggtgtc atctatgtta ctagatcgaC GCTTgagacc CTGCAGTCCG GCAAAAAAGG
      541 GCAAGGTGTC ACCACCCTGC CCTTTTTCTT TAAAACCGAA AAGATTACTT CGCGTTATGC
      601 AGGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG
      661 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA
      721 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT
      781 TCCACAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
      841 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
      901 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
      961 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
     1021 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
     1081 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
     1141 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
     1201 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
     1261 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
     1321 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA
     1381 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA
     1441 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT
     1501 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGCTC GAGGCTTGGA TTCTCACCAA
     1561 TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT TCTGAGGTCA
     1621 TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC
     1681 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA
     1741 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT
     1801 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA
     1861 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA
     1921 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC
     1981 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG
     2041 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC
     2101 ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA
     2161 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC
     2221 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT
     2281 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCAT
//