LOCUS       ODC_0111                2825 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0111
VERSION     ODC_0111.1  
FEATURES             Location/Qualifiers
     misc_feature    2819..2824
                     /label=BsaI_site
     misc_feature    complement(712..717)
                     /label=BsaI_site
     rep_origin      complement(969..1556)
                     /label=ori
     terminator      2748..2779
                     /label=tonB_terminator
     CDS             complement(1748..2607)
                     /label=AmpR
     promoter        complement(2609..2712)
                     /label=AmpR_promoter
     terminator      836..883
                     /label=T7_terminator
     promoter        2627..2632
                     /label=minus_10
     promoter        complement(2371..2375)
                     /label=minus_10
     promoter        complement(805..809)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1560..1749
                     /label=spacer
     misc_feature    1564..1583
                     /label=anneal_sequence
     CDS             920..970
                     /label=spacer
     misc_feature    798..2798
                     /label=pBuild__pOpen_v3 backbone
     CDS             5..706
                     /label=EiraCFP__Biobrick_J97000
     misc_feature    1..4
                     /label=AATG
     misc_feature    707..710
                     /label=GCTT
ORIGIN
        1 AATGTCGTCT GGTACCAAAT TGTTTGAAAA GGAAATCCCG TATATCACTG AACTGGAGGG
       61 CGACGTCAAT GGTATGAAGT TTACCATTCA TGGTAAAGGT ACCGGCGATG CGAGCACGGG
      121 TACCATTAAA GCGAAATTCA TCTGCACTAC GGGCGACGTT CCGGTCCCGT GGGCAACCCT
      181 GGTGAGCACC CTGAGCTACG GTGTTCAGTG TTTCGCCAAG TACCCGCCGC ACATCAAGGA
      241 TTTCTTTAAG AGCGCCATGC CGGAAGGTTA TGTTCAAGAG CGTACCATCA CCTTCGAAGG
      301 CGACGGCGTG TTTAAGACGC GTGCTGAGGT TACCTTTGAA AACGGTTCTG TCTACAATCG
      361 TGTCACGCTG AACGGCCAGG GTTTTAAGAA AGACGGTCAC ATTCTGCGTA AGAACGTTGC
      421 ATTCCAATGC CCGCCAGATA TTGTGTATAT TTTGCCTGAC ACCGTTAACA ATGGCATCCG
      481 CGTTGAGTTC AACCAGGCGT ACGATATTGA AGGTGTGACC GAAAAACTGG TTACCAAATG
      541 CAGCCAAATG AATCGTCCGT TGGCGGGCTC CGCGGCAGTG CATATCCCGC GTTATCATCA
      601 CCTGAGCAAA CACACCAAAC TGAGCAAAGA CCGCGACGAG CGCCGTGATC ACATGTGTCT
      661 GGTAGAGGTC GTGAAAGCGG TTGATCTGGA CACGTATCAG TGAAGAGCTT TGAGACCCGC
      721 GCTAATAGCT TTGCATTGCC TGCGTATATC TTTCTTTGTA GGGATAACAG GGTAATGTAC
      781 CATTGAAAAG CTCGCTTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
      841 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
      901 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
      961 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1021 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1081 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1141 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1201 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1261 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1321 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1381 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1441 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1501 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1561 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1621 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1681 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     1741 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     1801 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     1861 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     1921 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     1981 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2041 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2101 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2161 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2221 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2281 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2341 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2401 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2461 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2521 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2581 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2641 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     2701 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     2761 GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAGAA CGATGATGCT CACTCTCGGG
     2821 TCTCT
//