LOCUS OP_063 8479 bp DNA circular 29-Apr-2025 ACCESSION OP_063 VERSION OP_063.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(2590..2596) /label=SapI recognition site oriT 6662..6802 /label=basis of mobility region from pBR322 rep_origin 6988..7576 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 3927..4556 /label=pVS1 StaA stability protein CDS 4985..6058 /label=pVS1 RepA replication protein rep_origin 6124..6318 /label=pVS1 oriV origin of replication misc_feature 3688..4688 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 2602..2626 /label=A.tumefaciens plasmid right boundary CDS complement(7664..8479) /label=nptI / KanR terminator 2377..2580 /label=3TERM_35S (MoClo) misc_feature 1283..1289 /label=TATA box promoter 1267..1312 /label=min 35S pro CDS 1347..2372 /label=hptII / HygR promoter 484..1318 /label=p5-35S rep_origin complement(6973..7587) /label=rep (pMB1) ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcaatgag 481 gagAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT 541 ACGCAGCAAG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTACAGGAA ATCAAATACC 601 TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG 661 AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC 721 TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT 781 CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG 841 GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA 901 TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGTAGACC 961 AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT 1021 GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT 1081 GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA 1141 AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AAACGTTCCA ACCACGTCTA 1201 CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT 1261 ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG 1321 GGACTCTAGA GGATCCAACA TCACCAATGa aaaagcctga actcaccgcg acgtctgtcg 1381 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg 1441 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata 1501 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc 1561 tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct 1621 cccgccgttc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc 1681 tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg 1741 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat 1801 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg 1861 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc 1921 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa 1981 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca 2041 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga 2101 ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg 2161 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc 2221 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca 2281 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac 2341 gccccagcac tcgtccgagg gcaaagaaat agGCTTctct agctagagtc gatcgacaag 2401 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat 2461 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa 2521 atacttctat caataaaatt tctaattcct aaaaCcaaaa tccagtacta aaatccagat 2581 cgctagcatg aagagcaatt ctgacaggat atattggcgg gtaaacctaa gagaaaagag 2641 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt 2701 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct 2761 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat 2821 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct 2881 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc 2941 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca 3001 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga 3061 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg 3121 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct 3181 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc 3241 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc 3301 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc 3361 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg 3421 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca 3481 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag 3541 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga 3601 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt 3661 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc 3721 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg 3781 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga 3841 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta 3901 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg 3961 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg 4021 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg 4081 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg 4141 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg 4201 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca 4261 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa 4321 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg 4381 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg 4441 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg 4501 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta 4561 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc 4621 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt 4681 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg 4741 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa 4801 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac 4861 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg 4921 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat 4981 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 5041 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 5101 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 5161 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 5221 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 5281 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 5341 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 5401 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 5461 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 5521 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 5581 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 5641 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 5701 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 5761 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 5821 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 5881 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 5941 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 6001 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 6061 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt 6121 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 6181 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 6241 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 6301 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagtgctgc aaaaagcgcc 6361 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 6421 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 6481 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 6541 atgacggtga aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag 6601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 6661 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 6721 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 6781 aaggagaaaa taccgcatca ggcgctcatc cgcttcctcg ctcactgact cgctgcgctc 6841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 6901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 7021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 7081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 7141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 7201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 7261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 7321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 7381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 7441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 7501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 7621 cgaaaactca cgttaaggga ttttggtcat gcattctagg tgattagaaa aactcatcga 7681 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa 7741 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct 7801 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt 7861 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg 7921 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat 7981 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa 8041 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga 8101 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga 8161 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa 8221 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat 8281 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg 8341 gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt 8401 tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt 8461 cccgttgaat atggctcat //