LOCUS       ODC_0096                2804 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0096
VERSION     ODC_0096.1  
FEATURES             Location/Qualifiers
     misc_feature    2798..2803
                     /label=BsaI_site
     misc_feature    complement(691..696)
                     /label=BsaI_site
     rep_origin      complement(948..1535)
                     /label=ori
     terminator      2727..2758
                     /label=tonB_terminator
     CDS             complement(1727..2586)
                     /label=AmpR
     promoter        complement(2588..2691)
                     /label=AmpR_promoter
     terminator      815..862
                     /label=T7_terminator
     promoter        2606..2611
                     /label=minus_10
     promoter        complement(2350..2354)
                     /label=minus_10
     promoter        complement(784..788)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1539..1728
                     /label=spacer
     misc_feature    1543..1562
                     /label=anneal_sequence
     CDS             899..949
                     /label=spacer
     misc_feature    777..2777
                     /label=pBuild__pOpen_v3 backbone
     promoter        complement(474..478)
                     /label=minus_35
     CDS             5..685
                     /label=afraGFP
     misc_feature    1..4
                     /label=AATG
     misc_feature    686..689
                     /label=GCTT
ORIGIN
        1 AATGAGCGTT ATTGTGAAAG AAATGATGAC CAAACTGCAT ATGGAAGGCA CCGTTAACGG
       61 TCACGCGTTC ACAATCGAAG GTAAGGGTAA AGGCGATCCG TACAACGGTG TTCAGTCTAT
      121 GAACCTGGAC GTAAAAGGCG GTGCTCCTCT CCCGTTCAGC TTCGACCTGC TGACTCCGGC
      181 ATTCATGTAC GGCAACCGTG TGTTCACTAA ATACCCGGAG GACATCCCTG ACTTCTTTAA
      241 GCAGGTATTC CCGGAAGGCT ATCACTGGGA ACGTTCCATT ACTTTTGAGG ACCAGGCGGT
      301 GTGCACTGCA ACCTCTCACA TCCGCCTGGA CCAGAAAGAA ATGTGCTTCA TTTATGACGT
      361 TCGTTTTCAC GGCGTTAACT TCCCTGCAAA CGGCCCGATC ATGCAGAAAA AAATTCTGGG
      421 TTGGGAGCCG TCCACCGAAA AAATGTACGC TCGTGACGGT GTACTGAAAG GTGATGTAAA
      481 CATGACCCTG CGCGTTGAGG GTGGCGGTCA CTACCGTGCA GATTTCCGTA CCACTTATAA
      541 AGCTAAAAAG CCGGTAAACC TGCCGGGCTA CCACTTTATT GATCACCGTA TCGAAATCAC
      601 GAAGCACTCT AAAGATTACA CCAACGTAGC GCTGTACGAG GCAGCTGTTG CTCGTCATTC
      661 ACCGCTGCCG AAAGTGGCCT GAAGAGCTTT GAGACCGTAT GTCCCCTAAG CTCTGGTCCA
      721 GACCTACCCA GGGTCCTTAG GGATAACAGG GTAATAAAAA ATAATGCTCC GCACGCCGCT
      781 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      841 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
      901 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
      961 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1021 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1081 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1141 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1201 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1261 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1321 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1381 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1441 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1501 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1561 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1621 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1681 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1741 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     1801 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     1861 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     1921 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     1981 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2041 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2101 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2161 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2221 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2281 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2341 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2401 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2461 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2521 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2581 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2641 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2701 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     2761 TTCAGGTGGA GTGGGAGAAA TAAGACGACG ACCCTCGGGT CTCT
//