LOCUS OP_067 10840 bp DNA circular 29-Apr-2025 ACCESSION OP_067 VERSION OP_067.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(4951..4957) /label=SapI recognition site oriT 9023..9163 /label=basis of mobility region from pBR322 rep_origin 9349..9937 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 6288..6917 /label=pVS1 StaA stability protein CDS 7346..8419 /label=pVS1 RepA replication protein rep_origin 8485..8679 /label=pVS1 oriV origin of replication misc_feature 6049..7049 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 4963..4987 /label=A.tumefaciens plasmid right boundary CDS complement(10025..10840) /label=nptI / KanR misc_feature 4469..4722 /label=nosT (MoClo) terminator 4738..4941 /label=35ST (MoClo) misc_feature 2280..3051 /label=exon 5UTR_MpUBE2 Mapoly0106s0019 misc_feature 2280..3505 /label=5UTR_MpUBE2 Mapoly0106s0019 misc_feature 3052..3272 /label=intron in 5UTR_MpUBE2 promoter 484..2275 /label=PROM_MpUBE2 Mapoly0106s0019 misc_feature 3273..3505 /label=exon 5UTR_MpUBE2 Mapoly0106s0019 CDS 4281..4445 /label=AtRCI2B AT3G05890 misc_feature 4227..4262 /label=SG10xAla linker CDS 4263..4445 /label=Lti6b CDS 3507..4223 /label=eGFP no Stop CDS 3510..4223 /label=eGFP no ATG no Stop rep_origin complement(9334..9948) /label=rep (pMB1) terminator 4469..4941 /label=3TERM_Nos-35S misc_feature 4230..4448 /label=CTAG_linker-Lti6b ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcatacag 481 gagacggggc ggataaaaat tttggcggtg tttccagccc tccaaccact aaatgcgaaa 541 ccgcccaacc caaaacctcc ttgcggatag atctcgggta cagtgtttac cgtgcggggg 601 gaaacgtgca tggcattatt ccgacagcct gaactcaggt tgggggccga gcggggagaa 661 ggccgcggtg cggtcatgac aagggaatcg agtcgcagga cgtggaagcc caaagtcggc 721 gacccctggc ttcggaaatc attgcgtaat ccggacttcc ctgcaaggcc cggcacggcc 781 atgatggcac atgggtcatt cggtgggccg ggggtggtca ccggactcga acgtgagaat 841 agcatttctg gccataggct cgaaccaggc gatcgtaggt gtggacatgc taccggcagc 901 gggatgggga ccgtcgaaac cgggcggacg gaccggcagg tgcggcagtc ggggcccgaa 961 agaggacgac gagggccggg aagaactggg acgacggggg tgacgcgtcg cgtagcgtgg 1021 agaagcaagg ccccgagcga cgggacgacg aggccggacg tgagtctctg cagtcctccg 1081 caaggcacga acgaagggaa cgacgagcgc tgaaagggtc gaacaagtgg actgtcgctg 1141 cggccaggat cgacggggac cggggctagc tagatcgggg cgactgaccg actgaccctc 1201 tgaatgtccg tccatctgga agggaatttc ggagcaggat taaatagcta cacgacccag 1261 gactgtggcc ccagagcgtg ccgagcctca gcgccccact cacgagggcg aaaccaaacg 1321 catggctccc gtcggcccag ctgtgccgcg tcggacactt cgtcattgat tatccgcccg 1381 tcgggccgat ggcgcgaccg gaacagcatt taccagattg ttccagacag cctcggggat 1441 ttaggtctag tagctgtccg agcttggcgt gcagcttcgg ttcggtgccc ggaagttacc 1501 agcaccaaaa cgcgtggaac ttgtagcctg gaggtagctg gcgtagcggc ttacttgttg 1561 cggatagaag agagtcccgc aggagatcgg gtggagtgcc accggaccgg gtgttccggt 1621 ccccattgag gacagaacgg tagctggctc ggatgtgtgg ataaagttgc cgtcgcctgt 1681 cgcgatggat ggatggatgg aaggatggat ggagccagtg attctgggtg ccgagtgcgg 1741 atgtgtcacg gggaaggctg cgaggaacgg caaaacgtgc ctcgaccgtg taacatgacg 1801 caaacggcag cggggcggca tgccatccgc cggggccatg ccgcgtggcc gtcggattcg 1861 agatccaccg agccggagag cccgaagcac tgacggatga agtccacgcc agaaactcgg 1921 gggacgtacg tggtatcgaa ggccgaggag gaggacagag agcggaatcg ggacataacc 1981 gggccaaccg tccgatcccg aatccgaagg tggagggact ttgcgtccgg agattcagag 2041 ccgtgaaacg cgaaatgcag cgtcagacgc cggaagcttc gtcgcgctgg atggcgggcg 2101 agaagagggt tctgatttga ccggcggatg cggatgcggg tgcggatccg acagtcgaat 2161 gggaagggag gactgcaatt cacggccacg accgggagca ggactgactt tgaactggta 2221 cggagcgagg gtgtgcgggc ggagcatatt ccgtccacat cgaagtcggg gtcggtactg 2281 ttagggaggt ggggtggggt tgggtcgggt cgggccgcgt gatgagatgg ctatggcggt 2341 cgtcgtcgtc gtcgtcgtcc gtgctgcctg tgctgctgct gctcctcctc cttgcgctgc 2401 cgtcccgggc tgcctcgctc gcctgcctcg ccctcctcct cctcctcgcc ctcgtcctcg 2461 ctgcctgccc ttgctggctt tgcttatgag attgtgagaa ataggcaggc tggccgggct 2521 ggttggcgat tgactctctg caacgctcct cccctcccct tgccttcagg acagggttgg 2581 tgattctctt ctggatcttt gctctcgctg cttgcgttgc cttgcgttgc gttgctcgcc 2641 cgtgttcttg aattgggatt gctagtgctc cggtgtttga tttcgtgtct cgtcgtcgtc 2701 ctcgcggtcc ctgtcgctgt gagtggtgcg tgcgtgactg ttgttggttc gcgtgtgcac 2761 ttgtttgggt ggagtttagc gcaagagagg agggaaggaa gagtgttcga agagaggtgc 2821 cgggcgacgg tgggaatcag gcaccgctat ctggccgagg cagaggcaaa ggcagaggca 2881 ggcaggcgga gacgtagaga gcgggggcga gagaagggaa ggaagagaga gagagagaga 2941 gagagagaga gagagagaga gagagaggga gaggttgcct atagagtcgg ggagatctgt 3001 gttaggcttc ttcttggtga agtgtatgtt ggcagcagcg tcgtcggcag ggtaccttcc 3061 cgttctctca cgccgccgct ttcgtcctct tgccacgccc tgcccgctgc gggctgggcc 3121 gagtcgtcct ggcccctgtg ttgtccaccg agttctaggg ctttcctgcg tgcgcgtgcg 3181 atagttcgat gcccttctct ttgaggtttc ttttgccgcc actcgactga tcatgttttg 3241 tgcatatatc gtgggcctgc tctttgcttc agggtttcct tcaagcttgc tatcaacgac 3301 ggaccttcgg ctttcctgct agtggagcgt atcgacccgt taatttgacg tggtttttca 3361 tcatcgcgct tcgaggatct ctgtgactgg catcggggaa ccaaagacaa ctgatttgat 3421 cgaacttaga agtttgtgtt cagagaaaca atctaaagag gtagattcga gagcagttgg 3481 gaagcgcttg gcggtgctgg tgcccaatgg tgagcaaggg cgaggagctg ttcaccgggg 3541 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 3601 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 3661 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 3721 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 3781 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 3841 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 3901 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 3961 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 4021 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 4081 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 4141 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 4201 tcggcatgga cgagctgtac aaggcttcgg gagctgcggc agctgccgct gcggcagcgg 4261 ccgaattcaa gcgcttgaaa atgagtacag ccactttcgt agagattatt cttgctatca 4321 tcttgcctcc tctcggcgtc tttctcaaat ttggttgcaa ggttgagttt tggatatgtt 4381 tgattttgac gctgtttggt tatcttcccg gaatccttta cgctctttat atcatcacct 4441 tttgatgagc ttgagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt 4501 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt 4561 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat 4621 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta 4681 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cGATCCGTAT CGATAGCctc 4741 tagctagagt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga 4801 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta 4861 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaTcaaa 4921 atccagtact aaaatccaga tCGCTacagt gaagagcaat tctgacagga tatattggcg 4981 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa 5041 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca 5101 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca 5161 gccgtcatct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct 5221 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga 5281 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc 5341 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg 5401 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc 5461 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct 5521 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg 5581 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt 5641 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag 5701 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc 5761 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc 5821 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga 5881 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc 5941 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag 6001 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc 6061 gggtacgtgt tcgagccgcc cgcgcacctc tcaaccgtgc ggctgcatga aatcctggcc 6121 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag 6181 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct 6241 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg 6301 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg 6361 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc 6421 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga 6481 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc 6541 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg 6601 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc 6661 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag 6721 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg 6781 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc 6841 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta 6901 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct 6961 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga 7021 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa 7081 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca 7141 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag 7201 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag 7261 gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg 7321 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat 7381 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg 7441 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat 7501 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa 7561 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag 7621 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga 7681 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat 7741 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat 7801 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc 7861 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga 7921 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa 7981 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa 8041 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga 8101 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga 8161 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc 8221 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt 8281 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga 8341 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga 8401 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga 8461 aaaaggtcga aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta 8521 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca 8581 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa 8641 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc 8701 cgaagtgctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc 8761 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct 8821 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac 8881 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga 8941 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 9001 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 9061 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 9121 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctcat ccgcttcctc 9181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 9241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 9301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 9361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 9421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 9481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 9541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 9601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 9661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 9721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 9781 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 9841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 9901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 9961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag 10021 gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat 10081 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt 10141 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac 10201 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga 10261 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag 10321 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg 10381 attgcgcctg agcgagtcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa 10441 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag 10501 gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg 10561 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc 10621 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca 10681 gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc 10741 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc 10801 gcggccttga gcaagacgtt tcccgttgaa tatggctcat //