LOCUS OP_059 6198 bp DNA circular 29-Apr-2025 ACCESSION OP_059 VERSION OP_059.1 FEATURES Location/Qualifiers misc_feature 236..241 /label=BsaI recognition site misc_feature complement(4389..4394) /label=BsaI recognition site rep_origin complement(4653..5241) /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication CDS complement(5539..6198) /label=cat / CmR terminator complement(5424..5518) /label=lambda t0 terminator misc_feature 4360..4380 /label=NLS_SV40 CDS 244..4383 /label=AtcoCas9 (Puchta)STOP CDS 244..4380 /label=AtcoCas9 (Puctha) NoStop ORIGIN 1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT 61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG 121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC 181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct 241 cGAatggata agaagtactc tatcggactc gatatcggaa ctaactctgt gggatgggct 301 gtgatcaccg atgagtacaa ggtgccatct aagaagttca aggttctcgg aaacaccgat 361 aggcactcta tcaagaaaaa ccttatcggt gctctcctct tcgattctgg tgaaactgct 421 gaggctacca gactcaagag aaccgctaga agaaggtaca ccagaagaaa gaacaggatc 481 tgctacctcc aagagatctt ctctaacgag atggctaaag tggatgattc attcttccac 541 aggctcgaag agtcattcct cgtggaagaa gataagaagc acgagaggca ccctatcttc 601 ggaaacatcg ttgatgaggt ggcataccac gagaagtacc ctactatcta ccacctcaga 661 aagaagctcg ttgattctac tgataaggct gatctcaggc tcatctacct cgctctcgct 721 cacatgatca agttcagagg acacttcctc atcgagggtg atctcaaccc tgataactct 781 gatgtggata agttgttcat ccagctcgtg cagacctaca accagctttt cgaagagaac 841 cctatcaacg cttcaggtgt ggatgctaag gctatcctct ctgctaggct ctctaagtca 901 agaaggcttg agaacctcat tgctcagctc cctggtgaga agaagaacgg acttttcgga 961 aacttgatcg ctctctctct cggactcacc cctaacttca agtctaactt cgatctcgct 1021 gaggatgcaa agctccagct ctcaaaggat acctacgatg atgatctcga taacctcctc 1081 gctcagatcg gagatcagta cgctgatttg ttcctcgctg ctaagaacct ctctgatgct 1141 atcctcctca gtgatatcct cagagtgaac accgagatca ccaaggctcc actctcagct 1201 tctatgatca agagatacga tgagcaccac caggatctca cacttctcaa ggctcttgtt 1261 agacagcagc tcccagagaa gtacaaagag attttcttcg atcagtctaa gaacggatac 1321 gctggttaca tcgatggtgg tgcatctcaa gaagagttct acaagttcat caagcctatc 1381 ctcgagaaga tggatggaac cgaggaactc ctcgtgaagc tcaatagaga ggatcttctc 1441 agaaagcaga ggaccttcga taacggatct atccctcatc agatccacct cggagagttg 1501 cacgctatcc ttagaaggca agaggatttc tacccattcc tcaaggataa cagggaaaag 1561 attgagaaga ttctcacctt cagaatccct tactacgtgg gacctctcgc tagaggaaac 1621 tcaagattcg cttggatgac cagaaagtct gaggaaacca tcaccccttg gaacttcgaa 1681 gaggtggtgg ataagggtgc tagtgctcag tctttcatcg agaggatgac caacttcgat 1741 aagaaccttc caaacgagaa ggtgctccct aagcactctt tgctctacga gtacttcacc 1801 gtgtacaacg agttgaccaa ggttaagtac gtgaccgagg gaatgaggaa gcctgctttt 1861 ttgtcaggtg agcaaaagaa ggctatcgtt gatctcttgt tcaagaccaa cagaaaggtg 1921 accgtgaagc agctcaaaga ggattacttc aagaaaatcg agtgcttcga ttcagttgag 1981 atttctggtg ttgaggatag gttcaacgca tctctcggaa cctaccacga tctcctcaag 2041 atcattaagg ataaggattt cttggataac gaggaaaacg aggatatctt ggaggatatc 2101 gttcttaccc tcaccctctt tgaagataga gagatgattg aagaaaggct caagacctac 2161 gctcatctct tcgatgataa ggtgatgaag cagttgaaga gaagaagata cactggttgg 2221 ggaaggctct caagaaagct cattaacgga atcagggata agcagtctgg aaagacaatc 2281 cttgatttcc tcaagtctga tggattcgct aacagaaact tcatgcagct catccacgat 2341 gattctctca cctttaaaga ggatatccag aaggctcagg tttcaggaca gggtgatagt 2401 ctccatgagc atatcgctaa cctcgctgga tctcctgcaa tcaagaaggg aatcctccag 2461 actgtgaagg ttgtggatga gttggtgaag gtgatgggaa ggcataagcc tgagaacatc 2521 gtgatcgaaa tggctagaga gaaccagacc actcagaagg gacagaagaa ctctagggaa 2581 aggatgaaga ggatcgagga aggtatcaaa gagcttggat ctcagatcct caaagagcac 2641 cctgttgaga acactcagct ccagaatgag aagctctacc tctactacct ccagaacgga 2701 agggatatgt atgtggatca agagttggat atcaacaggc tctctgatta cgatgttgat 2761 catatcgtgc cacagtcatt cttgaaggat gattctatcg ataacaaggt gctcaccagg 2821 tctgataaga acaggggtaa gagtgataac gtgccaagtg aagaggttgt gaagaaaatg 2881 aagaactatt ggaggcagct cctcaacgct aagctcatca ctcagagaaa gttcgataac 2941 ttgactaagg ctgagagggg aggactctct gaattggata aggcaggatt catcaagagg 3001 cagcttgtgg aaaccaggca gatcactaag cacgttgcac agatcctcga ttctaggatg 3061 aacaccaagt acgatgagaa cgataagttg atcagggaag tgaaggttat caccctcaag 3121 tcaaagctcg tgtctgattt cagaaaggat ttccaattct acaaggtgag ggaaatcaac 3181 aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc tctcatcaag 3241 aagtatccta agctcgagtc agagttcgtg tacggtgatt acaaggtgta cgatgtgagg 3301 aagatgatcg ctaagtctga gcaagagatc ggaaaggcta ccgctaagta tttcttctac 3361 tctaacatca tgaatttctt caagaccgag attaccctcg ctaacggtga gatcagaaag 3421 aggccactca tcgagacaaa cggtgaaaca ggtgagatcg tgtgggataa gggaagggat 3481 ttcgctaccg ttagaaaggt gctctctatg ccacaggtga acatcgttaa gaaaaccgag 3541 gtgcagaccg gtggattctc taaagagtct atcctcccta agaggaactc tgataagctc 3601 attgctagga agaaggattg ggaccctaag aaatacggtg gtttcgattc tcctaccgtg 3661 gcttactctg ttctcgttgt ggctaaggtt gagaagggaa agagtaagaa gctcaagtct 3721 gttaaggaac ttctcggaat cactatcatg gaaaggtcat ctttcgagaa gaacccaatc 3781 gatttcctcg aggctaaggg atacaaagag gttaagaagg atctcatcat caagctccca 3841 aagtactcac tcttcgaact cgagaacggt agaaagagga tgctcgcttc tgctggtgag 3901 cttcaaaagg gaaacgagct tgctctccca tctaagtacg ttaactttct ttacctcgct 3961 tctcactacg agaagttgaa gggatctcca gaagataacg agcagaagca acttttcgtt 4021 gagcagcaca agcactactt ggatgagatc atcgagcaga tctctgagtt ctctaaaagg 4081 gtgatcctcg ctgatgcaaa cctcgataag gtgttgtctg cttacaacaa gcacagagat 4141 aagcctatca gggaacaggc agagaacatc atccatctct tcacccttac caacctcggt 4201 gctcctgctg ctttcaagta cttcgataca accatcgata ggaagagata cacctctacc 4261 aaagaagtgc tcgatgctac cctcatccat cagtctatca ctggactcta cgagactagg 4321 atcgatctct cacagctcgg tggtgattca agggctgatc ctaagaagaa gaggaaggtt 4381 tgaGCTTTga gaccCTGCAG TCCGGCAAAA AAGGGCAAGG TGTCACCACC CTGCCCTTTT 4441 TCTTTAAAAC CGAAAAGATT ACTTCGCGTT ATGCAGGCTT CCTCGCTCAC TGACTCGCTG 4501 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 4561 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 4621 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCACA GGCTCCGCCC CCCTGACGAG 4681 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 4741 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 4801 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 4861 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 4921 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 4981 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 5041 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 5101 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 5161 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 5221 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 5281 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 5341 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 5401 TGGTCTGACA GCTCGAGGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC 5461 GTTCTGAACA AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA 5521 AGCGAGCTCG ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC 5581 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG 5641 CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA 5701 GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC 5761 TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA 5821 ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT 5881 CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT 5941 ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT 6001 CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT 6061 CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA 6121 CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC 6181 AGTGATTTTT TTCTCCAT //