LOCUS OP_098 7738 bp DNA circular 29-Apr-2025 ACCESSION OP_098 VERSION OP_098.1 FEATURES Location/Qualifiers misc_feature 468..474 /label=SapI recognition site misc_feature complement(1849..1855) /label=SapI recognition site oriT 5921..6061 /label=basis of mobility region from pBR322 rep_origin 6247..6835 /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of replication 2 CDS 3186..3815 /label=pVS1 StaA stability protein CDS 4244..5317 /label=pVS1 RepA replication protein rep_origin 5383..5577 /label=pVS1 oriV origin of replication misc_feature 2947..3947 /label=STA region from pVS1 plasmid misc_feature 433..458 /label=A.tumefaciens plasmid left boundary misc_feature 1861..1885 /label=A.tumefaciens plasmid right boundary CDS complement(6923..7738) /label=nptI / KanR misc_feature complement(1693..1710) /label=rbcL 5'UTR promoter complement(1693..1839) /label=PROM5_Ntprrn CDS complement(897..1691) /label=aadA gene / SptR terminator complement(484..892) /label=Nt psbA terminator rep_origin complement(6232..6846) /label=rep (pMB1) ORIGIN 1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta 61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga 121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga 181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta 241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag 301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg 361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg 421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcagcaag 481 gagCGATAAG CTTCGAATAT AGaTCTTCTT TCTTATTTCA ATGATATTAT TATTTCAAAG 541 ATAAGAGATA TTCAAAGATA AGAGATAAGA AGAAGTCAAA ATTTGATTTT TTTTTGGAAA 601 AAAAAAATCA AAAAGATATA GTAACATTAG CAAGAAGAGA AACAAGTTCT ATTTCTACAA 661 TTTTAAACAA ATACAAAATC AAAATAGAAT ACTCAATCAT GAATAAATGC AAGAAAATAA 721 CCTCTCCTTC TTTTTCTATA ATGTAAACAA AAAAGTCTAT GTAAGTAAAA TACTAGTAAA 781 TAAATAAAAA GAAAAAAAGA AAGGAGCAAT AGCACCCTCT TGATAGAACA AGAAAATGAT 841 TATTGCTCCT TTCTTTTCAA AACCTCCTAT AGACTAGGCC AGGATCCTGC AGAAGCTTAT 901 TTGCCAACTA CCTTAGTGAT CTCGCCTTTC ACGTAGTGGA CAAATTCTTC CAACTGATCT 961 GCGCGCGAGG CCAAGCGATC TTCTTCTTGT CCAAGATAAG CCTGTCTAGC TTCAAGTATG 1021 ACGGGCTGAT ACTGGGCCGG CAGGCGCTCC ATTGCCCAGT CGGCAGCGAC ATCCTTCGGC 1081 GCGATTTTGC CGGTTACTGC GCTGTACCAA ATGCGGGACA ACGTAAGCAC TACATTTCGC 1141 TCATCGCCAG CCCAGTCGGG CGGCGAGTTC CATAGCGTTA AGGTTTCATT TAGCGCCTCA 1201 AATAGATCCT GTTCAGGAAC CGGATCAAAG AGTTCCTCCG CCGCTGGACC TACCAAGGCA 1261 ACGCTATGTT CTCTTGCTTT TGTCAGCAAG ATAGCCAGAT CAATGTCGAT CGTGGCTGGC 1321 TCGAAGATAC CTGCAAGAAT GTCATTGCGC TGCCATTCTC CAAATTGCAG TTCGCGCTTA 1381 GCTGGATAAC GCCACGGAAT GATGTCGTCG TGCACAACAA TGGTGACTTC TACAGCGCGG 1441 AGAATCTCGC TCTCTCCAGG GGAAGCCGAA GTTTCCAAAA GGTCGTTGAT CAAAGCTCGC 1501 CGCGTTGTTT CATCAAGCCT TACGGTCACC GTAACCAGCA AATCAATATC ACTGTGTGGC 1561 TTCAGGCCGC CATCCACTGC GGAGCCGTAC AAATGTACGG CCAGCAACGT CGGTTCGAGA 1621 TGGCGCTCGA TGACGCCAAC TACCTCTGAT AGTTGAGTTG ATACTTCGGC GATAACCGCT 1681 TCACGAGCCA TTGGATCCCT CCCTACAACT GTATCCAAGC GCTTCAAATT CGCCCGGAGT 1741 TCGCTCCCAG AAATATAGCC ATCCCTGCCC CCTCACGTCA ATCCCACGAG CCTCTTATCC 1801 ATTCTCATTG AACGACGGCG GGGGAGCAAA TCCAACTAGc gctatactga agagcaattc 1861 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 1921 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 1981 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg 2041 cttctgacgt tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt cctaagttac 2101 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat 2161 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc 2221 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc 2281 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg 2341 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt 2401 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat 2461 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc 2521 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat 2581 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc 2641 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg 2701 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc 2761 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga 2821 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca 2881 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag 2941 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg 3001 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg 3061 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc 3121 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg 3181 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg 3241 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg 3301 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg 3361 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg 3421 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg 3481 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg 3541 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg 3601 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt 3661 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg 3721 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg 3781 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag 3841 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac 3901 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga 3961 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct 4021 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa 4081 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg 4141 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc 4201 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc 4261 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc 4321 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca 4381 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc 4441 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga 4501 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg 4561 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc 4621 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc 4681 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt 4741 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca 4801 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg 4861 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag 4921 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc 4981 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc 5041 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc 5101 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc 5161 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc 5221 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg 5281 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca 5341 aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata gcacgtacat 5401 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta 5461 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc 5521 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact 5581 gtctggccag cgcacagccg aagtgctgca aaaagcgcct acccttcggt cgctgcgctc 5641 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg 5701 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg 5761 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 5821 acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 5881 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 5941 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 6001 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 6061 gcgctcatcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 6121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6661 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 6721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 6841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 6901 tttggtcatg cattctaggt gattagaaaa actcatcgag catcaaatga aactgcaatt 6961 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag 7021 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga 7081 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg 7141 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt 7201 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca 7261 aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag 7321 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa 7381 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg 7441 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag 7501 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc 7561 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcggtaga 7621 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat 7681 ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata tggctcat //