LOCUS       OP_091                  2219 bp    DNA     circular     29-Apr-2025
ACCESSION   OP_091
VERSION     OP_091.1  
FEATURES             Location/Qualifiers
     misc_feature    269..274
                     /label=BsaI recognition site
     misc_feature    complement(425..430)
                     /label=BsaI recognition site
     misc_feature    261..267
                     /label=SapI recognition site
     misc_feature    complement(432..438)
                     /label=SapI recognition site
     rep_origin      1631..2219
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
     CDS             674..1333
                     /label=cat / CmR
     terminator      1354..1448
                     /label=lambda t0 terminator
     terminator      280..418
                     /label=BBa_B0015
     terminator      291..370
                     /label=BBa_B0010
     terminator      379..419
                     /label=BBa_B0012
     misc_feature    406..419
                     /label=T7 TE
ORIGIN
        1 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
       61 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
      121 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCCTGC
      181 ATAACGCGAA GTAATCTTTT CGGTTTTAAA GAAAAAGGGC AGGGTGGTGA CACCTTGCCC
      241 TTTTTTGCCG GACTGCAGGG GCTCTTCAGG TCTCTGCTTt aatactagag ccaggcatca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      361 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttataC
      421 GCTTGAGacc TGAAGAGCGA ATTCCAGAAA TCATCCTTAG CGAAAGCTAA GGATTTTTTT
      481 TATCTGAAAT TCTGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG
      541 GTTTCTTAGA CGTCAGGTGG CACTCGAGTT GATCGGGCAC GTAAGAGGTT CCAACTTTCA
      601 CCATAATGAA ATAAGATCAC TACCGGGCGT ATTTTTTGAG TTATCGAGAT TTTCAGGAGC
      661 TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG
      721 GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC
      781 CGTTCAGCTG GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA
      841 TCCGGCCTTT ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TTCGTATGGC
      901 AATGAAAGAC GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA
      961 TGAGCAAACT GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT
     1021 TCTACACATA TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCTGGCCT ATTTCCCTAA
     1081 AGGGTTTATT GAGAATATGT TTTTCGTCTC AGCCAATCCC TGGGTGAGTT TCACCAGTTT
     1141 TGATTTAAAC GTGGCCAATA TGGACAACTT CTTCGCCCCC GTTTTCACCA TGGGCAAATA
     1201 TTATACGCAA GGCGACAAGG TGCTGATGCC GCTGGCGATT CAGGTTCATC ATGCCGTTTG
     1261 TGATGGCTTC CATGTCGGCA GAATGCTTAA TGAATTACAA CAGTACTGCG ATGAGTGGCA
     1321 GGGCGGGGCG TAATTTGATA TCGAGCTCGC TTGGACTCCT GTTGATAGAT CCAGTAATGA
     1381 CCTCAGAACT CCATCTGGAT TTGTTCAGAA CGCTCGGTTG CCGCCGGGCG TTTTTTATTG
     1441 GTGAGAATCC AAGCCTCGAG CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
     1501 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
     1561 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     1621 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     1681 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     1741 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG
     1801 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     1861 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     1921 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     1981 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     2041 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     2101 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     2161 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTGTGGAAA
//