LOCUS       ODC_0095                2819 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0095
VERSION     ODC_0095.1  
FEATURES             Location/Qualifiers
     misc_feature    2813..2818
                     /label=BsaI_site
     misc_feature    complement(706..711)
                     /label=BsaI_site
     rep_origin      complement(963..1550)
                     /label=ori
     terminator      2742..2773
                     /label=tonB_terminator
     CDS             complement(1742..2601)
                     /label=AmpR
     promoter        complement(2603..2706)
                     /label=AmpR_promoter
     terminator      830..877
                     /label=T7_terminator
     promoter        2621..2626
                     /label=minus_10
     promoter        complement(2365..2369)
                     /label=minus_10
     promoter        complement(799..803)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1554..1743
                     /label=spacer
     misc_feature    1558..1577
                     /label=anneal_sequence
     CDS             914..964
                     /label=spacer
     misc_feature    792..2792
                     /label=pBuild__pOpen_v3 backbone
     CDS             5..700
                     /label=aacuGFP2
     misc_feature    1..4
                     /label=AATG
     misc_feature    701..704
                     /label=GCTT
ORIGIN
        1 AATGTCTTAC TCTAAACAAG GTATCGTTCA GGAGATGAAA ACTAAATACC GCATGGAAGG
       61 TTCCGTCAAC GGTCACGAGT TCACCATTGA AGGCGTGGGT ACCGGTTACC CGTACGAAGG
      121 TAAACAGATG TCAGAGCTGG TTATCATTAA ACCGAAAGGC AAGCCGCTGC CGTTTTCATT
      181 CGATATCCTG AGCAGCGTGT TCCAGTACGG TAACCGTTGC TTTACTAAAT ACCCGGCTGA
      241 TATGCCCGAC TATTTCAAAC AGGCATTCCC AGATGGCATG AGTTACGAAC GTTCTTTCCT
      301 GTTTGAGGAC GGTGCAGTGG CAACCGCCAG CTGGAACATC CGCCTCGAAG GTAACTGTTT
      361 CATCCACAAT TCCATTTTCC ACGGCGTCAA CTTCCCGGCG GATGGCCCGG TTATGAAAAA
      421 GAAAACTATT GGTTGGGATA AGTCTTTTGA AAAAATGACC GTCAGCAAAG AGGTTCTGCG
      481 TGGTGATGTT ACTATGTTCC TGATGCTGGA AGGTGGCGGT TACCACCGTT GCCAGTTCCA
      541 CAGCACGTAC AAAACCGAGA AACCGGTGGA ACTGCCGCCG AACCACGTTG TAGAACACCA
      601 AATCGTTCGC ACGGATCTGG GTCAAAGCGC TAAAGGCTTT ACCGTTAAAC TGGAAGCGCA
      661 CGCCGCCGCT CATGTTAACC CGCTGAAAGT CCAGTGAAGA GCTTTGAGAC CGCCATCCTC
      721 TTATCTCGTG GAGACGCTTC CCATGCTGTG GGTAGGGATA ACAGGGTAAT GGAGGTCCGC
      781 GTTTAAGCTT ACGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
      841 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
      901 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
      961 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     1021 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     1081 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     1141 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     1201 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     1261 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     1321 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     1381 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     1441 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     1501 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     1561 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     1621 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     1681 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     1741 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     1801 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     1861 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     1921 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     1981 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     2041 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     2101 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     2161 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     2221 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     2281 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     2341 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     2401 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     2461 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     2521 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     2581 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     2641 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     2701 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     2761 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AGAAATAAGA CGACGACCCT CGGGTCTCT
//