LOCUS       ODC_0112                3185 bp    DNA     circular     30-Apr-2025
ACCESSION   ODC_0112
VERSION     ODC_0112.1  
FEATURES             Location/Qualifiers
     misc_feature    3179..3184
                     /label=BsaI_site
     misc_feature    complement(1072..1077)
                     /label=BsaI_site
     rep_origin      complement(1329..1916)
                     /label=ori
     terminator      3108..3139
                     /label=tonB_terminator
     CDS             complement(2108..2967)
                     /label=AmpR
     promoter        complement(2969..3072)
                     /label=AmpR_promoter
     terminator      1196..1243
                     /label=T7_terminator
     promoter        2987..2992
                     /label=minus_10
     promoter        complement(2731..2735)
                     /label=minus_10
     promoter        complement(1165..1169)
                     /label=minus_35_pLlac_y_pLtet
     misc_feature    1920..2109
                     /label=spacer
     misc_feature    1924..1943
                     /label=anneal_sequence
     CDS             1280..1330
                     /label=spacer
     misc_feature    1158..3158
                     /label=pBuild__pOpen_v3 backbone
     CDS             5..1066
                     /label=HRP
     misc_feature    1..4
                     /label=AATG
     misc_feature    1067..1070
                     /label=GCTT
ORIGIN
        1 AATGCACTTC AGCTCTAGCA GTACCCTGTT TACCTGTATC ACCCTGATCC CGCTGGTATG
       61 CCTGATCCTG CATGCGAGCC TGAGCGACGC GCAGCTGACT CCGACCTTCA TCGACAACTC
      121 CTGCCCGAAC GTCAGTAACA TCGTGCGTGA CACCATCGTC AACGAACTGC GCTCCGATCC
      181 GCGTATCGCA GCTTCCATCC TGCGTCTGCA CTTCCATGAC TGTTTCGTGA ACGGGTGCGA
      241 TGCGTCTATT CTGCTGGATA ACACCACCTC TTTTCGTACT GAAAAAGATG CGTTCGGTAA
      301 TGCTAACTCC GCACGTGGCT TTCCGGTGAT CGATCGCATG AAAGCTGCCG TCGAGTCTGC
      361 GTGTCCGCGT ACTGTTTCTT GCGCGGACCT GCTGACCATC GCCGCTCAGC AGTCCGTTAC
      421 TCTGGCTGGT GGTCCGTCTT GGCGCGTTCC ACTGGGTCGT CGTGATAGCC TGCAAGCATT
      481 TCTGGACCTG GCGAACGCGA ATCTCCCGGC TCCGTTCTTC ACCCTGCCGC AGCTGAAGGA
      541 CTCTTTCCGC AACGTTGGTC TGAACCGTTC TTCTGACCTG GTGGCGCTGA GCGGCGGCCA
      601 TACGTTTGGC AAAAACCAGT GTCGTTTCAT CATGGACCGC CTGTATAACT TCAGCAACAC
      661 GGGCCTGCCG GACCCGACCC TCAACACTAC GTACCTCCAA ACCCTGCGTG GGCTGTGCCC
      721 GCTGAACGGT AACTTATCCG CTCTGGTTGA CTTCGATCTG CGTACCCCGA CTATCTTCGA
      781 TAACAAGTAC TACGTTAATC TGGAAGAACA GAAAGGTCTG ATCCAGTCCG ACCAGGAACT
      841 GTTTTCTTCT CCGAACGCGA CCGATACCAT CCCGCTGGTG CGGTCGTTTG CTAACTCCAC
      901 CCAGACTTTC TTTAACGCCT TCGTAGAGGC TATGGATCGT ATGGGCAATA TCACTCCACT
      961 GACTGGCACT CAGGGCCAGA TCCGTCTGAA CTGTCGTGTC GTTAACTCAA ACAGCCTGCT
     1021 CCACGACATG GTTGAAGTGG TTGACTTTGT CTCTTCTATG TGAAGAGCTT TGAGACCGAC
     1081 GACTGCCTAA GGACATTCCG GACATGTCGG AGGAAGAATA GGGATAACAG GGTAATGCTG
     1141 CACTTTCCGG GCTTCAACGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1201 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1261 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1321 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1381 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1441 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1501 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1561 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1621 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1681 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1741 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1801 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1861 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1921 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1981 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2041 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2101 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2161 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2221 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2281 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2341 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2401 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2461 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2521 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2581 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2641 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2701 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2761 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2821 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2881 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2941 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3001 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3061 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3121 GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAGAA CGATGATGCT CACTCTCGGG
     3181 TCTCT
//