LOCUS OP_075 9098 bp DNA circular 29-Apr-2025 ACCESSION OP_075 VERSION OP_075.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(3209..3215) /label=SapI recognition site oriT 7281..7421 /label=basis of mobility region from pBR322 rep_origin 7607..8195 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 4546..5175 /label=pVS1 StaA stability protein CDS 5604..6677 /label=pVS1 RepA replication protein rep_origin 6743..6937 /label=pVS1 oriV origin of replication misc_feature 4307..5307 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 3221..3245 /label=A.tumefaciens plasmid right boundary CDS complement(8283..9098) /label=nptI / KanR promoter complement(2960..2989) /label=lac promoter misc_feature complement(2933..2953) /label=lac operator CDS complement(2594..2915) /label=LacZa promoter 484..2495 /label=p5-MpU6 Sugano rep_origin complement(7592..8206) /label=rep (pMB1) ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcagcaag 481 gagTATTCAT TCAAAAGAGA TTTTTAAAGA TCAATTTTTC TTTCTCATAA GTCTAATACG 541 TCATGATAAT AACTTTAAAA AAAATTGAAC TCGTTAAAAA ATTTGATCAA ATTTTAATTT 601 ATTATTAGAT CATATATTAC ATATCAGTTT CATAATGTTA TACTAAGATC TTTCATATAT 661 ATTTTAACCC ACGAGACAAA ATATGTTTAT ATCTTTATTT ATTTAAATAT TTTTTAGCTC 721 AAAACATCGC AAACTCAGCT ATCATAATGC TTTTTAATGA AAAAAACAAA TTTTCCTATC 781 ATACTCTCAT TGTTTATAAT TTTTTAGAAA AAAAACTTGA AGATAAAATA ACAACAATAA 841 TAGTTTCAAA AGCACATTTT TGTTGTAAAT TTCAAGAAAA ATACAATCAT ATTACTGATT 901 GACTACAAAA ATTTGTAAAT TAATCCAGGC TACATGTTTA AGATGTGTCA AAACTGCATC 961 ATCTACATCT CATTTTAATT TTTCTAAACT ACATACATAG TATCAATAAC AATTGAACAA 1021 TGCTAGTTAA TGTGTTATAC TCATTAGCTT GTAATATATC TTCAACTTGA TGAATCGGAT 1081 TGATCATAAA ATTTCACAAA CTTATGTGCT GATTAATAAA AAATATAATC AGATTTTTCA 1141 TGAATAATTA AGATAAATAA CACATGCAAG TCTCAATTAG AATTTAACAA TCTGACTATA 1201 AAAAGTCGGT TTATCTTGAT TTTCACAAAA ATTTAACATA GTTTCAAGGT GAATGGGCTT 1261 TTATTTTAAT CCAATCAACT ATTTGACACA TACATCTTGC AAATTTATTT ATTTTTTATG 1321 GAAAAAAACA TGATTTATGT TTTTCTTTGA GAAAGTATAT TGTGGATTGT CAAAAAAAAC 1381 CTATGGAAGA TTTATATGTA AGAGTTAAAA ATTTATTTTC AAATATGTCA ACAATCCACA 1441 ATATCGAAGA AAATTTTCAA AAAATCATCA CATATGATGT AAATATAATA TGAATATGTG 1501 CAATCTTATT TCATTTTACT CTGAAAACTT AATTTCTTTT TATAGAGCAT TCTGTACTTA 1561 CTGGTGTCAA ATATACAGAT AAAATGCAGT CCATAACGTA TCACTCTATA AGCAAAATCA 1621 TAAACTTCGT GATCTCTCAC AGTTCAAACG AGAACTATAG AAACCCTTCA ACTAGCAAAA 1681 AACTCACAAC TTTCTATTCC AATATCTTTA ATTGTCTGAC GATTTACAAA TAGAAACGAA 1741 AACCAGACAC ACACACACAC GCAAACTACA AAGTAAAAAC ACAACCACCT GACATTAGAC 1801 AGTGAGATTA TTAAACATCC TAAACTAAAA AACAAACAAA TTAATTCTCT ATGTACAAGT 1861 CCCCATCGTG ATCAATTTTT GCATCCCAAT ATAGTGGCGG TTAGTATAAG AGGACCTTCG 1921 TCTATTCACT CTCCATGTTT GTCAAACTAT AGTACAGTAC ATATATGTAT GGGAACAAAT 1981 TCCGAACACT CTAGTACACT CCTCAACGCA AGTTCTATCT CATATCTCTG CAAGAGCTTA 2041 TTCCAAGAAA CTTTCTTGTG CTTCTATGCA TGCCCAGGTA CCTTAAGTCG CGCGCATAGT 2101 CACTTCACAG CCTTTTCAGT TCTCCCTTTG ATGGCTCAGA GTGACGCAAC TGCACAAAAA 2161 TAAGAAAAAA GTCGAAGCGT CACGAGAGAT GTATCCGGCT AGTTTCCTTG GAGTTCGGAT 2221 TGCTCTCTTT CTCTGTCTCC CCGAACCATA TGGAATTTAT GAACTGCTGT TCACTAGTCA 2281 GTCACTTGTT TCAGAACTTT TGAGTGGTCG ATTTATCCAC CTTCTTGTTT CTTCCTGGGT 2341 CCAGAATCTG ACTTCTTGAG ACAACAAACA GTGCACAGTG GATCGAAGAA TGACCACAGT 2401 TGAGACACGT GGCGCTGGCC TCATTTATTG CACCATACGT TTAGTGAAGA ACTGAATAAT 2461 GGTGGATATA AAGCAGAGTT GCACCCAGCC tCTCGATgtc ttccacagct tgtctgtaag 2521 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 2581 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 2641 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcatcc gccattcagg 2701 ctgcgcaact gttgggaagg gcgatcggcg caggcctctt cgctattacg ccagctggcg 2761 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 2821 cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc ggggatcctc tagagtcgac 2881 ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 2941 tccgctcaca attccacaca acatacgagc cggaagcata tagtgtaaag cctggggtgc 3001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 3061 aaacctgtcg tgccagctgg gaagacgttt tagagctaga aatagcaagt taaaataagg 3121 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttC TAGACCCAGC 3181 TTTCTTGTAC AAAGTTGGCc gctatactga agagcaattc tgacaggata tattggcggg 3241 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag 3301 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa 3361 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 3421 cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 3481 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 3541 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 3601 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 3661 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 3721 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 3781 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 3841 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 3901 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 3961 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 4021 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 4081 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 4141 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 4201 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 4261 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 4321 gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg 4381 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg 4441 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 4501 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 4561 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 4621 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 4681 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 4741 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 4801 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 4861 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 4921 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 4981 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 5041 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 5101 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 5161 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 5221 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 5281 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 5341 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 5401 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 5461 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 5521 acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga 5581 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg 5641 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc 5701 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 5761 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 5821 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 5881 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 5941 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 6001 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 6061 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 6121 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 6181 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 6241 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 6301 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga 6361 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 6421 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 6481 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 6541 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 6601 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 6661 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 6721 aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 6781 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 6841 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 6901 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 6961 aagtgctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 7021 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 7081 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc 7141 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg 7201 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 7261 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 7321 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 7381 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcatcc gcttcctcgc 7441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 8041 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 8101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 8161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 8221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt 8281 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca 8341 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc 8401 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa 8461 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg 8521 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc 8581 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat 8641 tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc 8701 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga 8761 tattcttcta atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca 8821 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag 8881 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga 8941 aacaactctg gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg 9001 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc 9061 ggccttgagc aagacgtttc ccgttgaata tggctcat //