LOCUS       ODC_0094                2819 bp    DNA     circular     29-Apr-2025
ACCESSION   ODC_0094
VERSION     ODC_0094.1  
FEATURES             Location/Qualifiers
     misc_feature    2813..2818
                     /label=BsaI_site
     misc_feature    complement(706..711)
                     /label=BsaI_site
     rep_origin      complement(963..1550)
                     /label=ori
     terminator      2742..2773
                     /label=tonB_terminator
     CDS             complement(1742..2601)
                     /label=AmpR
     promoter        complement(2603..2706)
                     /label=AmpR_promoter
     terminator      830..877
                     /label=T7_terminator
     promoter        2621..2626
                     /label=minus_10
     promoter        complement(2365..2369)
                     /label=minus_10
     promoter        complement(799..803)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1554..1743
                     /label=spacer
     misc_feature    1558..1577
                     /label=anneal_sequence
     CDS             914..964
                     /label=spacer
     misc_feature    792..2792
                     /label=pBuild__pOpen_v3 backbone
     CDS             5..700
                     /label=aacuGFP1
     misc_feature    1..4
                     /label=AATG
     misc_feature    701..704
                     /label=GCTT
ORIGIN
        1 AATGTCTCTG AGCAAACATG GTATTACCCA GGAAATGCCG ACCAAGTACC ACATGAAAGG
       61 CTCCGTGAAC GGCCACGAGT TCGAAATTGA AGGTGTAGGC ACCGGTCATC CGTACGAAGG
      121 AACCCATATG GCTGAGCTGG TGATCATCAA ACCGGCCGGT AAACCGCTTC CGTTCTCCTT
      181 CGATATCCTG TCTACTGTGA TTCAGTACGG CAACCGTTGC TTCACCAAGT ACCCGGCGGA
      241 TCTGCCGGAC TATTTCAAAC AGGCGTATCC GGGCGGCATG TCTTACGAAC GTAGCTTCGT
      301 GTACCAGGAT GGCGGTATTG CAACCGCGTC TTGGAACGTG GGCCTGGAAG GCAATTGTTT
      361 CATCCACAAA TCTACTTATC TGGGTGTTAA CTTTCCGGCT GACGGTCCAG TAATGACCAA
      421 AAAAACCATC GGTTGGGACA AGGCTTTCGA AAAAATGACT GGTTTCAACG AAGTTCTGCG
      481 TGGCGATGTT ACCGAGTTCC TGATGCTGGA GGGCGGTGGC TACCACTCTT GCCAGTTCCA
      541 CTCCACCTAT AAACCGGAAA AGCCGGTGGA ATTGCCGCCG AACCACGTAA TTGAACATCA
      601 TATTGTTCGT ACCGATCTTG GAAAAACCGC CAAAGGTTTC ATGGTGAAGC TGGTACAGCA
      661 CGCGGCGGCA CACGTGAACC CGCTGAAAGT ACAGTGAAGA GCTTTGAGAC CCGCTAAGAT
      721 CCGTAGGTCT GATCGGAGAT CCCAAGATCG AGTAGGGATA ACAGGGTAAT GTGCCTGGCG
      781 GATAGCGGGA CCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
      841 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
      901 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
      961 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1021 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1081 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1141 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1201 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1261 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1321 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1381 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1441 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1501 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     1561 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     1621 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     1681 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     1741 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     1801 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     1861 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     1921 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     1981 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2041 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2101 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2161 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2221 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2281 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2341 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     2401 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     2461 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     2521 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     2581 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     2641 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     2701 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     2761 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AGAAATAAGA CGACGACCCT CGGGTCTCT
//