LOCUS ODC_0117 3716 bp DNA circular 30-Apr-2025 ACCESSION ODC_0117 VERSION ODC_0117.1 FEATURES Location/Qualifiers misc_feature 3710..3715 /label=BsaI_site misc_feature complement(1603..1608) /label=BsaI_site rep_origin complement(1860..2447) /label=ori terminator 3639..3670 /label=tonB_terminator CDS complement(2639..3498) /label=AmpR promoter complement(3500..3603) /label=AmpR_promoter terminator 1727..1774 /label=T7_terminator promoter 3518..3523 /label=minus_10 promoter complement(3262..3266) /label=minus_10 promoter complement(1696..1700) /label=minus_35_pLlac_y_pLtet misc_feature 2451..2640 /label=spacer misc_feature 2455..2474 /label=anneal_sequence CDS 1811..1861 /label=spacer misc_feature 1689..3689 /label=pBuild__pOpen_v3 backbone promoter complement(546..550) /label=minus_35_pLlac_y_pLtet CDS 5..1597 /label=Calf_Intestine_Alkaline_phosphatase_CIAP misc_feature 1..4 /label=AATG misc_feature 1598..1601 /label=GCTT ORIGIN 1 AATGCAATGG GCCTGCGTTC TGCTGCTGCT GGGCCTGTGG CTCCAGCTGT CCCTGACCTT 61 CATCCCTGCC GAAGAAGAGG ACCCGGCGTT CTGGAATCGT CAGGCAGCGC AGGCTCTCGA 121 CGTAGCTAAA AAACTGCAAC CAATCCAGAC CGCAGCGAAA AACGTGATCC TGTTCCTGGG 181 TGACGGCATG GGTGTTCCTA CCGTTACCGC AACCCGTATC CTGAAAGGCC AGATGAACGG 241 CAAACTCGGC CCAGAAACCC CTCTGGCTAT GGATCAATTC CCGTACGTGG CGCTGAGCAA 301 AACCTACAAC GTTGACCGCC AAGTTCCGGA CTCTGCGGGC ACCGCGACTG CTTATTTGTG 361 TGGGGTTAAA GGCAACTACA AAACTATCGG TGTTTCTGCG GCAGCTCGTT ATAACCAGTG 421 CAACACCACC TCAGGTAACG AGGTAACCAG CGTGATGAAC CGTGCTAAAA AGGCCGGTAA 481 ATCCGTAGGC GTTGTTACGA CCTCCCGTGT TCAGCATGCA AGCCCGGCGG GTGCATACGC 541 GCACACTGTC AACCGTAACT GGTACAGCGA TGCGGATCTG CCGGCGGATG CACAGACCTA 601 CGGTTGCCAG GACATCGCAA CCCAGCTGGT TAATAACATG GACATCGACG TTATCCTGGG 661 TGGCGGTCGT ATGTACATGT TCCCGGAAGG CACCCCGGAC CCAGAATACC CGTACGACGT 721 AAACCAGACA GGTGTCCGTA AGGACAAACG TAACTTGGTC CAGGAGTGGC AAGCGAAGCA 781 CCAGGGTGCT CAGTACGTGT GGAACCGTAC CGAATTGCTG CAAGCTGCGA ATGATCCGTC 841 CGTTACGCAC CTGATGGGCC TCTTTGAACC TGCCGACATG AAATATAACG TACAGCAAGA 901 TCCGACTAAA GACCCGACTC TGGAGGAAAT GACTGAGGCT GCACTTCAGG TGCTGTCTCG 961 TAACCCGCAG GGTTTCTACC TGTTTGTAGA AGGTGGCCGT ATTGACCATG GTCACCACGA 1021 GGGTAAGGCA TACATGGCCC TGACCGATAC CGTCATGTTC GATAACGCCA TCGCCAAAGC 1081 AAACGAACTG ACATCAGAAC TGGATACCCT GATCCTGGCA ACCGCTGATC ACTCCCACGT 1141 GTTCTCTTTC GGCGGTTACA CGCTTCGCGG TACCTCCATC TTCGGTCTGG CTCCATCCAA 1201 AGCGTCTGAT AACAAGTCTT ACACCTCCAT CCTGTATGGT AACGGGCCGG GCTACGTGCT 1261 GGGTGGTGGT CTGCGTCCGG ATGTTAACGA CTCGATCAGC GAAGATCCGA GCTACCGTCA 1321 GCAGGCGGCG GTGCCTCTGT CCAGTGAATC TCACGGCGGC GAAGATGTGG CGGTTTTTGC 1381 ACGTGGCCCA CAGGCACACT TAGTCCACGG TGTGCAGGAA GAAACCTTTG TCGCGCACGT 1441 TATGGCTTTT GCAGGCTGCG TTGAACCGTA CACTGACTGT AACCTGCCGG CACCGTCTGG 1501 CCTGAGCGAC GCCGCACACC TGGCTGCCTC TCCGCCGAGC CTGGCCCTGC TGGCAGGTGC 1561 TATGCTGCTG CTTCTGGCGC CGGCTCTGTA CTGAAGAGCT TTGAGACCGG GCACTCAATA 1621 TACCCTGTTG TGCTCCGGTT TCTGCCCTGT AGGGATAACA GGGTAATGGT GACGCCTCGG 1681 ACGTGCTCCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT 1741 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT 1801 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA 1861 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 1921 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 1981 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 2041 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 2101 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 2161 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 2221 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 2281 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 2341 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 2401 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT 2461 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 2521 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 2581 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 2641 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 2701 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 2761 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 2821 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 2881 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 2941 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 3001 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 3061 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 3121 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 3181 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 3241 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 3301 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 3361 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 3421 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 3481 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 3541 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 3601 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG 3661 CTTTTGACTT GGTTCAGGTG GAGTGGGAGA ACGATGATGC TCACTCTCGG GTCTCT //