LOCUS       ODC_0103                2816 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0103
VERSION     ODC_0103.1  
FEATURES             Location/Qualifiers
     misc_feature    2810..2815
                     /label=BsaI_site
     misc_feature    complement(703..708)
                     /label=BsaI_site
     rep_origin      complement(960..1547)
                     /label=ori
     terminator      2739..2770
                     /label=tonB_terminator
     CDS             complement(1739..2598)
                     /label=AmpR
     promoter        complement(2600..2703)
                     /label=AmpR_promoter
     terminator      827..874
                     /label=T7_terminator
     promoter        2618..2623
                     /label=minus_10
     promoter        complement(2362..2366)
                     /label=minus_10
     promoter        complement(796..800)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1551..1740
                     /label=spacer
     misc_feature    1555..1574
                     /label=anneal_sequence
     CDS             911..961
                     /label=spacer
     misc_feature    789..2789
                     /label=pBuild__pOpen_v3 backbone
     promoter        172..177
                     /label=minus_35_pLlac_y_pLtet
     CDS             5..697
                     /label=plamGFP
     misc_feature    1..4
                     /label=AATG
     misc_feature    698..701
                     /label=GCTT
ORIGIN
        1 AATGGAGATC GAGGATGTGA TCAAACGCGA CATGAAAATC AAGCTGCGTA TGGAAGGTGC
       61 TGTTAACGGT CACAAATTCG TGATCATTGG TAACGGCGAT GGCAAACCAT ATGAAGGCAC
      121 TCAGACCATC GATCTGGAAG TCATTGAAGG TGCCCCGCTG CCGTTCGCAT TTGACATCCT
      181 GACTACAGTT TTTGAATACG GTAACAGAGT TTTCGCCAAA TATCCGAACG AAATTGTTGA
      241 CTACTTCAAA CAATCCTTTC CGGAGGGCTA CTCTTGGGAG CGTTCTATGA CCTACGAGGA
      301 CGGCGGCATT TGTATCGCCA CCAACAATAT CACCCTGTCC AAAGTAATCG ATAACTGCTT
      361 CCATTACAAC ATCCGTTTCG ATGGGGTGAA TTTCCCGCCG AAAAGCCCGG TGCTTCAGAA
      421 AACCACCATG AAATGGGAAC CGTCCACCGA AATCATGTAC GTTCGTGACG GTGTGCTGAA
      481 AGGCGACGTA AACATGGCCC TGCTGATCGA AGGCGGTGGT CATTATCGTT GCGATTTTAA
      541 AACGATCTAC AAAGCGAAAA AAGTTGTTGA ACTGCCGGAC TATCACTTTG TCGACCACCG
      601 TATCACCATT AAATCACACG ACAAAGACTA CAACAAAGTC CTGCTTCACG AACACGCAAA
      661 AGCCCGTTAC GGCCTCCAGC GTAAAGCGAA GTGAAGAGCT TTGAGACCGT AATGGCTCGG
      721 ATCGTCTTGT TACTCATCCT AACACGACGT AGGGATAACA GGGTAATAGA ATTTTAAAAG
      781 ACTTCTCCCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
      841 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
      901 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
      961 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1021 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1081 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1141 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1201 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1261 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1321 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1381 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1441 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1501 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     1561 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     1621 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     1681 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     1741 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     1801 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     1861 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     1921 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     1981 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2041 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2101 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2161 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2221 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2281 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2341 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     2401 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     2461 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     2521 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     2581 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     2641 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     2701 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     2761 CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCT
//