LOCUS       ODC_0105                2828 bp    DNA     circular     29-Apr-2025
ACCESSION   ODC_0105
VERSION     ODC_0105.1  
FEATURES             Location/Qualifiers
     misc_feature    2822..2827
                     /label=BsaI_site
     misc_feature    complement(715..720)
                     /label=BsaI_site
     rep_origin      complement(972..1559)
                     /label=ori
     terminator      2751..2782
                     /label=tonB_terminator
     CDS             complement(1751..2610)
                     /label=AmpR
     promoter        complement(2612..2715)
                     /label=AmpR_promoter
     terminator      839..886
                     /label=T7_terminator
     promoter        2630..2635
                     /label=minus_10
     promoter        complement(2374..2378)
                     /label=minus_10
     promoter        complement(808..812)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1563..1752
                     /label=spacer
     misc_feature    1567..1586
                     /label=anneal_sequence
     CDS             923..973
                     /label=spacer
     misc_feature    801..2801
                     /label=pBuild__pOpen_v3 backbone
     promoter        complement(538..542)
                     /label=minus_35_pLlac_y_pLtet
     CDS             5..709
                     /label=meffRed_meffRFP__Biobricks_K592012
     misc_feature    1..4
                     /label=AATG
     misc_feature    710..713
                     /label=GCTT
ORIGIN
        1 AATGGCACTG AGTAAAAATG GCCTGACCAA AAACATGACG ACCAAATATC GTATGGAAGG
       61 TTGCGTGGAC GGTCACAAGT TCGTTATCAC CGGCGATGGT ATTGGCGACC CGTTTGAAGG
      121 TAAACAGACG AGCATCGATC TGTGCGTGGT TGAAGGCGGT CCGCTGCCGT TCAGTGAAGA
      181 TATTCTGTCC GCAGTTTTTG ACTATGGCAA CCGTGTTTTC ACCAAATATC CGCAGGATCT
      241 GGTTGACTAC TTTAAGAATA GCTGTCCGGC GGGTTACACC TGGCAACGCT CTTTTCTGTT
      301 CGAGGACGGT GCAGTCTGCA CCGCGTCAGC CGATATTACG GTTTCGGTGG AAGAAAACTG
      361 TTTCTACCAT GAAAGCAAGT TTCACGGTGT GAATTTCCCG GCCGATGGCC CGGTTATGAA
      421 AAAGATGACC ACGAATTGGG AACCGTCTTG CGAAAAAATT ACCCCGATCC CGAATGAAGG
      481 TATCCTGAAA GGCGACGTCA CGATGTTTCT GCTGCTGAAG GATGGCGGTC GTTATCGCTG
      541 TCAATTCGAT ACCGTGTACA AAGCAAAGAG TGACCCGAAA ACGATTATGA TGCCGGATTG
      601 GCATTTTATC CAGCACAAGC TGAACCGTGA AGATCGCTCC GACGCTAAAC ATCAAAAGTG
      661 GCGTCTGGTG GAAAATGCAA TCGCTTACCG CAGCACCCTG TCCTGAAGAG CTTTGAGACC
      721 GGCGGTGTCT ACATATCCTT TCAATCGCTC TCGACCGGCG ATAGGGATAA CAGGGTAATA
      781 GAGCGACTCT TCCCCGGTAA CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
      841 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
      901 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
      961 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1021 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1081 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1141 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1201 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1261 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1321 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1381 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1441 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1501 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1561 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1621 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     1681 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     1741 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     1801 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     1861 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     1921 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     1981 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2041 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2101 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2161 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2221 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2281 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2341 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2401 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2461 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2521 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2581 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     2641 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     2701 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     2761 TCCGACCGGA GGCTTTTGAC TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC
     2821 GGGTCTCT
//