LOCUS       ODC_0109                2807 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0109
VERSION     ODC_0109.1  
FEATURES             Location/Qualifiers
     misc_feature    2801..2806
                     /label=BsaI_site
     misc_feature    complement(694..699)
                     /label=BsaI_site
     rep_origin      complement(951..1538)
                     /label=ori
     terminator      2730..2761
                     /label=tonB_terminator
     CDS             complement(1730..2589)
                     /label=AmpR
     promoter        complement(2591..2694)
                     /label=AmpR_promoter
     terminator      818..865
                     /label=T7_terminator
     promoter        2609..2614
                     /label=minus_10
     promoter        complement(2353..2357)
                     /label=minus_10
     promoter        complement(787..791)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1542..1731
                     /label=spacer
     misc_feature    1546..1565
                     /label=anneal_sequence
     CDS             902..952
                     /label=spacer
     misc_feature    780..2780
                     /label=pBuild__pOpen_v3 backbone
     CDS             5..688
                     /label=eforRed_eforCP_Biobrick_K592012
     misc_feature    1..4
                     /label=AATG
     misc_feature    689..692
                     /label=GCTT
ORIGIN
        1 AATGTCCGTG ATTAAACAGG TGATGAAAAC CAAACTGCAC TTGGAAGGCA CCGTGAACGG
       61 TCACGATTTC ACCATCGAGG GTAAAGGTGA AGGTAAACCG TATGAAGGTC TGCAACACAT
      121 GAAAATGACT GTAACTAAAG GTGCCCCGCT CCCGTTTTCC GTCCACATCC TGACTCCGTC
      181 CCACATGTAC GGTAGCAAGC CGTTCAATAA ATATCCAGCG GATATCCCGG ACTATCACAA
      241 GCAGTCTTTC CCGGAAGGCA TGAGCTGGGA ACGTTCTATG ATCTTCGAAG ATGGCGGTGT
      301 GTGCACCGCT TCCAACCACT CTTCTATCAA CCTCCAGGAA AATTGTTTTA TCTACGATGT
      361 GAAATTTCAT GGTGTTAACC TGCCGCCGGA TGGCCCGGTA ATGCAGAAAA CCATCGCAGG
      421 TTGGGAACCG AGCGTGGAAA CGCTGTACGT GCGTGACGGT ATGCTGAAAT CTGACACTGC
      481 AATGGTATTC AAACTTAAGG GGGGTGGTCA CCACCGTGTT GATTTCAAGA CTACCTACAA
      541 AGCTAAAAAA CCGGTAAAAC TGCCGGAATT TCACTTCGTT GAACACCGTT TGGAACTGAC
      601 CAAGCACGAT AAAGACTTCA CGACTTGGGA CCAGCAAGAA GCTGCGGAAG GCCATTTCTC
      661 CCCTCTGCCG AAAGCACTGC CATGAAGAGC TTTGAGACCC CGCGTAAGAG GTTCCTATTT
      721 GTCTCAGTGG TTGTGAAGGC TAGGGATAAC AGGGTAATAT CCGTAGCCAT CTCTCGCAGC
      781 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
      841 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
      901 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
      961 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1021 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1081 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1141 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1201 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1261 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1321 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1381 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1441 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1501 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     1561 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     1621 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     1681 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     1741 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     1801 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     1861 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     1921 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     1981 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2041 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2101 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2161 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2221 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2281 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2341 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2401 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     2461 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     2521 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     2581 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     2641 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     2701 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     2761 TGGTTCAGGT GGAGTGGGAG AACGATGATG CTCACTCTCG GGTCTCT
//