LOCUS       ODC_0104                2822 bp    DNA     circular     17-Jun-2025
ACCESSION   ODC_0104
VERSION     ODC_0104.1  
FEATURES             Location/Qualifiers
     misc_feature    2816..2821
                     /label=BsaI_site
     misc_feature    complement(709..714)
                     /label=BsaI_site
     rep_origin      complement(966..1553)
                     /label=ori
     terminator      2745..2776
                     /label=tonB_terminator
     CDS             complement(1745..2604)
                     /label=AmpR
     promoter        complement(2606..2709)
                     /label=AmpR_promoter
     terminator      833..880
                     /label=T7_terminator
     promoter        2624..2629
                     /label=minus_10
     promoter        complement(2368..2372)
                     /label=minus_10
     promoter        complement(802..806)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1557..1746
                     /label=spacer
     misc_feature    1561..1580
                     /label=anneal_sequence
     CDS             917..967
                     /label=spacer
     misc_feature    795..2795
                     /label=pBuild__pOpen_v3 backbone
     promoter        544..549
                     /label=minus_35_pLlac_y_pLtet
     CDS             5..703
                     /label=amilRFP
     misc_feature    1..4
                     /label=AATG
     misc_feature    704..707
                     /label=GCTT
ORIGIN
        1 AATGGCGCTG TCCAAACACG GCTTGACTAA AGATATGACG ATGAAATACC ACATGGAAGG
       61 TTCAGTTGAC GGTCACAAGT TCGTTATTAC CGGGCACGGT AACGGTAACC CATTCGAAGG
      121 TAAACAAACC ATGAACCTGT GCGTGGTAGA AGGTGGTCCG TTGCCGTTCA GCGAGGACAT
      181 TCTGTCGGCG GCTTTCGATT ACGGTAACCG TGTGTTCACC GAATACCCGC AGGGTATGGT
      241 TGATTTCTTC AAAAACTCCT GCCCGGCCGG TTACACTTGG CACCGTTCTC TGCTTTTCGA
      301 AGATGGTGCG GTGTGCACTA CCTCGGCAGA TATTACGGTT TCGGTGGAGG AAAACTGCTT
      361 CTATCATAAC TCTAAGTTCC ATGGCGTGAA CTTTCCGGCC GACGGTCCAG TTATGAAGAA
      421 AATGACGACC AACTGGGAAC CATCCTGCGA AAAGATTATC CCGGTTCCGC GTCAGGGCAT
      481 TCTGAAGGGC GACATCGCTA TGTACCTGCT GCTGAAGGAC GGTGGTCGTT ACCGTTGCCA
      541 GTTTGACACC ATTTATAAAG CTAAAAGCGA CCCGAAAGAA ATGCCGGAAT GGCACTTTAT
      601 TCAGCACAAA CTGACGCGTG AGGACCGCAG TGATGCGAAA AACCAAAAAT GGCAACTCGT
      661 GGAGCACGCT GTCGCATCTC GCTCCGCACT GCCGGGCTGA AGAGCTTTGA GACCCAACCT
      721 GGTATGCAGT CCTTCTAGAT TAGCACTCCA TAGACTAGGG ATAACAGGGT AATCGATACC
      781 AATGATTTCG ATGACGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
      841 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
      901 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
      961 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1021 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1081 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1141 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1201 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1261 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1321 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1381 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1441 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1501 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG
     1561 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     1621 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     1681 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     1741 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     1801 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     1861 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     1921 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     1981 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     2041 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     2101 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     2161 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     2221 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     2281 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     2341 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     2401 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     2461 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     2521 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     2581 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     2641 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     2701 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC
     2761 CGGAGGCTTT TGACTTGGTT CAGGTGGAGT GGGAGAACGA TGATGCTCAC TCTCGGGTCT
     2821 CT
//