LOCUS OP_060 6197 bp DNA circular 17-Jun-2025 ACCESSION OP_060 VERSION OP_060.1 FEATURES Location/Qualifiers misc_feature 259..264 /label=BsaI recognition site misc_feature complement(4411..4416) /label=BsaI recognition site rep_origin 5609..6197 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication CDS 4652..5311 /label=cat / CmR terminator 5332..5426 /label=lambda t0 terminator misc_feature 4383..4403 /label=NLS_SV40 CDS 267..4403 /label=AtcoCas9 (Puctha) NoStop ORIGIN 1 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG 61 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 121 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCCTGC 181 ATAACGCGAA GTAATCTTTT CGGTTTTAAA GAAAAAGGGC AGGGTGGTGA CACCTTGCCC 241 TTTTTTGCCG GACTGCAGgg tctcGAatgg ataagaagta ctctatcgga ctcgatatcg 301 gaactaactc tgtgggatgg gctgtgatca ccgatgagta caaggtgcca tctaagaagt 361 tcaaggttct cggaaacacc gataggcact ctatcaagaa aaaccttatc ggtgctctcc 421 tcttcgattc tggtgaaact gctgaggcta ccagactcaa gagaaccgct agaagaaggt 481 acaccagaag aaagaacagg atctgctacc tccaagagat cttctctaac gagatggcta 541 aagtggatga ttcattcttc cacaggctcg aagagtcatt cctcgtggaa gaagataaga 601 agcacgagag gcaccctatc ttcggaaaca tcgttgatga ggtggcatac cacgagaagt 661 accctactat ctaccacctc agaaagaagc tcgttgattc tactgataag gctgatctca 721 ggctcatcta cctcgctctc gctcacatga tcaagttcag aggacacttc ctcatcgagg 781 gtgatctcaa ccctgataac tctgatgtgg ataagttgtt catccagctc gtgcagacct 841 acaaccagct tttcgaagag aaccctatca acgcttcagg tgtggatgct aaggctatcc 901 tctctgctag gctctctaag tcaagaaggc ttgagaacct cattgctcag ctccctggtg 961 agaagaagaa cggacttttc ggaaacttga tcgctctctc tctcggactc acccctaact 1021 tcaagtctaa cttcgatctc gctgaggatg caaagctcca gctctcaaag gatacctacg 1081 atgatgatct cgataacctc ctcgctcaga tcggagatca gtacgctgat ttgttcctcg 1141 ctgctaagaa cctctctgat gctatcctcc tcagtgatat cctcagagtg aacaccgaga 1201 tcaccaaggc tccactctca gcttctatga tcaagagata cgatgagcac caccaggatc 1261 tcacacttct caaggctctt gttagacagc agctcccaga gaagtacaaa gagattttct 1321 tcgatcagtc taagaacgga tacgctggtt acatcgatgg tggtgcatct caagaagagt 1381 tctacaagtt catcaagcct atcctcgaga agatggatgg aaccgaggaa ctcctcgtga 1441 agctcaatag agaggatctt ctcagaaagc agaggacctt cgataacgga tctatccctc 1501 atcagatcca cctcggagag ttgcacgcta tccttagaag gcaagaggat ttctacccat 1561 tcctcaagga taacagggaa aagattgaga agattctcac cttcagaatc ccttactacg 1621 tgggacctct cgctagagga aactcaagat tcgcttggat gaccagaaag tctgaggaaa 1681 ccatcacccc ttggaacttc gaagaggtgg tggataaggg tgctagtgct cagtctttca 1741 tcgagaggat gaccaacttc gataagaacc ttccaaacga gaaggtgctc cctaagcact 1801 ctttgctcta cgagtacttc accgtgtaca acgagttgac caaggttaag tacgtgaccg 1861 agggaatgag gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc gttgatctct 1921 tgttcaagac caacagaaag gtgaccgtga agcagctcaa agaggattac ttcaagaaaa 1981 tcgagtgctt cgattcagtt gagatttctg gtgttgagga taggttcaac gcatctctcg 2041 gaacctacca cgatctcctc aagatcatta aggataagga tttcttggat aacgaggaaa 2101 acgaggatat cttggaggat atcgttctta ccctcaccct ctttgaagat agagagatga 2161 ttgaagaaag gctcaagacc tacgctcatc tcttcgatga taaggtgatg aagcagttga 2221 agagaagaag atacactggt tggggaaggc tctcaagaaa gctcattaac ggaatcaggg 2281 ataagcagtc tggaaagaca atccttgatt tcctcaagtc tgatggattc gctaacagaa 2341 acttcatgca gctcatccac gatgattctc tcacctttaa agaggatatc cagaaggctc 2401 aggtttcagg acagggtgat agtctccatg agcatatcgc taacctcgct ggatctcctg 2461 caatcaagaa gggaatcctc cagactgtga aggttgtgga tgagttggtg aaggtgatgg 2521 gaaggcataa gcctgagaac atcgtgatcg aaatggctag agagaaccag accactcaga 2581 agggacagaa gaactctagg gaaaggatga agaggatcga ggaaggtatc aaagagcttg 2641 gatctcagat cctcaaagag caccctgttg agaacactca gctccagaat gagaagctct 2701 acctctacta cctccagaac ggaagggata tgtatgtgga tcaagagttg gatatcaaca 2761 ggctctctga ttacgatgtt gatcatatcg tgccacagtc attcttgaag gatgattcta 2821 tcgataacaa ggtgctcacc aggtctgata agaacagggg taagagtgat aacgtgccaa 2881 gtgaagaggt tgtgaagaaa atgaagaact attggaggca gctcctcaac gctaagctca 2941 tcactcagag aaagttcgat aacttgacta aggctgagag gggaggactc tctgaattgg 3001 ataaggcagg attcatcaag aggcagcttg tggaaaccag gcagatcact aagcacgttg 3061 cacagatcct cgattctagg atgaacacca agtacgatga gaacgataag ttgatcaggg 3121 aagtgaaggt tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag gatttccaat 3181 tctacaaggt gagggaaatc aacaactacc accacgctca cgatgcttac cttaacgctg 3241 ttgttggaac cgctctcatc aagaagtatc ctaagctcga gtcagagttc gtgtacggtg 3301 attacaaggt gtacgatgtg aggaagatga tcgctaagtc tgagcaagag atcggaaagg 3361 ctaccgctaa gtatttcttc tactctaaca tcatgaattt cttcaagacc gagattaccc 3421 tcgctaacgg tgagatcaga aagaggccac tcatcgagac aaacggtgaa acaggtgaga 3481 tcgtgtggga taagggaagg gatttcgcta ccgttagaaa ggtgctctct atgccacagg 3541 tgaacatcgt taagaaaacc gaggtgcaga ccggtggatt ctctaaagag tctatcctcc 3601 ctaagaggaa ctctgataag ctcattgcta ggaagaagga ttgggaccct aagaaatacg 3661 gtggtttcga ttctcctacc gtggcttact ctgttctcgt tgtggctaag gttgagaagg 3721 gaaagagtaa gaagctcaag tctgttaagg aacttctcgg aatcactatc atggaaaggt 3781 catctttcga gaagaaccca atcgatttcc tcgaggctaa gggatacaaa gaggttaaga 3841 aggatctcat catcaagctc ccaaagtact cactcttcga actcgagaac ggtagaaaga 3901 ggatgctcgc ttctgctggt gagcttcaaa agggaaacga gcttgctctc ccatctaagt 3961 acgttaactt tctttacctc gcttctcact acgagaagtt gaagggatct ccagaagata 4021 acgagcagaa gcaacttttc gttgagcagc acaagcacta cttggatgag atcatcgagc 4081 agatctctga gttctctaaa agggtgatcc tcgctgatgc aaacctcgat aaggtgttgt 4141 ctgcttacaa caagcacaga gataagccta tcagggaaca ggcagagaac atcatccatc 4201 tcttcaccct taccaacctc ggtgctcctg ctgctttcaa gtacttcgat acaaccatcg 4261 ataggaagag atacacctct accaaagaag tgctcgatgc taccctcatc catcagtcta 4321 tcactggact ctacgagact aggatcgatc tctcacagct cggtggtgat tcaagggctg 4381 atcctaagaa gaagaggaag gttgcTTCGT gagaccGAAT TCCAGAAATC ATCCTTAGCG 4441 AAAGCTAAGG ATTTTTTTTA TCTGAAATTC TGCCTCGTGA TACGCCTATT TTTATAGGTT 4501 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTCGAGTTGA TCGGGCACGT 4561 AAGAGGTTCC AACTTTCACC ATAATGAAAT AAGATCACTA CCGGGCGTAT TTTTTGAGTT 4621 ATCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC 4681 CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA 4741 ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA 4801 AAATAAGCAC AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA 4861 TCCGGAATTT CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC 4921 TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA 4981 CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA 5041 CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG 5101 GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT 5161 TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA 5221 GGTTCATCAT GCCGTTTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA 5281 GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTGATATC GAGCTCGCTT GGACTCCTGT 5341 TGATAGATCC AGTAATGACC TCAGAACTCC ATCTGGATTT GTTCAGAACG CTCGGTTGCC 5401 GCCGGGCGTT TTTTATTGGT GAGAATCCAA GCCTCGAGCT GTCAGACCAA GTTTACTCAT 5461 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC 5521 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG 5581 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT 5641 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC 5701 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC 5761 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG 5821 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT 5881 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT 5941 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC 6001 TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 6061 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA 6121 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG 6181 GGCGGAGCCT GTGGAAA //