LOCUS       ODC_0099                2798 bp    DNA     circular     29-Apr-2025
ACCESSION   ODC_0099
VERSION     ODC_0099.1  
FEATURES             Location/Qualifiers
     misc_feature    2792..2797
                     /label=BsaI_site
     misc_feature    complement(685..690)
                     /label=BsaI_site
     rep_origin      complement(942..1529)
                     /label=ori
     terminator      2721..2752
                     /label=tonB_terminator
     CDS             complement(1721..2580)
                     /label=AmpR
     promoter        complement(2582..2685)
                     /label=AmpR_promoter
     terminator      809..856
                     /label=T7_terminator
     promoter        2600..2605
                     /label=minus_10
     promoter        complement(2344..2348)
                     /label=minus_10
     promoter        complement(778..782)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1533..1722
                     /label=spacer
     misc_feature    1537..1556
                     /label=anneal_sequence
     CDS             893..943
                     /label=spacer
     misc_feature    771..2771
                     /label=pBuild__pOpen_v3 backbone
     CDS             5..679
                     /label=eechGFP2
     misc_feature    1..4
                     /label=AATG
     misc_feature    680..683
                     /label=GCTT
ORIGIN
        1 AATGAACGTG ATCAAACCGG ACATGAAAAT CAAACTGCGT ATGGAAGGTG CCGTTAACGG
       61 CCATAAGTTT GCTATCGAAG GTGAAGGCAA CGGTCAGCCG TTTGAAGGCA AACAAACCAT
      121 GAATCTGAAA GTTAAAGAAG GCGGCCCGCT GCCGTTCGCT TACGATATCC TGACTACCAT
      181 TTTCAACTAC GGTAATCGCG TGTTTGTGAA ATACCCGGAT GACATTGTCG ACTACTTTAA
      241 ACAGTCATTT CCGGAAGGTT ATTCATGGGA GCGCAGCATG ATCTACGAGG ATGGCGGTAT
      301 TTGTATCGCT ACCAACGATA TCACCTTGGA AGGTGATTGC TTTGTATACA AAATCCGTTT
      361 CGACGGTGTG AACTTCCCGG CAAAGTCGCC GGTGCTGCAA AAGATGACCA AAAAATGGGA
      421 ACCGTCCACC GAAAAACTGT ACGTTCGTGA TGGTGTTCTG AAGGGCGATG TGAATATGGC
      481 ACTGCTCCTG GAGGGTGGCG GTCACTTCCG CTGCGATTTT AAAACCACCT ACAAAGCGAA
      541 GAAGGTTGTG CAGCTTCCGG ACTACCACTT TGTTGATCAT CGCATCGAAA TCATGAGCCA
      601 CGACAAAGAC TACAACAACG TAAAACTGTG CGAACACGCA GAAGCGCACA GCGGCCTGCC
      661 GGGCCAGGCG AAGTGAAGAG CTTTGAGACC GTGCTATATC TTCCTCCCGG CGCTATCTGC
      721 TGCTTCTGGG ATAGGGATAA CAGGGTAATT TTAAGCTAAG GCGTCTACCC CGCTGAGGTG
      781 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
      841 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
      901 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
      961 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1021 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1081 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1141 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1201 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1261 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1321 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1381 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1441 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1501 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     1561 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     1621 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     1681 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     1741 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     1801 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     1861 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     1921 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     1981 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2041 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2101 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2161 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2221 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2281 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2341 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     2401 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     2461 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     2521 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     2581 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     2641 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     2701 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG
     2761 TGGAGTGGGA GAAATAAGAC GACGACCCTC GGGTCTCT
//