LOCUS       ODC_0107                2804 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0107
VERSION     ODC_0107.1  
FEATURES             Location/Qualifiers
     misc_feature    2798..2803
                     /label=BsaI_site
     misc_feature    complement(691..696)
                     /label=BsaI_site
     rep_origin      complement(948..1535)
                     /label=ori
     terminator      2727..2758
                     /label=tonB_terminator
     CDS             complement(1727..2586)
                     /label=AmpR
     promoter        complement(2588..2691)
                     /label=AmpR_promoter
     terminator      815..862
                     /label=T7_terminator
     promoter        2606..2611
                     /label=minus_10
     promoter        complement(2350..2354)
                     /label=minus_10
     promoter        complement(784..788)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1539..1728
                     /label=spacer
     misc_feature    1543..1562
                     /label=anneal_sequence
     CDS             899..949
                     /label=spacer
     misc_feature    777..2777
                     /label=pBuild__pOpen_v3 backbone
     promoter        complement(665..669)
                     /label=minus_10
     CDS             5..685
                     /label=meleRFP
     misc_feature    1..4
                     /label=AATG
     misc_feature    686..689
                     /label=GCTT
ORIGIN
        1 AATGAGCGTT ATTAAGCCGG ACATGAAGCT GAAACTGCGT ATGGAAGGCA CTGTTAATGG
       61 CCACTACTTC GTGATTGAAG GCGATGGTAA GGGCCGTCCG TTTGAGGGCA AACAGAGCAT
      121 GGATCTGGAC GTAAAAGAGG GTGGTCCTCT GCCGTTCGCT TACGACATCC TGACCACCGC
      181 GTTCCATTAC GGTAACCGTG TTTTCGCGGA ATATCCGGAT CACATCCCGG ACTATTTTAA
      241 GCAGTCCTTC CCGGGTGGTT ATTCTTGGGA ACGATCCCTG ACCTTCGAAG ATGGCGGCAT
      301 CTGCATCGCA CGTAACGATA TCAAAATGGT GGGCGACACC TTCTACAACA CTGTACGTTT
      361 CGATGGCGTT AACTTCCCTC CGAACGGCCC GGTTATGCAG CGCCGCACCC AGAAATGGGA
      421 GCCGTCTACC GAGAAAATCT ATGTACGTGA CGGCGTACTG ACTGGTGATA TTACCATGGC
      481 GTTGCTGCTG GAAGGTGGTG TTCACTATCG TTGCGACTTC CGTACTACTT ATAAAGCGAA
      541 AGAAAAAGGT GTACAGCTGC CTGGCTATCA TTTCGTTGAC CATTGCATCG AAATCCTGAG
      601 CCACGACAAA GACTACAACA AGGTTAAGCT GTACGAACAC GCTGTTGCGC ACTCTGGCCT
      661 GCCGGATAAT AAGCGTCAGT GAAGAGCTTT GAGACCGAGT ATTATCCGAG CGCCTTAAAT
      721 ATATGTGAAT ATCCGATTAG GGATAACAGG GTAATAGCAG CAGGGATAAT CATTTCCGCT
      781 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      841 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
      901 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
      961 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1021 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1081 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1141 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1201 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1261 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1321 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1381 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1441 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1501 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1561 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1621 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1681 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1741 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     1801 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     1861 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     1921 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     1981 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2041 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2101 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2161 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2221 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2281 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2341 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2401 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2461 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2521 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2581 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2641 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2701 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     2761 TTCAGGTGGA GTGGGAGAAC GATGATGCTC ACTCTCGGGT CTCT
//