LOCUS OP_066 10873 bp DNA circular 29-Apr-2025 ACCESSION OP_066 VERSION OP_066.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(4984..4990) /label=SapI recognition site oriT 9056..9196 /label=basis of mobility region from pBR322 rep_origin 9382..9970 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 6321..6950 /label=pVS1 StaA stability protein CDS 7379..8452 /label=pVS1 RepA replication protein rep_origin 8518..8712 /label=pVS1 oriV origin of replication misc_feature 6082..7082 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 4996..5020 /label=A.tumefaciens plasmid right boundary CDS complement(10058..10873) /label=nptI / KanR misc_feature 4502..4755 /label=nosT (MoClo) terminator 4771..4974 /label=35ST (MoClo) misc_feature 2280..3051 /label=exon 5UTR_MpUBE2 Mapoly0106s0019 misc_feature 2280..3505 /label=5UTR_MpUBE2 Mapoly0106s0019 misc_feature 3052..3272 /label=intron in 5UTR_MpUBE2 promoter 484..2275 /label=PROM_MpUBE2 Mapoly0106s0019 misc_feature 3273..3505 /label=exon 5UTR_MpUBE2 Mapoly0106s0019 CDS 3507..4223 /label=mTurquoise2 no Stop misc_feature 4254..4481 /label=part of AT4G19150 CDS 4242..4481 /label=N7-NLS misc_feature 4227..4241 /label=S4xAla linker rep_origin complement(9367..9981) /label=rep (pMB1) terminator 4502..4974 /label=3TERM_Nos-35S CDS 3510..4223 /label=mTurquoise2 no ATG no STOP ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcatacag 481 gagacggggc ggataaaaat tttggcggtg tttccagccc tccaaccact aaatgcgaaa 541 ccgcccaacc caaaacctcc ttgcggatag atctcgggta cagtgtttac cgtgcggggg 601 gaaacgtgca tggcattatt ccgacagcct gaactcaggt tgggggccga gcggggagaa 661 ggccgcggtg cggtcatgac aagggaatcg agtcgcagga cgtggaagcc caaagtcggc 721 gacccctggc ttcggaaatc attgcgtaat ccggacttcc ctgcaaggcc cggcacggcc 781 atgatggcac atgggtcatt cggtgggccg ggggtggtca ccggactcga acgtgagaat 841 agcatttctg gccataggct cgaaccaggc gatcgtaggt gtggacatgc taccggcagc 901 gggatgggga ccgtcgaaac cgggcggacg gaccggcagg tgcggcagtc ggggcccgaa 961 agaggacgac gagggccggg aagaactggg acgacggggg tgacgcgtcg cgtagcgtgg 1021 agaagcaagg ccccgagcga cgggacgacg aggccggacg tgagtctctg cagtcctccg 1081 caaggcacga acgaagggaa cgacgagcgc tgaaagggtc gaacaagtgg actgtcgctg 1141 cggccaggat cgacggggac cggggctagc tagatcgggg cgactgaccg actgaccctc 1201 tgaatgtccg tccatctgga agggaatttc ggagcaggat taaatagcta cacgacccag 1261 gactgtggcc ccagagcgtg ccgagcctca gcgccccact cacgagggcg aaaccaaacg 1321 catggctccc gtcggcccag ctgtgccgcg tcggacactt cgtcattgat tatccgcccg 1381 tcgggccgat ggcgcgaccg gaacagcatt taccagattg ttccagacag cctcggggat 1441 ttaggtctag tagctgtccg agcttggcgt gcagcttcgg ttcggtgccc ggaagttacc 1501 agcaccaaaa cgcgtggaac ttgtagcctg gaggtagctg gcgtagcggc ttacttgttg 1561 cggatagaag agagtcccgc aggagatcgg gtggagtgcc accggaccgg gtgttccggt 1621 ccccattgag gacagaacgg tagctggctc ggatgtgtgg ataaagttgc cgtcgcctgt 1681 cgcgatggat ggatggatgg aaggatggat ggagccagtg attctgggtg ccgagtgcgg 1741 atgtgtcacg gggaaggctg cgaggaacgg caaaacgtgc ctcgaccgtg taacatgacg 1801 caaacggcag cggggcggca tgccatccgc cggggccatg ccgcgtggcc gtcggattcg 1861 agatccaccg agccggagag cccgaagcac tgacggatga agtccacgcc agaaactcgg 1921 gggacgtacg tggtatcgaa ggccgaggag gaggacagag agcggaatcg ggacataacc 1981 gggccaaccg tccgatcccg aatccgaagg tggagggact ttgcgtccgg agattcagag 2041 ccgtgaaacg cgaaatgcag cgtcagacgc cggaagcttc gtcgcgctgg atggcgggcg 2101 agaagagggt tctgatttga ccggcggatg cggatgcggg tgcggatccg acagtcgaat 2161 gggaagggag gactgcaatt cacggccacg accgggagca ggactgactt tgaactggta 2221 cggagcgagg gtgtgcgggc ggagcatatt ccgtccacat cgaagtcggg gtcggtactg 2281 ttagggaggt ggggtggggt tgggtcgggt cgggccgcgt gatgagatgg ctatggcggt 2341 cgtcgtcgtc gtcgtcgtcc gtgctgcctg tgctgctgct gctcctcctc cttgcgctgc 2401 cgtcccgggc tgcctcgctc gcctgcctcg ccctcctcct cctcctcgcc ctcgtcctcg 2461 ctgcctgccc ttgctggctt tgcttatgag attgtgagaa ataggcaggc tggccgggct 2521 ggttggcgat tgactctctg caacgctcct cccctcccct tgccttcagg acagggttgg 2581 tgattctctt ctggatcttt gctctcgctg cttgcgttgc cttgcgttgc gttgctcgcc 2641 cgtgttcttg aattgggatt gctagtgctc cggtgtttga tttcgtgtct cgtcgtcgtc 2701 ctcgcggtcc ctgtcgctgt gagtggtgcg tgcgtgactg ttgttggttc gcgtgtgcac 2761 ttgtttgggt ggagtttagc gcaagagagg agggaaggaa gagtgttcga agagaggtgc 2821 cgggcgacgg tgggaatcag gcaccgctat ctggccgagg cagaggcaaa ggcagaggca 2881 ggcaggcgga gacgtagaga gcgggggcga gagaagggaa ggaagagaga gagagagaga 2941 gagagagaga gagagagaga gagagaggga gaggttgcct atagagtcgg ggagatctgt 3001 gttaggcttc ttcttggtga agtgtatgtt ggcagcagcg tcgtcggcag ggtaccttcc 3061 cgttctctca cgccgccgct ttcgtcctct tgccacgccc tgcccgctgc gggctgggcc 3121 gagtcgtcct ggcccctgtg ttgtccaccg agttctaggg ctttcctgcg tgcgcgtgcg 3181 atagttcgat gcccttctct ttgaggtttc ttttgccgcc actcgactga tcatgttttg 3241 tgcatatatc gtgggcctgc tctttgcttc agggtttcct tcaagcttgc tatcaacgac 3301 ggaccttcgg ctttcctgct agtggagcgt atcgacccgt taatttgacg tggtttttca 3361 tcatcgcgct tcgaggatct ctgtgactgg catcggggaa ccaaagacaa ctgatttgat 3421 cgaacttaga agtttgtgtt cagagaaaca atctaaagag gtagattcga gagcagttgg 3481 gaagcgcttg gcggtgctgg tgcccaatgg tgagcaaggg cgaggagctg ttcaccgggg 3541 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 3601 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 3661 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct 3721 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 3781 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 3841 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 3901 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctactttagc gacaacgtct 3961 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca 4021 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 4081 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc 4141 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 4201 tcggcatgga cgagctgtac aaggcttcgg ctgcagcggc cgaattcaag cgtgaagaac 4261 aagcaaggaa agctaaggtg aacaatgaga aaaagacgga aatagtgaaa ccagagagtt 4321 gtagcaatga aggagatgtc aaggatctga aaagaaagga ctctgaggat ggaaacgagg 4381 gtgaggaaga agaagcttct tcgaaaccga aaaagccaaa agttgctctt tctcatcttc 4441 aggacattga cgacacagaa gctgatcaag aagaagagta agcttgagct cgaatttccc 4501 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc 4561 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg 4621 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata 4681 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc 4741 tatgttacta gatcGATCCG TATCGATAGC ctctagctag agtcgatcga caagctcgag 4801 tttctccata ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt 4861 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt 4921 ctatcaataa aatttctaat tcctaaaaTc aaaatccagt actaaaatcc agatCGCTac 4981 agtgaagagc aattctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta 5041 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 5101 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg 5161 ctatagtgca gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca 5221 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc 5281 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa 5341 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt 5401 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat 5461 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg 5521 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga 5581 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc 5641 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 5701 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 5761 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 5821 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 5881 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 5941 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 6001 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 6061 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 6121 ctctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 6181 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 6241 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 6301 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 6361 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 6421 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 6481 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 6541 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 6601 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 6661 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 6721 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 6781 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 6841 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 6901 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 6961 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 7021 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 7081 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 7141 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 7201 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 7261 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 7321 gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 7381 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 7441 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 7501 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 7561 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 7621 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 7681 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 7741 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 7801 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 7861 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 7921 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 7981 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 8041 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 8101 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 8161 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 8221 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 8281 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 8341 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 8401 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 8461 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt 8521 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 8581 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 8641 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 8701 ggcctgtgca taactgtctg gccagcgcac agccgaagtg ctgcaaaaag cgcctaccct 8761 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 8821 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 8881 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 8941 gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg 9001 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 9061 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 9121 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9181 aaaataccgc atcaggcgct catccgcttc ctcgctcact gactcgctgc gctcggtcgt 9241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 9301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 9361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 9421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 9481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 9541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 9601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 9661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 9721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 9781 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 9841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 9901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 9961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 10021 ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca 10081 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt 10141 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc 10201 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa 10261 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa 10321 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat 10381 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc 10441 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg 10501 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg 10561 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct 10621 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa 10681 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc 10741 catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc 10801 catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt 10861 gaatatggct cat //