LOCUS       ODC_0108                2816 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0108
VERSION     ODC_0108.1  
FEATURES             Location/Qualifiers
     misc_feature    2810..2815
                     /label=BsaI_site
     misc_feature    complement(703..708)
                     /label=BsaI_site
     rep_origin      complement(960..1547)
                     /label=ori
     terminator      2739..2770
                     /label=tonB_terminator
     CDS             complement(1739..2598)
                     /label=AmpR
     promoter        complement(2600..2703)
                     /label=AmpR_promoter
     terminator      827..874
                     /label=T7_terminator
     promoter        2618..2623
                     /label=minus_10
     promoter        complement(2362..2366)
                     /label=minus_10
     promoter        complement(796..800)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1551..1740
                     /label=spacer
     misc_feature    1555..1574
                     /label=anneal_sequence
     CDS             911..961
                     /label=spacer
     misc_feature    789..2789
                     /label=pBuild__pOpen_v3 backbone
     promoter        complement(63..67)
                     /label=minus_35_pLlac_y_pLtet
     CDS             5..697
                     /label=scubRFP
     misc_feature    1..4
                     /label=AATG
     misc_feature    698..701
                     /label=GCTT
ORIGIN
        1 AATGTCTGCG ATCAAAACCG TTGTTAAACA GTTCATGAAA ATCAAAATGT CTCTGGAGGG
       61 TACTGTCAAC GGCCACTACT TCAAAATTGT CGGTGAGGGC GACGGCACCC CGTTCGAGGG
      121 TAAACAGACT CTTCATCTGA AAGTCAAAGA AGGAGCCCCG CTGCCTTTTG CTTACGATAT
      181 TTTAACCACT GCGCTGCATT ACGGGAACCG TGTTTTCGTC GAATACCCGG AGAACATTCC
      241 AGACTATTTC AAACAGTCGT TCCCGAAAGG TTACTCTTGG GAACGCAGCC TGACCTTCGA
      301 AGATGGTGGC ATCTGTATCG CCCGTTCTGA CATCAAGATG GTGGGCGATA CCTTCCATAA
      361 CGAAGTTCAG TTCTATGGCG TTAACTTCCC GGCGAACGGT CCGGTTATGC AGCGTCACAC
      421 CGTTAAATGG GAGCCGAGCA CCGAGAAAAT CTACGTTCGT GACGGTGTGC TGACCGGTGA
      481 TATTACTATG GCACTGCTGC TCAAAGGTGG TACTCACTAC CGTTGCGACT TCCGCACCAC
      541 CTATAAAGCG AAAGAAAAAG GTCCGAAATT CCCGGGTTAC CACCTGGTGG ATCACTGTAT
      601 CGAGATTACT TCTCACGATA AAGATTATAA CGTGGTTGAA CTGTACGAAC ACGCTGTGGC
      661 TCACTCCGGT CTGCCGGACT CCGCGAACCG CTGAAGAGCT TTGAGACCGC GGTCGCTATA
      721 CATACCTTTT GCCCCGCTTA AACTATACCT AGGGATAACA GGGTAATCCG TTGGCTCAGC
      781 GTCGATGCCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
      841 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
      901 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
      961 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1021 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1081 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1141 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1201 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1261 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1321 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1381 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1441 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1501 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     1561 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     1621 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     1681 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     1741 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     1801 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     1861 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     1921 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     1981 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2041 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2101 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2161 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2221 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2281 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2341 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     2401 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     2461 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     2521 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     2581 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     2641 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     2701 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     2761 CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCT
//