LOCUS       ODC_0102                2801 bp    DNA     circular     17-Jun-2025
ACCESSION   ODC_0102
VERSION     ODC_0102.1  
FEATURES             Location/Qualifiers
     misc_feature    2795..2800
                     /label=BsaI_site
     misc_feature    complement(688..693)
                     /label=BsaI_site
     rep_origin      complement(945..1532)
                     /label=ori
     terminator      2724..2755
                     /label=tonB_terminator
     CDS             complement(1724..2583)
                     /label=AmpR
     promoter        complement(2585..2688)
                     /label=AmpR_promoter
     terminator      812..859
                     /label=T7_terminator
     promoter        2603..2608
                     /label=minus_10
     promoter        complement(2347..2351)
                     /label=minus_10
     promoter        complement(781..785)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1536..1725
                     /label=spacer
     misc_feature    1540..1559
                     /label=anneal_sequence
     CDS             896..946
                     /label=spacer
     misc_feature    774..2774
                     /label=pBuild__pOpen_v3 backbone
     CDS             5..682
                     /label=gfasGFP
     misc_feature    1..4
                     /label=AATG
     misc_feature    683..686
                     /label=GCTT
ORIGIN
        1 AATGAACGTT ATTAAACCGG ACATGAAAAT CAAACTGTGC ATGCGTGGCA CCATTAATGG
       61 TCACAACTTC GTGATCGAGG GGGAAGGTAA AGGCAACCCA TACGAAGGTA CCCAGATCCT
      121 GGATCTGAAC GTGACCGAAG GCGCACCGCT GCCGTTCGCA TATGACATTC TGACCACTGT
      181 ATTTCAGTAC GGTAACCGCG CATTTACCAA ATACCCGGCC GACATCCAAG ACTACTTCAA
      241 GCAGACTTTC CCGGAAGGCT ATCACTGGGA ACGTTCCATG ACTTACGAAG ATCAGGGTAT
      301 CTGTACTGCC ACCAGCAACA TCTCCATGCG TGGTGACTGT TTCTTCTACG ACATTCGCTT
      361 TGACGGTGTG AACTTTCCGC CGAACGGTCC GGTCATGCAG AAGAAAACCC TCAAATGGGA
      421 ACCGTCCACA GAAAAGATGT ACGTGCGTGA TGGTGTGCTG AAGGGTGATG TGAACATGGC
      481 TCTGCTGCTG GAGGGCGGCG GCCACTACCG CTGCGATTTC AAGACTACTT ACAAAGCTAA
      541 AAAGGATGTC CGTTTACCGG ACTACCACTT CGTGGACCAC CGTATCGAAA TTCTGAAACA
      601 CGATAAAGAT TACAATAAAG TAGAACTGTA CGAAAATGCG GTGGCCCGCT ACAGCATGCT
      661 GCCGTCCCAG GCGAAGTGAA GAGCTTTGAG ACCCCGAGGG GTCAGTAATT CTTTGGAAAT
      721 ATCAGTAGTT AAACTAGGGA TAACAGGGTA ATACAACTAA GCCTGGACCA GATCGCTGAG
      781 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      841 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
      901 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
      961 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1021 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1081 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1141 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1201 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1261 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1321 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1381 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1441 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1501 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     1561 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     1621 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     1681 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     1741 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     1801 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     1861 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     1921 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     1981 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2041 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2101 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2161 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2221 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2281 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2341 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2401 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     2461 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     2521 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     2581 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     2641 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     2701 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC
     2761 AGGTGGAGTG GGAGAACGAT GATGCTCACT CTCGGGTCTC T
//