LOCUS       BBF10K_003332           2789 bp    DNA     circular     16-Jun-2025
ACCESSION   BBF10K_003332
VERSION     BBF10K_003332.1  
FEATURES             Location/Qualifiers
     CDS             5..670
                     /label=CE_meffBlue
     CDS             complement(1712..2571)
                     /label=AmpR
     promoter        complement(2573..2676)
                     /label=AmpR promoter
     primer_bind     2695..2711
                     /label=M13 fwd
     primer_bind     complement(850..865)
                     /label=M13 rev
     rep_origin      complement(933..1520)
                     /label=ColE1
     primer_bind     767..785
                     /label=SLiCE-for
     primer_bind     complement(2739..2756)
                     /label=SLiCE-rev
     misc_feature    1524..1713
                     /label=spacer
     CDS             884..934
                     /label=spacer
     terminator      800..847
                     /label=T7 terminator
     terminator      2712..2743
                     /label=tonB terminator
     misc_feature    762..2762
                     /label=pOpen_v3 backbone
     misc_feature    2783..2788
                     /label=BsaI_site
     misc_feature    complement(676..681)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    671..674
                     /label=GCTT
ORIGIN
        1 AATGTCCGTT ATCGCAACCC AGATGACGTA CAAAGTTTAT ATGTCGGGCA CCGTGAATGG
       61 TCATTATTTT GAAGTCGAAG GTGATGGCAA AGGTCGTCCG TATGAAGGCG AACAGACCGT
      121 CAAACTGACC GTGACGAAAG GCGGTCCGCT GCCGTTTGCA TGGGATATTC TGAGTCCGCA
      181 GTGCCAATAC GGTTCCATTC CGTTCACCAA ATATCCGGAA GATATCCCGG ACTACGTCAA
      241 ACAGAGCTTT CCGGAAGGTT TCACGTGGGA ACGCATTATG AACTTTGAAG ATGGCGCTGT
      301 GTGCACCGTT TCAAACGACA GCTCTATCCA AGGCAACTGC TTCACGTATC ATGTGAAATT
      361 TTCGGGTCTG AACTTCCCGC CGAATGGCCC GGTTATGCAG AAGAAAACCC AAGGTTGGGA
      421 ACCGCACAGT GAACGTCTGT TTGCGCGCGG CGGTATGCTG ATCGGCAACA ATTTCATGGC
      481 CCTGAAACTG GAAGGCGGTG GCCATTATCT GTGTGAATTT AAAACCACGT ACAAAGCGAA
      541 AAAACCAGTG AAAATGCCGG GTTATCATTA CGTTGATCGT AAACTGGACG TCACGAACCA
      601 CAATAAAGAC TATACCTCAG TTGAACAGTG TGAAATCAGC ATCGCACGCA AGCCGGTGGT
      661 CGCCTGAAGA GCTTTGAGAC CCGACTGCCG ACATGATCTA TGTAGACACG AAGGTCATTG
      721 CCTAGGGATA ACAGGGTAAT CGAGGACGAA ATTACGCTAC GCGCTGAGGT GTCAATCGTC
      781 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
      841 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
      901 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
      961 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1021 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1081 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1141 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1201 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1261 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1321 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1381 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1441 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1501 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     1561 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     1621 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     1681 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     1741 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     1801 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     1861 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     1921 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     1981 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2041 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2101 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2161 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2221 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2281 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     2341 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     2401 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     2461 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     2521 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     2581 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     2641 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     2701 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG
     2761 AGAAATAAGA CGACGACCCT CGGGTCTCT
//