LOCUS       ODC_0100                2810 bp    DNA     circular     29-Apr-2025
ACCESSION   ODC_0100
VERSION     ODC_0100.1  
FEATURES             Location/Qualifiers
     misc_feature    2804..2809
                     /label=BsaI_site
     misc_feature    complement(697..702)
                     /label=BsaI_site
     rep_origin      complement(954..1541)
                     /label=ori
     terminator      2733..2764
                     /label=tonB_terminator
     CDS             complement(1733..2592)
                     /label=AmpR
     promoter        complement(2594..2697)
                     /label=AmpR_promoter
     terminator      821..868
                     /label=T7_terminator
     promoter        2612..2617
                     /label=minus_10
     promoter        complement(2356..2360)
                     /label=minus_10
     promoter        complement(790..794)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1545..1734
                     /label=spacer
     misc_feature    1549..1568
                     /label=anneal_sequence
     CDS             905..955
                     /label=spacer
     misc_feature    783..2783
                     /label=pBuild__pOpen_v3 backbone
     promoter        163..168
                     /label=minus_35_pLlac_y_pLtet
     CDS             5..691
                     /label=eechGFP3
     misc_feature    1..4
                     /label=AATG
     misc_feature    692..695
                     /label=GCTT
ORIGIN
        1 AATGAACGTT ATTAAACCGG ACATGAAAAT CCGCCTGCGC ATGGAAGGTG CGGTGAACGG
       61 TCACAAATTC GTAATTATTG GTAAAGGCGA TGGCAAACCG TACGAAGGCA CCCAGACCAT
      121 GGATCTGGAG GTGATCGAAG GTGGTCCTCT GCCGTTCGCG TTTGACATTC TCACGACTGT
      181 TTTTAAATAT GGGAATCGTG CGTTCGTTAA ATACCCAACC GACATTGCCG ACTACTTCAA
      241 ACAGAGCTTC CCGGAGGGTT TCTCTTGGGA GCGTTCCATG ACCTACGAGG ACGGTGGCAT
      301 CTGCATCGCC ACCAACGATA TCACCCTGTC TAAAGATATT GCTAACTGCT TTGATTACAA
      361 CATTCGTTTC GACGGCGTTA ACTTCCCGCC GAACAGCCCT GTTCTGCAAA AAACTACCAT
      421 CAAATGGGAA CCGTCTACCG AAAACATGTA CGTACGTGAC GGTGTACTTA AAGGCGATAT
      481 CAATATGTCG CTCCTGTTGG AAGGTGGTGC AGGCCACTAC CGTTGCGACT TCAAAACGAC
      541 TTACAAAGCG AAAAAGGCCG TTAAACTGCC GGATTACCAC TTCGTTGATC ACCGTATTAC
      601 GATCGTTTCC CACGATAAAG ATTATAACAA AGTCAAACTG CGTGAACACG CAGAAGCTCA
      661 CAGCGGTCTG CAAATGGAAG CGAAGTGAAG AGCTTTGAGA CCCACTCGAT AGGTACAACC
      721 GGAGGGCAAC GGGAGCCATG CCTTAGGGAT AACAGGGTAA TCTCATTTGC GAAACTAGGG
      781 ACCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      841 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      901 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
      961 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1021 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1081 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1141 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1201 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1261 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1321 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1381 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1441 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1501 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1561 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     1621 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     1681 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     1741 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     1801 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     1861 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     1921 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     1981 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2041 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2101 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2161 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2221 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2281 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2341 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2401 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2461 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2521 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     2581 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     2641 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     2701 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     2761 ACTTGGTTCA GGTGGAGTGG GAGAAATAAG ACGACGACCC TCGGGTCTCT
//