LOCUS       BBF10K_003356           2801 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003356
VERSION     BBF10K_003356.1  
FEATURES             Location/Qualifiers
     CDS             5..682
                     /label=CE_sarcGFP
     CDS             complement(1724..2583)
                     /label=AmpR
     promoter        complement(2585..2688)
                     /label=AmpR promoter
     primer_bind     2707..2723
                     /label=M13 fwd
     primer_bind     complement(862..877)
                     /label=M13 rev
     rep_origin      complement(945..1532)
                     /label=ColE1
     primer_bind     779..797
                     /label=SLiCE-for
     primer_bind     complement(2751..2768)
                     /label=SLiCE-rev
     misc_feature    1536..1725
                     /label=spacer
     CDS             896..946
                     /label=spacer
     terminator      812..859
                     /label=T7 terminator
     terminator      2724..2755
                     /label=tonB terminator
     misc_feature    774..2774
                     /label=pOpen_v3 backbone
     misc_feature    2795..2800
                     /label=BsaI_site
     misc_feature    complement(688..693)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=AATG
     misc_feature    683..686
                     /label=GCTT
ORIGIN
        1 AATGTCCGTG ATCAAACAGG AAATGAAAAT CAAGCTGCAC ATGGACGGCA ACGTTAACGG
       61 CCACGCGTTC GTAATCGAAG GCGATGGCAA AGGTAAACCG TACGATGGCA CCCAGACCCT
      121 GAACCTGAGC GTCAAAGAAG GTGCGCCGCT GCCGTTCTCC TATGATATTC TGACGAACGC
      181 GTTTCAGTAC GGCAATCGCG CCTTCACTAA ATACCCTGCG GACATCCCTG ATTACTTCAA
      241 ACAGACCTTT CCTGAGGGCT ATTCCTGGGA ACGTACCATG AGCTATGAAG ATAACGCCAT
      301 CTGCAACGTG CGTTCTGAAA TCTCCATGGA AGGCGACTGC TTCATCTACA AAATCCGTTT
      361 CGATGGCAAA AACTTCCCGC CGAACGGGCC GGTAATGCAA AAAAAGACCC TGAAATGGGA
      421 ACCGTCGACT GAAATGATGT ACGCTCGTGA CGGATTCCTT ATGGGCGATG TGAATATGGC
      481 GCTGCTGCTG GAAGGTGGTG GTCACCACCG TTGTGATTTC AAAACCTCCT ACAAGGCAGC
      541 GAAAAAAAAC GTACAGCTGC CTGACTACCA TTACGTCGAT CATCGTATTG AAATCCTGTC
      601 TCATGACCGT GACTACAACA AAGTGAAACT GTACGAAAAC GCAGTGGCTC GCTACAGCCT
      661 GCTGCCGAGC CAGGCCTGAA GAGCTTTGAG ACCGCTCGAT GCCATAGTTC CTTGGACGGT
      721 ATGAGGTTCA TGACTAGGGA TAACAGGGTA ATTAGTATAT GGTTTGGGCC GCGCGCTGAG
      781 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      841 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
      901 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
      961 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1021 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1081 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1141 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1201 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1261 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1321 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1381 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1441 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1501 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     1561 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     1621 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     1681 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     1741 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     1801 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     1861 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     1921 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     1981 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2041 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2101 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2161 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2221 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2281 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2341 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2401 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     2461 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     2521 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     2581 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     2641 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     2701 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC
     2761 AGGTGGAGTG GGAGAACGAT GATGCTCACT CTCGGGTCTC T
//