LOCUS OP_061 9123 bp DNA circular 29-Apr-2025 ACCESSION OP_061 VERSION OP_061.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(3234..3240) /label=SapI recognition site oriT 7306..7446 /label=basis of mobility region from pBR322 rep_origin 7632..8220 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 4571..5200 /label=pVS1 StaA stability protein CDS 5629..6702 /label=pVS1 RepA replication protein rep_origin 6768..6962 /label=pVS1 oriV origin of replication misc_feature 4332..5332 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 3246..3270 /label=A.tumefaciens plasmid right boundary CDS complement(8308..9123) /label=nptI / KanR terminator 3021..3224 /label=3TERM_35S (MoClo) misc_feature 1439..1445 /label=TATA box promoter 484..1485 /label=PROM_MpEF1a misc_feature 1845..2204 /label=Intron in 5UTR_MpEF1a misc_feature 2205..2217 /label=exon 5UTR_MpEF1a misc_feature 1486..1844 /label=exon 5UTR_MpEF1a promoter 484..2217 /label=PROM5_MpEF1a rep_origin complement(7617..8231) /label=rep (pMB1) CDS 2219..3016 /label=nptII / KanR ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcaatgag 481 gagcaaatga gtcacacaca ttgttgagag acatatcaaa ataactcaat tgtaaggtaa 541 gttgatagat ttcccagtta aatgcattat tttaacaata tttgtatctc aaattttcaa 601 aatcatatca aatttgcaga tcttgatgat gattcacaac tctgtcttcg atttttgatt 661 gttaacgggt atcgatattt gggtaactta aaaagaattt gctaaagatc ttaaaaaaca 721 gattaaaaaa acctttcaaa atagctaatc ctttaaatcc gtttcacaat ataatgctca 781 ttgatgataa tcgaaaagat tccattttaa agtgcatata aattgcttaa ttatctcttt 841 ctgtctcgta atattttgtt ctctcctatt ggatcggacc cgcatcttct cgatcagttc 901 actatactaa tatttggtca acttcaagag agattagaac ttcgttctcc aaagtgtaaa 961 ggccgacaag cagaaaagga aacttcaatc agaagccttc taggttttcc cgcgtcccaa 1021 aaagcgaagg gaggacgggg aggacggtga ggccagaaga agaaagcaac agtaaaatct 1081 catgtgattg cgtgcatgtc atggatctat gggatgctag tccaccgttt ggaagtatgt 1141 gcagaaggag aaaggcgcgg gaggccaaaa gcgacttcgg ccacctctct actttctaga 1201 aagtttgctc gcagcttgta ataagcgcgg aaaaggtact cgaaaaaccg gagcaatcgg 1261 tgtggtatca gcagtcgggt tcgctaaacc tacgtcttct tattttggaa tgttaaattg 1321 aaagggcgtg ccggccctaa gcacggacga tgtgacagac ggaggagtgt gagcaattcg 1381 gatgacgatg ctggccggca gtcacgacaa agagtccgaa ggggcatgga cagggcaata 1441 tataaatgga aaagaggctt tgaatattcc taacccttcg tcgtcggcct cccaacttgc 1501 cctcgcatgc tgaattggcc gcctatctgc gaatggccgg ccgccgaccc taagtcgcaa 1561 taacttgaac ggagccgatg caaagtcctt atcgaacgct cgttattata aacggggcag 1621 acctaggtat ttaattatat caaaaccctc cgcaatttcg ctaatcattc cctaatttct 1681 tctactttgg cgtctagtgc tgtggtcgcg cttgctctca aagctcgctg ggaggcagtg 1741 gagcgtctgg cttaaaatcg atcgccgcaa ccctagagtt cctttcactt ctttctctct 1801 tgcgtggaga gcatcgacat cgaggagctg catcttgtct tctggtatgc gccttgtgcc 1861 ctcgtctctg tacgtcgtta cgattgctcg atattgtgat tgtctagacc tgaaacccga 1921 ctggagcttt tacgtggtgt cgattggggc ctcgagcgag tgatttttta ggttcgtttt 1981 agccaagtac atcccacttt ggtcagtcct gttatctcgg taaggtgttc agttcggtca 2041 ctggtgcgga ggtgcctcgt aagtttcctg catgatagat ttgtctatcc ttgacttgaa 2101 gaggagtcga aggtcagcga ggttggctgt gtgctatgga cgtcggtgct ccttggttcc 2161 gtgcttctat gggtgttgcc tcaacagtat tgatgtgcct gcagaaaggt tgtcaccaat 2221 ggggattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 2281 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt ttcggctgtc 2341 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 2401 ccaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 2461 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 2521 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 2581 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 2641 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 2701 agAAcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 2761 cggcgaggat ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa 2821 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 2881 catagcgttg gctacccgtg atattgctga agAActtggc ggcgaatggg ctgaccgctt 2941 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 3001 tgacgagttc ttctgagctt ctctagctag agtcgatcga caagctcgag tttctccata 3061 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt 3121 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa 3181 aatttctaat tcctaaaaCc aaaatccagt actaaaatcc agatcgctag catgaagagc 3241 aattctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat 3301 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 3361 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca 3421 gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa 3481 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt 3541 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc 3601 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa 3661 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc 3721 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg 3781 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag 3841 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc 3901 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc 3961 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt 4021 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag 4081 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac 4141 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc 4201 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag 4261 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga 4321 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg 4381 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca 4441 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa 4501 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca 4561 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac 4621 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga 4681 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac 4741 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga 4801 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc 4861 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga 4921 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt 4981 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc 5041 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac 5101 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt 5161 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa 5221 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct 5281 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg 5341 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag 5401 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag 5461 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc 5521 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc 5581 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc 5641 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg 5701 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg 5761 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg 5821 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc 5881 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg 5941 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag 6001 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg 6061 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc 6121 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga 6181 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg 6241 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg 6301 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag 6361 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt 6421 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc 6481 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc 6541 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac 6601 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc 6661 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta 6721 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg 6781 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag 6841 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat 6901 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca 6961 taactgtctg gccagcgcac agccgaagtg ctgcaaaaag cgcctaccct tcggtcgctg 7021 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg 7081 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac 7141 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 7201 ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag 7261 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 7321 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 7381 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 7441 atcaggcgct catccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 7501 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 7561 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7621 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7681 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 7741 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 7801 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 7861 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 7921 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 7981 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8041 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 8101 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 8161 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 8221 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 8281 gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg 8341 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga 8401 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat 8461 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc 8521 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat 8581 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc 8641 aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt 8701 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc 8761 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg 8821 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg 8881 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc 8941 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg 9001 gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc 9061 agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct 9121 cat //