LOCUS OP_070 10819 bp DNA circular 29-Apr-2025 ACCESSION OP_070 VERSION OP_070.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(4930..4936) /label=SapI recognition site oriT 9002..9142 /label=basis of mobility region from pBR322 rep_origin 9328..9916 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 6267..6896 /label=pVS1 StaA stability protein CDS 7325..8398 /label=pVS1 RepA replication protein rep_origin 8464..8658 /label=pVS1 oriV origin of replication misc_feature 6028..7028 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 4942..4966 /label=A.tumefaciens plasmid right boundary CDS complement(10004..10819) /label=nptI / KanR misc_feature 4448..4701 /label=nosT (MoClo) terminator 4717..4920 /label=35ST (MoClo) misc_feature 2280..3051 /label=exon 5UTR_MpUBE2 Mapoly0106s0019 misc_feature 2280..3505 /label=5UTR_MpUBE2 Mapoly0106s0019 misc_feature 3052..3272 /label=intron in 5UTR_MpUBE2 promoter 484..2275 /label=PROM_MpUBE2 Mapoly0106s0019 misc_feature 3273..3505 /label=exon 5UTR_MpUBE2 Mapoly0106s0019 CDS 4260..4424 /label=AtRCI2B AT3G05890 misc_feature 4206..4241 /label=SG10xAla linker CDS 4242..4424 /label=Lti6b CDS 3507..4202 /label=mScarlet-I no Stop rep_origin complement(9313..9927) /label=rep (pMB1) terminator 4448..4920 /label=3TERM_Nos-35S misc_feature 4209..4427 /label=CTAG_linker-Lti6b ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcacagag 481 gagacggggc ggataaaaat tttggcggtg tttccagccc tccaaccact aaatgcgaaa 541 ccgcccaacc caaaacctcc ttgcggatag atctcgggta cagtgtttac cgtgcggggg 601 gaaacgtgca tggcattatt ccgacagcct gaactcaggt tgggggccga gcggggagaa 661 ggccgcggtg cggtcatgac aagggaatcg agtcgcagga cgtggaagcc caaagtcggc 721 gacccctggc ttcggaaatc attgcgtaat ccggacttcc ctgcaaggcc cggcacggcc 781 atgatggcac atgggtcatt cggtgggccg ggggtggtca ccggactcga acgtgagaat 841 agcatttctg gccataggct cgaaccaggc gatcgtaggt gtggacatgc taccggcagc 901 gggatgggga ccgtcgaaac cgggcggacg gaccggcagg tgcggcagtc ggggcccgaa 961 agaggacgac gagggccggg aagaactggg acgacggggg tgacgcgtcg cgtagcgtgg 1021 agaagcaagg ccccgagcga cgggacgacg aggccggacg tgagtctctg cagtcctccg 1081 caaggcacga acgaagggaa cgacgagcgc tgaaagggtc gaacaagtgg actgtcgctg 1141 cggccaggat cgacggggac cggggctagc tagatcgggg cgactgaccg actgaccctc 1201 tgaatgtccg tccatctgga agggaatttc ggagcaggat taaatagcta cacgacccag 1261 gactgtggcc ccagagcgtg ccgagcctca gcgccccact cacgagggcg aaaccaaacg 1321 catggctccc gtcggcccag ctgtgccgcg tcggacactt cgtcattgat tatccgcccg 1381 tcgggccgat ggcgcgaccg gaacagcatt taccagattg ttccagacag cctcggggat 1441 ttaggtctag tagctgtccg agcttggcgt gcagcttcgg ttcggtgccc ggaagttacc 1501 agcaccaaaa cgcgtggaac ttgtagcctg gaggtagctg gcgtagcggc ttacttgttg 1561 cggatagaag agagtcccgc aggagatcgg gtggagtgcc accggaccgg gtgttccggt 1621 ccccattgag gacagaacgg tagctggctc ggatgtgtgg ataaagttgc cgtcgcctgt 1681 cgcgatggat ggatggatgg aaggatggat ggagccagtg attctgggtg ccgagtgcgg 1741 atgtgtcacg gggaaggctg cgaggaacgg caaaacgtgc ctcgaccgtg taacatgacg 1801 caaacggcag cggggcggca tgccatccgc cggggccatg ccgcgtggcc gtcggattcg 1861 agatccaccg agccggagag cccgaagcac tgacggatga agtccacgcc agaaactcgg 1921 gggacgtacg tggtatcgaa ggccgaggag gaggacagag agcggaatcg ggacataacc 1981 gggccaaccg tccgatcccg aatccgaagg tggagggact ttgcgtccgg agattcagag 2041 ccgtgaaacg cgaaatgcag cgtcagacgc cggaagcttc gtcgcgctgg atggcgggcg 2101 agaagagggt tctgatttga ccggcggatg cggatgcggg tgcggatccg acagtcgaat 2161 gggaagggag gactgcaatt cacggccacg accgggagca ggactgactt tgaactggta 2221 cggagcgagg gtgtgcgggc ggagcatatt ccgtccacat cgaagtcggg gtcggtactg 2281 ttagggaggt ggggtggggt tgggtcgggt cgggccgcgt gatgagatgg ctatggcggt 2341 cgtcgtcgtc gtcgtcgtcc gtgctgcctg tgctgctgct gctcctcctc cttgcgctgc 2401 cgtcccgggc tgcctcgctc gcctgcctcg ccctcctcct cctcctcgcc ctcgtcctcg 2461 ctgcctgccc ttgctggctt tgcttatgag attgtgagaa ataggcaggc tggccgggct 2521 ggttggcgat tgactctctg caacgctcct cccctcccct tgccttcagg acagggttgg 2581 tgattctctt ctggatcttt gctctcgctg cttgcgttgc cttgcgttgc gttgctcgcc 2641 cgtgttcttg aattgggatt gctagtgctc cggtgtttga tttcgtgtct cgtcgtcgtc 2701 ctcgcggtcc ctgtcgctgt gagtggtgcg tgcgtgactg ttgttggttc gcgtgtgcac 2761 ttgtttgggt ggagtttagc gcaagagagg agggaaggaa gagtgttcga agagaggtgc 2821 cgggcgacgg tgggaatcag gcaccgctat ctggccgagg cagaggcaaa ggcagaggca 2881 ggcaggcgga gacgtagaga gcgggggcga gagaagggaa ggaagagaga gagagagaga 2941 gagagagaga gagagagaga gagagaggga gaggttgcct atagagtcgg ggagatctgt 3001 gttaggcttc ttcttggtga agtgtatgtt ggcagcagcg tcgtcggcag ggtaccttcc 3061 cgttctctca cgccgccgct ttcgtcctct tgccacgccc tgcccgctgc gggctgggcc 3121 gagtcgtcct ggcccctgtg ttgtccaccg agttctaggg ctttcctgcg tgcgcgtgcg 3181 atagttcgat gcccttctct ttgaggtttc ttttgccgcc actcgactga tcatgttttg 3241 tgcatatatc gtgggcctgc tctttgcttc agggtttcct tcaagcttgc tatcaacgac 3301 ggaccttcgg ctttcctgct agtggagcgt atcgacccgt taatttgacg tggtttttca 3361 tcatcgcgct tcgaggatct ctgtgactgg catcggggaa ccaaagacaa ctgatttgat 3421 cgaacttaga agtttgtgtt cagagaaaca atctaaagag gtagattcga gagcagttgg 3481 gaagcgcttg gcggtgctgg tgcccaatgg tgagcaaggg cgaggcagtg atcaaggagt 3541 tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc gagatcgagg 3601 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 3661 gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac ggctccaggg 3721 ccttcatcaa gcaccccgcc gacatccccg actactataa gcagtccttc cccgagggct 3781 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg acccaggaca 3841 cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc aacttccctc 3901 ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcgtccacc gagcggttgt 3961 accccgagga cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg aaggacggcg 4021 gccgctacct ggcggacttc aagaccacct acaaggccaa gaagcccgtg cagatgcccg 4081 gcgcctacaa cgtcgaccgc aagttggaca tcacctccca caacgaggac tacaccgtgg 4141 tggaacagta cgaacgctcc gagggccgcc actccaccgg cggcatggac gagctgtaca 4201 aggcttcggg agctgcggca gctgccgctg cggcagcggc cgaattcaag cgcttgaaaa 4261 tgagtacagc cactttcgta gagattattc ttgctatcat cttgcctcct ctcggcgtct 4321 ttctcaaatt tggttgcaag gttgagtttt ggatatgttt gattttgacg ctgtttggtt 4381 atcttcccgg aatcctttac gctctttata tcatcacctt ttgatgagct tgagctcgaa 4441 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg 4501 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat 4561 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat 4621 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt 4681 gtcatctatg ttactagatc GATCCGTATC GATAGCctct agctagagtc gatcgacaag 4741 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat 4801 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa 4861 atacttctat caataaaatt tctaattcct aaaaTcaaaa tccagtacta aaatccagat 4921 CGCTaggttg aagagcaatt ctgacaggat atattggcgg gtaaacctaa gagaaaagag 4981 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt 5041 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct 5101 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcatctg aaaacgacat 5161 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct 5221 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc 5281 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca 5341 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga 5401 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg 5461 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct 5521 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc 5581 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc 5641 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc 5701 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg 5761 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca 5821 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag 5881 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga 5941 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt 6001 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc 6061 gcgcacctct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg 6121 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga 6181 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta 6241 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg 6301 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg 6361 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg 6421 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg 6481 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg 6541 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca 6601 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa 6661 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg 6721 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg 6781 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg 6841 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta 6901 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc 6961 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt 7021 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg 7081 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa 7141 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac 7201 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg 7261 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat 7321 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga 7381 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc 7441 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc 7501 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt 7561 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc 7621 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc 7681 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga 7741 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa 7801 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg 7861 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac 7921 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc 7981 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga 8041 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc 8101 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct 8161 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat 8221 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct 8281 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc 8341 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg 8401 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggactctt 8461 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta 8521 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa 8581 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac 8641 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagtgctgc aaaaagcgcc 8701 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc 8761 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc 8821 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg 8881 atgacggtga aaacctctga cacatgcagc tcccggtgac ggtcacagct tgtctgtaag 8941 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 9001 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 9061 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 9121 aaggagaaaa taccgcatca ggcgctcatc cgcttcctcg ctcactgact cgctgcgctc 9181 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 9241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 9301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 9361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 9421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 9481 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 9541 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 9601 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 9661 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9721 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 9781 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 9841 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 9901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9961 cgaaaactca cgttaaggga ttttggtcat gcattctagg tgattagaaa aactcatcga 10021 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa 10081 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct 10141 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt 10201 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg 10261 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat 10321 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa 10381 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga 10441 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga 10501 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa 10561 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat 10621 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg 10681 gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt 10741 tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt 10801 cccgttgaat atggctcat //