LOCUS OP_062 9466 bp DNA circular 29-Apr-2025 ACCESSION OP_062 VERSION OP_062.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(3577..3583) /label=SapI recognition site oriT 7649..7789 /label=basis of mobility region from pBR322 rep_origin 7975..8563 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 4914..5543 /label=pVS1 StaA stability protein CDS 5972..7045 /label=pVS1 RepA replication protein rep_origin 7111..7305 /label=pVS1 oriV origin of replication misc_feature 4675..5675 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 3589..3613 /label=A.tumefaciens plasmid right boundary CDS complement(8651..9466) /label=nptI / KanR terminator 3364..3567 /label=3TERM_35S (MoClo) misc_feature 1211..1217 /label=TATA box promoter 1195..1240 /label=min 35S pro misc_feature 500..824 /label=35S misc_feature 825..1149 /label=35S promoter 500..1250 /label=p5-35Sx2 CDS 1323..3359 /label=mALS / CsR promoter 484..1289 /label=p5-35Sx2 rep_origin complement(7960..8574) /label=rep (pMB1) ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcaatgag 481 gaggctagag cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat 541 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc 601 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga 661 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga 721 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 781 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg 841 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg 901 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct 961 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg 1021 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc 1081 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg 1141 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag 1201 aCccttcctc tatataagga agttcatttc atttggagag gacacgctga aatcaccagt 1261 ctctctctac aaatctatct ctctcgagct ttcgcagatc cggggggcaa tgagatTCAC 1321 CAATGgcgtc aattagtgga tgcgcagctg tgcctttgag catagcgagc ctggagaagg 1381 tgcagccatc ggcaaggtct tgttgcaagg gagctaaaga gctaaaggtg aggagtggtt 1441 tcttctcggg agccgctctg aggggacatg catacaacac ctctcggaag actgagcgtt 1501 cactggaagt gaaagcccaa gctgttagcg agaagatcca gtctgcagtc gatgtgagtg 1561 gaccacggct tagcagcggg aagtcactcg ttgcaccaac cccgtccgac tttcagtctc 1621 gctacaagga tgacgagccc cgcaaaggag ccgacatttt ggtggaggct cttgagcgag 1681 aagggattag aacggtgttc gcttatcccg gcggagcgtc tatggagatt caccaggctc 1741 ttacacgttc cagcagcatc cgcaacattc tctgtcgcca cgagcagggg gagattttcg 1801 ctgctgaagg ttatgctaaa tcgtctggac gagtaggggt ttgtatagcc acatccggtc 1861 ctggagcgac caatcttgtt accgggctcg ctgatgccct cttggacagt gtgccactag 1921 tggccattac aggtcaagta tccagtagga tgattggtac cgatgctttt caagaaactc 1981 caattgtgga ggttacgaga tcgatcacca agcacaatta ccttgttatg aacattgatg 2041 acatccctcg cgtaatcagg gaggctttct ttttggcagc ttcaggtagg ccgggcccag 2101 tcttggtaga tattcccaag gacgtgcaac agcaaatggc tattccagat tggtcGcagc 2161 caatgcaact gaagggctac ttacaacgac tgcctcggcc accggaacca acgttaatcg 2221 agcagattat aagactcatt tctagttcta agaagcctgt agtgtattct ggaggaggtt 2281 gcaatcacgc cagcgaagag ttgagggaat ttgtcgagct taccggaatt cctatcacat 2341 cgactttaat gggtttggga gcgtatcccg agtctgatcc attaagtttg aaaatgcttg 2401 gaatgcatgg aactgtttac gccaactacg ccgtggacca agcagatctg ttgttggcat 2461 ttggagtgcg tttcgacgat cgagtgactg gtaaactgga gacttttgct agcaggtcca 2521 gcatcgttca tatcgatatt gaccctgctg aaattggaaa gaacaaacag ccccatgtgt 2581 ccatgtgtgc tgatgtgaaa cctgcgctaa gtgaaatcaa tgccctcttg aagagtcaaa 2641 agaggacttt cgacttctcc aactgggtgg cagaactaga agaacaaaag cagaaatggc 2701 caatgaagtt tcccgtattc gaggatgcca ttgctcctca atctgccata cagacgctgt 2761 acgaactcac tggtggaaat gccatcatat ctactggtgt tggccaacat caaatgtggg 2821 cagcccagtg gttcaagtac aacgaaccgc ggcgttggtt gacatctgga ggtcttggag 2881 ccatgggatt cggacttcct tctgctttgg gggctgccgc aactcaacca gatattcctg 2941 tgatcgacat cgatggcgat ggtagctttg tgatgaacgt acaggagttg gctacaattt 3001 ttgtggagaa gttacccgtg aagatcatga tcttaaacaa ccaacatcta ggaatggtcg 3061 tacaattgga ggatagattc tacaaggcca acagagcaca tacttactta ggtgatccag 3121 atcacgagga gcagatctac cccgacttct gtacagttgc agacggtttc aaggtgccaa 3181 atgcgcgtgt ggtgaagaag gatgaactaa gagcagcaat tcaaactatg ctagattctc 3241 ccggtcctta tttactggat gtgatagttc cccaccagga gcacgttctg cctatgattc 3301 ctgggggagc aagttttaag gagattatca cagagggtga tggaaggact atatactaaG 3361 CTTctctagc tagagtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt 3421 cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa 3481 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa 3541 aCcaaaatcc agtactaaaa tccagatcgc tagcatgaag agcaattctg acaggatata 3601 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc 3661 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg 3721 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc 3781 agtgcagccg tcatctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc 3841 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac 3901 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg 3961 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc 4021 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga 4081 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga 4141 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg 4201 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat 4261 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac 4321 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct 4381 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa 4441 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag 4501 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac 4561 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg 4621 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc 4681 gcggccgggt acgtgttcga gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc 4741 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa 4801 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg 4861 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg 4921 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag 4981 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca 5041 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc 5101 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg 5161 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga 5221 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta 5281 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga 5341 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca 5401 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa 5461 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg 5521 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa 5581 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct 5641 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa 5701 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat 5761 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta 5821 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt 5881 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg 5941 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt 6001 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc 6061 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga 6121 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc 6181 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg 6241 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc 6301 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc 6361 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga 6421 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca 6481 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct 6541 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc 6601 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat 6661 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta 6721 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt 6781 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc 6841 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa 6901 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt 6961 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga 7021 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc 7081 aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc acgtacattg ggaacccaaa 7141 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg 7201 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc 7261 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg 7321 cacagccgaa gtgctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc 7381 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg 7441 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca 7501 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 7561 cggtgacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 7621 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 7681 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 7741 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcatccgc 7801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 8041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 8101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 8161 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 8221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 8281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 8341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 8401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca 8641 ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag 8701 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga 8761 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat 8821 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat 8881 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt 8941 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca 9001 ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga caattacaaa 9061 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg 9121 aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca gtggtgagta 9181 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg 9241 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat 9301 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt gtcgcacctg 9361 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat 9421 ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcat //