LOCUS       ODC_0098                2813 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0098
VERSION     ODC_0098.1  
FEATURES             Location/Qualifiers
     misc_feature    2807..2812
                     /label=BsaI_site
     misc_feature    complement(700..705)
                     /label=BsaI_site
     rep_origin      complement(957..1544)
                     /label=ori
     terminator      2736..2767
                     /label=tonB_terminator
     CDS             complement(1736..2595)
                     /label=AmpR
     promoter        complement(2597..2700)
                     /label=AmpR_promoter
     terminator      824..871
                     /label=T7_terminator
     promoter        2615..2620
                     /label=minus_10
     promoter        complement(2359..2363)
                     /label=minus_10
     promoter        complement(793..797)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1548..1737
                     /label=spacer
     misc_feature    1552..1571
                     /label=anneal_sequence
     CDS             908..958
                     /label=spacer
     misc_feature    786..2786
                     /label=pBuild__pOpen_v3 backbone
     promoter        163..168
                     /label=minus_35_pLlac_y_pLtet
     CDS             5..694
                     /label=eechGFP1
     misc_feature    1..4
                     /label=AATG
     misc_feature    695..698
                     /label=GCTT
ORIGIN
        1 AATGAGCGTT ATCAACCCGG ACATGAAAAT CAAACTGTAT ATGGAGGGCG CGGTTAACGG
       61 CCACAAATTT GAGATCAAAG GCGAAGGCAA CGGTAAGCCA TTCGAAGGCA AACAGACTAT
      121 GGATCTGGCT GTTGTCGACG GCGGTCCACT GCCTTTCGCT TTTGACATCC TGACCACTTC
      181 TTTTAACTAT GGTAACCGTG TTTTCACCAA ATACCCGGAC ACCATCGTTG ACTATTTCAA
      241 GCCGTCATTC CCTGAAGGCT ACTCCTGGGA ACGCAGCATG ACGTATGAGG ACGGTGGTAT
      301 TTGCATCGCA ACTAACGACA TTACCCTCCT GAAAGACACT GATGACAGCA ACTACTTCTA
      361 CTACAAAATC CGTTTCGACG GTGTGAACTT CGCTGCAAAC GGCCCGGTTA TGCAGAAGAA
      421 AACCGCTAAA TGGGAACCGT CAACCGAAAA AATGTACGTT CGTGACGGTG TGCTGAAGGG
      481 CGAAGTTAAC ATGGCGCTGC TCCTGGAAGG TGGTGGTCAT TACCGCTGCG ACTTCAAGAC
      541 TACCTACAAA GCAAAGAAAG TAGTGCGCCT GCCGAGCTAC CACTTCGTCG ACCACCGCAT
      601 CGAAATTCTG TCTCACTCCA AAGACTACAA CCAGGTTCGT CTGCACGAAC ATGCCGAAGC
      661 TCACAGCGGG CTGCCGCGCC AAGCAAAGTG AAGAGCTTTG AGACCCTGTA TCATGGTTCC
      721 CTCGGAAAGT GATACGAACT TCGTTTTAGG GATAACAGGG TAATTTCCAA GCTTGGCGTA
      781 GACTGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
      841 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
      901 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
      961 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1021 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1081 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1141 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1201 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1261 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1321 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1381 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1441 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1501 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     1561 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     1621 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     1681 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     1741 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     1801 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     1861 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     1921 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     1981 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2041 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2101 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2161 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2221 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     2281 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     2341 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     2401 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     2461 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     2521 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     2581 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     2641 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     2701 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     2761 TTGACTTGGT TCAGGTGGAG TGGGAGAAAT AAGACGACGA CCCTCGGGTC TCT
//