LOCUS       ODC_0101                2786 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0101
VERSION     ODC_0101.1  
FEATURES             Location/Qualifiers
     misc_feature    2780..2785
                     /label=BsaI_site
     misc_feature    complement(673..678)
                     /label=BsaI_site
     rep_origin      complement(930..1517)
                     /label=ori
     terminator      2709..2740
                     /label=tonB_terminator
     CDS             complement(1709..2568)
                     /label=AmpR
     promoter        complement(2570..2673)
                     /label=AmpR_promoter
     terminator      797..844
                     /label=T7_terminator
     promoter        2588..2593
                     /label=minus_10
     promoter        complement(2332..2336)
                     /label=minus_10
     promoter        complement(766..770)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1521..1710
                     /label=spacer
     misc_feature    1525..1544
                     /label=anneal_sequence
     CDS             881..931
                     /label=spacer
     misc_feature    759..2759
                     /label=pBuild__pOpen_v3 backbone
     promoter        181..186
                     /label=minus_35_pLlac_y_pLtet
     CDS             5..667
                     /label=efasGFP
     misc_feature    1..4
                     /label=AATG
     misc_feature    668..671
                     /label=GCTT
ORIGIN
        1 AATGGCGCTC TCCAAACAGG GTATCCAGAC GGACATGAAA ATGACCTATA GCATGGAAGG
       61 TTGCGTGAAC GGTCACAACT TCACTGTGAA AGGTGGTGGC GACGGCAACC CGTATGAAGG
      121 TCATCAGGAA CTGCGTCTGT GTATCACCAT GGCTAAAGGC GAACCGGTGC CGTTCGCTTT
      181 TGACATCCTG TCCGCTGCAT TCTGCTACGG AAACCGCTGC TTCACTAAAT ATCCGGATGA
      241 AATTCGTGAC TACTTTAAAC AGGCATTCCC GGGCGGTCTG TCCTGGGAAC GTTCCATGGC
      301 ATTCGAGGAT GGGGCTTCCG CAGCCGTGAC TGCTGAGATC AGCCTGGAAG GCGATTGTTT
      361 CGAGCATGAA TGTGAATTTG TCGGCGTAAA CTTCCCTGCA AACGGTCCGG TTATGCAAAA
      421 GAAAACTCAG GGTTGGGAAA CTAGCACCGA AAAAATGACC GCGCACGGTA AAGTTGTGCA
      481 GGGCAATGTG CCTATGTTCC TGAAGCTGGA GGGCGGTGGT CGTCACCGTT GCGACTTCCG
      541 TACTACTTAT AAAGCTAAAA AAGATGTGAA AATGCCGAAC TCTCACTTTA TCACCCACTG
      601 CCTGGTGCGC AAAGGTGACG GTAACAACAC CGAACTGATC GAGGACGCGG AAGCACGTAA
      661 CTGAAGAGCT TTGAGACCCT TGAAATCTTG TCGCACGGAT TAGCCCTTCG CTCCGATGTT
      721 AGGGATAACA GGGTAATACA AATACGCCCA CGAGTCGGCG CTGAGGTGTC AATCGTCGGA
      781 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
      841 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
      901 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
      961 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1021 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1081 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1141 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1201 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1261 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1321 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1381 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     1441 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     1501 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT
     1561 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1621 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     1681 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     1741 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     1801 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     1861 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     1921 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     1981 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     2041 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     2101 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     2161 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     2221 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     2281 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     2341 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     2401 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     2461 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     2521 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     2581 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     2641 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC
     2701 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTTCAGGTG GAGTGGGAGA
     2761 AATAAGACGA CGACCCTCGG GTCTCT
//