LOCUS OP_096 7906 bp DNA circular 17-Jun-2025 ACCESSION OP_096 VERSION OP_096.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(2017..2023) /label=SapI recognition site oriT 6089..6229 /label=basis of mobility region from pBR322 rep_origin 6415..7003 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 3354..3983 /label=pVS1 StaA stability protein CDS 4412..5485 /label=pVS1 RepA replication protein rep_origin 5551..5745 /label=pVS1 oriV origin of replication misc_feature 3115..4115 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 2029..2053 /label=A.tumefaciens plasmid right boundary CDS complement(7091..7906) /label=nptI / KanR rep_origin complement(6400..7014) /label=rep (pMB1) misc_feature 484..2007 /label=rbcL-trnR HR left ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcaatgag 481 gagCAGCATT TAGAATGACT CCTCAACCTG GAGTTCCAGC AGAAGAAGCA GGAGCAGCAG 541 TTGCTGCTGA GTCTTCAACT GGTACATGGA CTACAGTTTG GACTGATGGT CTTACTAACC 601 TTGATCGTTA TAAAGGTCGA TGCTATGATA TTGACCCTGT TCCTGGAGAA GATAATCAAT 661 ATATTGCTTA TGTAGCTTAT CCTTTAGATT TATTTGAAGA AGGTTCTGTT ACAAATATGT 721 TTACTTCAAT TGTAGGTAAT GTATTTGGGT TTAAAGCTTT AAGAGCGTTA CGTCTTGAAG 781 ATTTAAGAAT TCCTCCAGCT TACACAAAAA CTTTCCAAGG TCCTCCTCAT GGTATTCAAG 841 TTGAGAGAGA TAAATTAAAC AAATATGGTC GTCCTTTATT AGGATGTACT ATTAAACCAA 901 AATTAGGTTT ATCTGCTAAA AACTATGGTA GAGCTGTATA TGAATGTCTT CGTGGTGGAC 961 TTGATTTTAC TAAAGATGAT GAAAACGTAA ATTCTCAACC ATTTATGCGT TGGAGAGATC 1021 GTTTCTTATT TGTAGCAGAA GCTATTTTTA AATCTCAAGC AGAAACTGGA GAAATCAAAG 1081 GACATTATTT AAATGCTACT GCAGGTACAT GTGAAGAAAT GCTAAAAAGA GCAGCATGTG 1141 CTAGAGAGTT AGGTGTACCA ATTGTTATGC ACGACTACTT AACTGGTGGT TTCACTGCAA 1201 ATACTACTCT AGCTTTTTAT TGCCGTGACA ATGGTTTACT TCTTCATATT CACCGTGCAA 1261 TGCATGCAGT TATTGATAGA CAAAAAAATC ATGGTATCCA CTTCCGTGTA TTAGCAAAAG 1321 CTTTACGTAT GTCTGGTGGA GATCATATTC ACGCTGGTAC TGTTGTAGGT AAACTTGAAG 1381 GAGAtCGTAA CGTAACTTTA GGTTTCGTAG ATTTACTTCG TGATGACTAT ATTGAAAAAG 1441 ATAGAAGTCG TGGTATTTAT TTCACACAAG ATTGGGTTTC TTTACCTGGT GTTTTCCCTG 1501 TAGCATCTGG TGGGATCCAT GTTTGGCATA TGCCTGCTTT AACTGAAATT TTTGGCGATG 1561 ACTCTGTTTT ACAATTTGGT GGTGGAACTT TAGGTCATCC TTGGGGTAAC GCACCTGGTG 1621 CAGTTGCTAA CCGAGTTTCG TTAGAAGCTT GTGTACAAGC ACGTAATGAA GGTCGTGACC 1681 TTGCTCGTGA AGGAAATGAA ATTATTCGTG AAGCTTGCAA ATGGAGTCCT GAGTTATCTG 1741 CTGCTTGTGA AGTTTGGAAA GAAATTAAAT TTGAATTTGA TATTATTGAT ACTTTGTAAA 1801 ATGAAAGTAG ATATACTTTG TAAAATAAAG TAGATATTTT ATCTTAAAAG TTTTGTAATT 1861 TTCTTTTTTT TATCTGATAT TTCAGATAAA AAAAAGAAAA TTACAAAACT TTTAAATGGG 1921 TTTGTAGCTC AGTGGATTAG AGCTCATGGT TCCGGATCAT GAAGTCAAGG GTTCGAATCC 1981 CTTCTAACCC TTTTTTTATT TTGAAAACGC Tagcatgaag agcaattctg acaggatata 2041 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc 2101 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg 2161 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc 2221 agtgcagccg tcatctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc 2281 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac 2341 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg 2401 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc 2461 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga 2521 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga 2581 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg 2641 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat 2701 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac 2761 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct 2821 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa 2881 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag 2941 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac 3001 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg 3061 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc 3121 gcggccgggt acgtgttcga gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc 3181 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa 3241 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg 3301 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg 3361 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag 3421 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca 3481 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc 3541 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg 3601 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga 3661 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta 3721 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga 3781 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca 3841 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa 3901 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg 3961 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa 4021 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct 4081 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa 4141 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat 4201 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta 4261 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt 4321 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg 4381 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt 4441 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc 4501 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga 4561 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc 4621 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg 4681 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc 4741 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc 4801 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga 4861 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca 4921 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct 4981 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc 5041 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat 5101 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta 5161 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt 5221 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc 5281 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa 5341 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt 5401 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga 5461 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc 5521 aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc acgtacattg ggaacccaaa 5581 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg 5641 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc 5701 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg 5761 cacagccgaa gtgctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc 5821 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg 5881 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca 5941 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 6001 cggtgacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 6061 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 6121 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 6181 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcatccgc 6241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 6961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca 7081 ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag 7141 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga 7201 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat 7261 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat 7321 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt 7381 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca 7441 ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga caattacaaa 7501 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg 7561 aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca gtggtgagta 7621 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg 7681 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat 7741 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt gtcgcacctg 7801 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat 7861 ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcat //