LOCUS       ODC_0089                2789 bp    DNA     circular     29-Apr-2025
ACCESSION   ODC_0089
VERSION     ODC_0089.1  
FEATURES             Location/Qualifiers
     misc_feature    2783..2788
                     /label=BsaI_site
     misc_feature    complement(676..681)
                     /label=BsaI_site
     rep_origin      complement(933..1520)
                     /label=ori
     terminator      2712..2743
                     /label=tonB_terminator
     CDS             complement(1712..2571)
                     /label=AmpR
     promoter        complement(2573..2676)
                     /label=AmpR_promoter
     terminator      800..847
                     /label=T7_terminator
     promoter        2591..2596
                     /label=minus_10
     promoter        complement(2335..2339)
                     /label=minus_10
     promoter        complement(769..773)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1524..1713
                     /label=spacer
     misc_feature    1528..1547
                     /label=anneal_sequence
     CDS             884..934
                     /label=spacer
     misc_feature    762..2762
                     /label=pBuild__pOpen_v3 backbone
     promoter        complement(351..355)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    complement(561..568)
                     /label=3xSTOP
     CDS             5..670
                     /label=gfasPurple_gfasCP__Biobrick_K1033918
     misc_feature    1..4
                     /label=AATG
     misc_feature    671..674
                     /label=GCTT
ORIGIN
        1 AATGTCGGTG ATTGCTAAAC AGATGACCTA CAAAGTCTAT ATGTCGGGTA CGGTGAACGG
       61 CCATTATTTT GAAGTTGAAG GTGACGGTAA AGGCAAGCCG TATGAAGGCG AACAGACCGT
      121 TAAACTGACC GTCACGAAGG GCGGTCCGCT GCCGTTTGCA TGGGATATTC TGAGTCCGCA
      181 GTCCCAATAT GGCAGCATCC CGTTCACGAA ATATCCGGAA GATATCCCGG ACTACGTGAA
      241 GCAGTCTTTT CCGGAAGGTT ACACCTGGGA ACGTATCATG AACTTCGAAG ATGGCGCCGT
      301 CTGCACCGTG AGTAACGACA GCTCTATTCA AGGTAATTGT TTCATCTACC ATGTCAAGTT
      361 CTCAGGTCTG AACTTCCCGC CGAATGGCCC GGTGATGCAG AAAAAGACCC AAGGCTGGGA
      421 ACCGAATACG GAACGTCTGT TTGCACGCGA TGGTATGCTG ATTGGCAACA ATTTCATGGC
      481 TCTGAAACTG GAAGGCGGTG GCCACTATCT GTGCGAATTT AAAAGCACCT ACAAGGCGAA
      541 AAAGCCGGTT AAAATGCCGG GCTATCATTA CGTGGATCGT AAACTGGACG TTACCAACCA
      601 CAATAAGGAC TATACGTCCG TCGAACAGTG TGAAATTTCA ATCGCGCGCA AATCGGTGGT
      661 TGCCTGAAGA GCTTTGAGAC CCAGCCTCTG TGTTGTCTGA TGGCTCGGCC TGATTGAGCT
      721 CTTAGGGATA ACAGGGTAAT AATTTATGGC CAGCGGATGC GCGCTGAGGT GTCAATCGTC
      781 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
      841 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
      901 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
      961 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1021 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1081 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1141 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1201 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1261 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1321 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1381 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1441 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1501 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     1561 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     1621 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     1681 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     1741 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     1801 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     1861 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     1921 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     1981 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2041 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2101 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2161 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2221 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2281 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     2341 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     2401 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     2461 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     2521 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     2581 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     2641 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     2701 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG
     2761 AGAAATAAGA CGACGACCCT CGGGTCTCT
//