LOCUS BBF10K_003366 5192 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003366 VERSION BBF10K_003366.1 FEATURES Location/Qualifiers primer_bind complement(15..30) /label=M13 fwd CDS 5..3073 /label=CE_beta-galactosidase CDS complement(4115..4974) /label=AmpR promoter complement(4976..5079) /label=AmpR promoter primer_bind 5098..5114 /label=M13 fwd primer_bind complement(3253..3268) /label=M13 rev rep_origin complement(3336..3923) /label=ColE1 primer_bind 3170..3188 /label=SLiCE-for primer_bind complement(5142..5159) /label=SLiCE-rev misc_feature 3927..4116 /label=spacer CDS 3287..3337 /label=spacer terminator 3203..3250 /label=T7 terminator terminator 5115..5146 /label=tonB terminator misc_feature 3165..5165 /label=pOpen_v3 backbone misc_feature 5186..5191 /label=BsaI_site misc_feature complement(3079..3084) /label=BsaI_site misc_feature 1..4 /label=AATG misc_feature 3074..3077 /label=GCTT ORIGIN 1 AATGATTACG GATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT 61 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA 121 GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC 181 CTGGTTTCCG GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC 241 CGATACTGTC GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC 301 CAACGTGACC TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG 361 TTGTTACTCG CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT 421 TATTTTTGAT GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA 481 CGGCCAGGAC AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA 541 AAACCGCCTC GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA 601 TATGTGGCGG ATGAGCGGCA TTTTCCGTGA TGTCTCGTTG CTGCATAAAC CGACTACACA 661 AATCAGCGAT TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA 721 GGCTGAAGTT CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG 781 GCAGGGTGAA ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA 841 GCGTGGTGGT TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG 901 GAGCGCCGAA ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC 961 GCTGATTGAA GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT 1021 GCTGCTGCTG AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC 1081 TCTGCATGGT CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA 1141 GAACAACTTT AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT 1201 GTGCGACCGC TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT 1261 GCCAATGAAT CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC 1321 GCGAATGGTG CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA 1381 ATCAGGCCAC GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC 1441 CCGCCCGGTG CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC 1501 GATGTACGCG CGCGTGGATG AAGATCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA 1561 AAAATGGCTT TCGCTACCTG GAGAAACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC 1621 GATGGGTAAC AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG 1681 TTTACAGGGC GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA 1741 CGGCAACCCG TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT 1801 CTGTATGAAC GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA 1861 ACACCAGCAG CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA 1921 ATACCTGTTC CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA 1981 GCCGCTGGCA AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA 2041 ACTGCCTGAA CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT 2101 GCAACCGAAC GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG 2161 TCTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC 2221 CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA 2281 GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG 2341 CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT 2401 TGACCCTAAC GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC 2461 GTTGTTGCAG TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC 2521 GTGGCAGCAT CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG 2581 TGGTCAAATG GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG 2641 GATTGGCCTG AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG 2701 GCCGCAAGAA AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC 2761 ATTGTCAGAC ATGTATACCC CGTACGTCTT TCCGAGCGAA AACGGTCTGC GCTGCGGGAC 2821 GCGCGAATTG AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG 2881 CTACAGTCAA CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG 2941 CACATGGCTG AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC 3001 GTCAGTATCG GCGGAGTTCC AGCTGAGCGC CGGTCGCTAC CATTACCAGT TGGTCTGGTG 3061 TCAAAAGTGA AGAGCTTTGA GACCGCCGTG TAAAATGACG CATTGGAGAG TGTCCGAAAC 3121 GGACCTAGGG ATAACAGGGT AATATGCATG TCGGTTTAAC GGGACGCTGA GGTGTCAATC 3181 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 3241 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC 3301 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA 3361 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 3421 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 3481 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 3541 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 3601 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 3661 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 3721 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 3781 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 3841 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 3901 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC 3961 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 4021 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 4081 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 4141 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 4201 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA 4261 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 4321 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 4381 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 4441 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 4501 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 4561 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 4621 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 4681 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 4741 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 4801 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 4861 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 4921 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 4981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 5041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA 5101 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT 5161 GGGAGAACGA TGATGCTCAC TCTCGGGTCT CT //