LOCUS OP_095 8158 bp DNA circular 29-Apr-2025 ACCESSION OP_095 VERSION OP_095.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(2269..2275) /label=SapI recognition site oriT 6341..6481 /label=basis of mobility region from pBR322 rep_origin 6667..7255 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 3606..4235 /label=pVS1 StaA stability protein CDS 4664..5737 /label=pVS1 RepA replication protein rep_origin 5803..5997 /label=pVS1 oriV origin of replication misc_feature 3367..4367 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 2281..2305 /label=A.tumefaciens plasmid right boundary CDS complement(7343..8158) /label=nptI / KanR misc_feature 1130..1136 /label=TATA box misc_feature 2167..2173 /label=TATA box rep_origin complement(6652..7266) /label=rep (pMB1) misc_feature 484..2259 /label=trnG–trnfM HR right ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcacagag 481 gagtaagtaa aaatatttTT TTTTGttttt tggcggagac aggatttgaa cctatgacct 541 caaggttatg agccttgcga gctaccagac tgctctactc cgcgttataa ttaacgaaaa 601 aagtatactc tataaaaatA gaattaaGca agatatAttt tAaaGaaccc cgcctcttat 661 aagaggcggg aaagaaacTc aaaatgttta ccaactagat ttTgttactc caggcaataa 721 acatgcatga gccatttcac gaagtaaatg tctagataaa ccaaaatcgc gGtaatttgc 781 tttaggtctt ccagtcaaaa aacaacgacg atgaagacga gtcggggcac tattacgtgg 841 taaagattgt aatttttttt gaaattccca tttCtcatct aatgatgaAg tttccgtaat 901 tttCtttttt aaagaattac gtaaaGtttt gtattttttt tctaaatttt gtcttttttt 961 ttctctttga ataagactct tttttgccat aatAatttaa ttagtaagat attctttttc 1021 Gataaatttt tattaaaTTa tattttTagt aaaattggaa atttaCtaca aatataattt 1081 aaccaaattt accagaCgta gaagcaatta aaaaagcagc ataagtaaat atataaccta 1141 cagaaaaatg agctaatcca actaatcttg cttgaacaat agaaagagct actggtttat 1201 ctttccagcg aactaaattc gctaaaggag tacgttcgtg agcccaagct aaagtttcaa 1261 taagttcttg ccaatatcca cgccaTgata tcagaaacat aaatccagta gcccaaacta 1321 aatgaccaaa taaaaacatc catgcccaaa cagacaAact Gttcatacca aaaggattat 1381 atccattaat caattgtgaa gaatttaacc ataaataatc tcttaaccag cccattaaat 1441 atgtagaaga ttcattaaat tgtgctgcat ttccttgcca taatgtaata tgtttccaat 1501 gccaataaaa agtaacccat ccaatagtat ttaacatcca aaaaactgct aaataaaaag 1561 catcccaagc agaaatatca caagtaccac ctcgtccagg accatcacaa ggaaaactat 1621 aaccaaattc ttttttatct ggcattaatt tagatcctcg tgcatctaaa gcacctttca 1681 ctaaaattaa tgtagtagta tgtaaaccta aagcaatagc gtgatgtact aaaaagtctc 1741 caggaccaat tgttaagaaa agtgaattac tattattatt Gatagcatct aaccaCccag 1801 gtaaccatat Actttgacca gcattaaatg ctggattatt tgttgatgat aaaagtacat 1861 caaaaccata taaagcttta ccatgagcag attgtatcca ttgagcaaaa ataggttcaa 1921 ttaaaatttg tttttcagga gtaccaaaag caagcataac atcattatga acgtaaagac 1981 ctaaagtatg aaaCcctaga aataaactag cccaacttaa atgggatatt atagcttctt 2041 tatgttctaa cattctagct aatacattat ctttattttg ttccggatta taatctctaa 2101 taaagaaaat agctccGtga gcaaaGgcac ctgtcataat aaaaccagca atatattgat 2161 gatgagtata taatgcCgct tgcgttgtaa aatcttgtgc aagaaacgca taCggaggta 2221 aagaatacat atgttgagct actaaggagg taataactcc gctaggttga agagcaattc 2281 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 2341 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 2401 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg 2461 cttctgacgt tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt cctaagttac 2521 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat 2581 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc 2641 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc 2701 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg 2761 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt 2821 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat 2881 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc 2941 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat 3001 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc 3061 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg 3121 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc 3181 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga 3241 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca 3301 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag 3361 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg 3421 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg 3481 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc 3541 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg 3601 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg 3661 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg 3721 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg 3781 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg 3841 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg 3901 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg 3961 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg 4021 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt 4081 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg 4141 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg 4201 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag 4261 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac 4321 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga 4381 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct 4441 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa 4501 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg 4561 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc 4621 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc 4681 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc 4741 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca 4801 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc 4861 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga 4921 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg 4981 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc 5041 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc 5101 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt 5161 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca 5221 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg 5281 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag 5341 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc 5401 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc 5461 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc 5521 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc 5581 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc 5641 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg 5701 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca 5761 aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata gcacgtacat 5821 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta 5881 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc 5941 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact 6001 gtctggccag cgcacagccg aagtgctgca aaaagcgcct acccttcggt cgctgcgctc 6061 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg 6121 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg 6181 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 6241 acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 6301 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 6361 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 6421 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 6481 gcgctcatcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 6541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 7021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 7081 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 7141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7321 tttggtcatg cattctaggt gattagaaaa actcatcgag catcaaatga aactgcaatt 7381 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag 7441 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga 7501 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg 7561 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt 7621 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca 7681 aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag 7741 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa 7801 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg 7861 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag 7921 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc 7981 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcggtaga 8041 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat 8101 ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata tggctcat //