BBF10K_003332_part 1 CE_meffBlue BBF10K_003498_backbone 1 pOpen_v3 backbone annotation3 1 M13 rev annotation3_range 1 89 104 annotation6 1 SLiCE-rev annotation6_range 1 1978 1995 annotation8 1 spacer annotation8_range 1 123 173 annotation10 1 tonB terminator annotation10_range 1 1951 1982 annotation0 1 AmpR annotation0_range 1 951 1810 annotation4 1 ColE1 annotation4_range 1 172 759 annotation9 1 T7 terminator annotation9_range 1 39 86 annotation7 1 spacer annotation7_range 1 763 952 annotation1 1 AmpR promoter annotation1_range 1 1812 1915 annotation2 1 M13 fwd annotation2_range 1 1934 1950 annotation5 1 SLiCE-for annotation5_range 1 6 24 BBF10K_003332 1 CE_meffBlue BBF10K_003332_part_comp 1 BBF10K_003498_comp 1 three_prime_fusion 1 GCTT three_prime_fusion_range 1 671 674 five_prime_fusion 1 AATG five_prime_fusion_range 1 1 4 BBF10K_003332_part_annot 1 BBF10K_003332_part_range 1 5 670 BBF10K_003498_annot 1 BBF10K_003498_range 1 762 2762 BsaI_site_rv_annotation 1 BsaI_site BsaI_site_rv_annotation_range 1 676 681 BsaI_site_fw_annotation 1 BsaI_site BsaI_site_fw_annotation_range 1 2783 2788 BBF10K_003332_part_seq 1 TCCGTTATCGCAACCCAGATGACGTACAAAGTTTATATGTCGGGCACCGTGAATGGTCATTATTTTGAAGTCGAAGGTGATGGCAAAGGTCGTCCGTATGAAGGCGAACAGACCGTCAAACTGACCGTGACGAAAGGCGGTCCGCTGCCGTTTGCATGGGATATTCTGAGTCCGCAGTGCCAATACGGTTCCATTCCGTTCACCAAATATCCGGAAGATATCCCGGACTACGTCAAACAGAGCTTTCCGGAAGGTTTCACGTGGGAACGCATTATGAACTTTGAAGATGGCGCTGTGTGCACCGTTTCAAACGACAGCTCTATCCAAGGCAACTGCTTCACGTATCATGTGAAATTTTCGGGTCTGAACTTCCCGCCGAATGGCCCGGTTATGCAGAAGAAAACCCAAGGTTGGGAACCGCACAGTGAACGTCTGTTTGCGCGCGGCGGTATGCTGATCGGCAACAATTTCATGGCCCTGAAACTGGAAGGCGGTGGCCATTATCTGTGTGAATTTAAAACCACGTACAAAGCGAAAAAACCAGTGAAAATGCCGGGTTATCATTACGTTGATCGTAAACTGGACGTCACGAACCACAATAAAGACTATACCTCAGTTGAACAGTGTGAAATCAGCATCGCACGCAAGCCGGTGGTCGCCTGAAGA BBF10K_003332_seq 1 AATGTCCGTTATCGCAACCCAGATGACGTACAAAGTTTATATGTCGGGCACCGTGAATGGTCATTATTTTGAAGTCGAAGGTGATGGCAAAGGTCGTCCGTATGAAGGCGAACAGACCGTCAAACTGACCGTGACGAAAGGCGGTCCGCTGCCGTTTGCATGGGATATTCTGAGTCCGCAGTGCCAATACGGTTCCATTCCGTTCACCAAATATCCGGAAGATATCCCGGACTACGTCAAACAGAGCTTTCCGGAAGGTTTCACGTGGGAACGCATTATGAACTTTGAAGATGGCGCTGTGTGCACCGTTTCAAACGACAGCTCTATCCAAGGCAACTGCTTCACGTATCATGTGAAATTTTCGGGTCTGAACTTCCCGCCGAATGGCCCGGTTATGCAGAAGAAAACCCAAGGTTGGGAACCGCACAGTGAACGTCTGTTTGCGCGCGGCGGTATGCTGATCGGCAACAATTTCATGGCCCTGAAACTGGAAGGCGGTGGCCATTATCTGTGTGAATTTAAAACCACGTACAAAGCGAAAAAACCAGTGAAAATGCCGGGTTATCATTACGTTGATCGTAAACTGGACGTCACGAACCACAATAAAGACTATACCTCAGTTGAACAGTGTGAAATCAGCATCGCACGCAAGCCGGTGGTCGCCTGAAGAGCTTTGAGACCCGACTGCCGACATGATCTATGTAGACACGAAGGTCATTGCCTAGGGATAACAGGGTAATCGAGGACGAAATTACGCTACGCGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAATAAGACGACGACCCTCGGGTCTCT BBF10K_003498_backbone_seq 1 CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAG