LOCUS       OP_085                  3405 bp    DNA     circular     29-Apr-2025
ACCESSION   OP_085
VERSION     OP_085.1  
FEATURES             Location/Qualifiers
     misc_feature    256..261
                     /label=BsaI recognition site
     misc_feature    complement(872..877)
                     /label=BsaI recognition site
     misc_feature    855..861
                     /label=SapI recognition site
     misc_feature    complement(272..278)
                     /label=SapI recognition site
     oriT            complement(3265..3405)
                     /label=basis of mobility region from pBR322
     rep_origin      complement(2491..3079)
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication 2
     promoter        complement(735..764)
                     /label=lac promoter
     misc_feature    complement(708..728)
                     /label=lac operator
     CDS             complement(369..690)
                     /label=LacZa
     CDS             1393..2403
                     /label=aadA-1 / Sm resistance
     rep_origin      2480..3094
                     /label=rep (pMB1)
ORIGIN
        1 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
       61 gcttacagac aagctgtgac cgtcaccggg agctgcatgt gtcagaggtt ttcaccgtca
      121 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
      181 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
      241 aCTGCAGGCG GCCGCggtct caaatgaatg tgaagagcca cagcttgtct gtaagcggat
      301 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
      361 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
      421 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct catccgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggcgcaggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
      661 ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      721 tcacaattcc acacaacata cgagccggaa gcatatagtg taaagcctgg ggtgcctaat
      781 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      841 tgtcgtgcca gctggctctt caggtagctt tgagaccGCG GCCGCGAATT Catcagtccg
      901 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
      961 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     1021 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc
     1081 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     1141 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     1201 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa gttctgttcc
     1261 agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg
     1321 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     1381 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     1441 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     1501 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     1561 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa
     1621 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1681 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1741 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1801 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1861 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1921 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1981 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     2041 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     2101 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggtgatgag
     2161 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     2221 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     2281 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     2341 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     2401 taatcaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3241 agtgagcgag gaagcggatg agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3301 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3361 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcg
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