LOCUS BBF10K_003373 2723 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003373 VERSION BBF10K_003373.1 FEATURES Location/Qualifiers CDS complement(1036..1895) /label=AmpR promoter complement(1897..2000) /label=AmpR promoter primer_bind 2019..2035 /label=M13 fwd primer_bind complement(174..189) /label=M13 rev rep_origin complement(257..844) /label=ColE1 primer_bind 91..109 /label=SLiCE-for primer_bind complement(2063..2080) /label=SLiCE-rev misc_feature 848..1037 /label=spacer CDS 208..258 /label=spacer terminator 124..171 /label=T7 terminator terminator 2036..2067 /label=tonB terminator misc_feature 5..2106 /label=AF_lacZ-pDest CDS complement(1036..1895) /label=AmpR promoter complement(1897..2000) /label=AmpR promoter primer_bind 2019..2035 /label=M13 fwd primer_bind complement(174..189) /label=M13 rev rep_origin complement(257..844) /label=ColE1 primer_bind 91..109 /label=SLiCE-for primer_bind complement(2063..2080) /label=SLiCE-rev misc_feature 848..1037 /label=spacer CDS 208..258 /label=spacer terminator 124..171 /label=T7 terminator terminator 2036..2067 /label=tonB terminator misc_feature 86..2086 /label=pOpen_v3 backbone misc_feature 2717..2722 /label=BsaI_site misc_feature complement(2112..2117) /label=BsaI_site misc_feature 1..4 /label=CGCT misc_feature 2107..2110 /label=GGAG ORIGIN 1 CGCTAGCCAC GGCGAAATTT AATGTGCACT TAATGCTCTA AGGAATTAGG GATAACAGGG 61 TAATAAGTGT GATCTATGAT TGTGACGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT 121 AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA 181 GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC 241 CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 301 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 361 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 421 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 481 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 541 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 601 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 661 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT 721 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 781 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 841 TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 901 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 961 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 1021 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 1081 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 1141 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG 1201 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 1261 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 1321 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 1381 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 1441 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 1501 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 1561 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 1621 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 1681 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 1741 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 1801 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 1861 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 1921 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 1981 ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA 2041 AAGCCTCCGA CCGGAGGCTT TTGACTTGGT TCAGGTGGAG TGGGAGAACG ATGATGCTCA 2101 CTCTCGGGAG CGAGACCATT GACAGCGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC 2161 GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT 2221 ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC 2281 AGGAAACAGC TATGACCATG ATTACGCCAA GCTTGCATGC CTGCAGGTCG ACTCTAGAGG 2341 ATCCCCGGGT ACCGAGCTCG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA 2401 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 2461 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT 2521 GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT 2581 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA 2641 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG 2701 TGACACAAGT GAGCACGGTC TCA //