LOCUS OP_058 4069 bp DNA circular 29-Apr-2025 ACCESSION OP_058 VERSION OP_058.1 FEATURES Location/Qualifiers misc_feature 236..241 /label=BsaI recognition site misc_feature complement(2260..2265) /label=BsaI recognition site rep_origin complement(2524..3112) /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication CDS complement(3410..4069) /label=cat / CmR terminator complement(3295..3389) /label=lambda t0 terminator promoter 247..2258 /label=p5-MpU6 Sugano ORIGIN 1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT 61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG 121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC 181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct 241 cGGGAGTATT CATTCAAAAG AGATTTTTAA AGATCAATTT TTCTTTCTCA TAAGTCTAAT 301 ACGTCATGAT AATAACTTTA AAAAAAATTG AACTCGTTAA AAAATTTGAT CAAATTTTAA 361 TTTATTATTA GATCATATAT TACATATCAG TTTCATAATG TTATACTAAG ATCTTTCATA 421 TATATTTTAA CCCACGAGAC AAAATATGTT TATATCTTTA TTTATTTAAA TATTTTTTAG 481 CTCAAAACAT CGCAAACTCA GCTATCATAA TGCTTTTTAA TGAAAAAAAC AAATTTTCCT 541 ATCATACTCT CATTGTTTAT AATTTTTTAG AAAAAAAACT TGAAGATAAA ATAACAACAA 601 TAATAGTTTC AAAAGCACAT TTTTGTTGTA AATTTCAAGA AAAATACAAT CATATTACTG 661 ATTGACTACA AAAATTTGTA AATTAATCCA GGCTACATGT TTAAGATGTG TCAAAACTGC 721 ATCATCTACA TCTCATTTTA ATTTTTCTAA ACTACATACA TAGTATCAAT AACAATTGAA 781 CAATGCTAGT TAATGTGTTA TACTCATTAG CTTGTAATAT ATCTTCAACT TGATGAATCG 841 GATTGATCAT AAAATTTCAC AAACTTATGT GCTGATTAAT AAAAAATATA ATCAGATTTT 901 TCATGAATAA TTAAGATAAA TAACACATGC AAGTCTCAAT TAGAATTTAA CAATCTGACT 961 ATAAAAAGTC GGTTTATCTT GATTTTCACA AAAATTTAAC ATAGTTTCAA GGTGAATGGG 1021 CTTTTATTTT AATCCAATCA ACTATTTGAC ACATACATCT TGCAAATTTA TTTATTTTTT 1081 ATGGAAAAAA ACATGATTTA TGTTTTTCTT TGAGAAAGTA TATTGTGGAT TGTCAAAAAA 1141 AACCTATGGA AGATTTATAT GTAAGAGTTA AAAATTTATT TTCAAATATG TCAACAATCC 1201 ACAATATCGA AGAAAATTTT CAAAAAATCA TCACATATGA TGTAAATATA ATATGAATAT 1261 GTGCAATCTT ATTTCATTTT ACTCTGAAAA CTTAATTTCT TTTTATAGAG CATTCTGTAC 1321 TTACTGGTGT CAAATATACA GATAAAATGC AGTCCATAAC GTATCACTCT ATAAGCAAAA 1381 TCATAAACTT CGTGATCTCT CACAGTTCAA ACGAGAACTA TAGAAACCCT TCAACTAGCA 1441 AAAAACTCAC AACTTTCTAT TCCAATATCT TTAATTGTCT GACGATTTAC AAATAGAAAC 1501 GAAAACCAGA CACACACACA CACGCAAACT ACAAAGTAAA AACACAACCA CCTGACATTA 1561 GACAGTGAGA TTATTAAACA TCCTAAACTA AAAAACAAAC AAATTAATTC TCTATGTACA 1621 AGTCCCCATC GTGATCAATT TTTGCATCCC AATATAGTGG CGGTTAGTAT AAGAGGACCT 1681 TCGTCTATTC ACTCTCCATG TTTGTCAAAC TATAGTACAG TACATATATG TATGGGAACA 1741 AATTCCGAAC ACTCTAGTAC ACTCCTCAAC GCAAGTTCTA TCTCATATCT CTGCAAGAGC 1801 TTATTCCAAG AAACTTTCTT GTGCTTCTAT GCATGCCCAG GTACCTTAAG TCGCGCGCAT 1861 AGTCACTTCA CAGCCTTTTC AGTTCTCCCT TTGATGGCTC AGAGTGACGC AACTGCACAA 1921 AAATAAGAAA AAAGTCGAAG CGTCACGAGA GATGTATCCG GCTAGTTTCC TTGGAGTTCG 1981 GATTGCTCTC TTTCTCTGTC TCCCCGAACC ATATGGAATT TATGAACTGC TGTTCACTAG 2041 TCAGTCACTT GTTTCAGAAC TTTTGAGTGG TCGATTTATC CACCTTCTTG TTTCTTCCTG 2101 GGTCCAGAAT CTGACTTCTT GAGACAACAA ACAGTGCACA GTGGATCGAA GAATGACCAC 2161 AGTTGAGACA CGTGGCGCTG GCCTCATTTA TTGCACCATA CGTTTAGTGA AGAACTGAAT 2221 AATGGTGGAT ATAAAGCAGA GTTGCACCCA GCCTCTCGTg agaccCTGCA GTCCGGCAAA 2281 AAAGGGCAAG GTGTCACCAC CCTGCCCTTT TTCTTTAAAA CCGAAAAGAT TACTTCGCGT 2341 TATGCAGGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 2401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 2461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 2521 GTTTTTCCAC AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 2581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 2641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 2701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 2761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 2821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 2881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 2941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 3001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 3061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 3121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 3181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 3241 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCTCGAGGC TTGGATTCTC 3301 ACCAATAAAA AACGCCCGGC GGCAACCGAG CGTTCTGAAC AAATCCAGAT GGAGTTCTGA 3361 GGTCATTACT GGATCTATCA ACAGGAGTCC AAGCGAGCTC GATATCAAAT TACGCCCCGC 3421 CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT 3481 CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT 3541 AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT 3601 CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC 3661 CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA 3721 GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT 3781 CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA 3841 TTGCCATACG AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 3901 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA 3961 CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT 4021 GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCAT //