LOCUS BBF10K_003377 5420 bp DNA circular 29-Apr-2025 ACCESSION BBF10K_003377 VERSION BBF10K_003377.1 FEATURES Location/Qualifiers misc_feature 5..3300 /label=AF_medium-copy-(pET)-ori/KanR CDS complement(4343..5202) /label=AmpR promoter complement(5204..5307) /label=AmpR promoter primer_bind 5326..5342 /label=M13 fwd primer_bind complement(3481..3496) /label=M13 rev rep_origin complement(3564..4151) /label=ColE1 primer_bind 3398..3416 /label=SLiCE-for primer_bind complement(5370..5387) /label=SLiCE-rev misc_feature 4155..4344 /label=spacer CDS 3515..3565 /label=spacer terminator 3431..3478 /label=T7 terminator terminator 5343..5374 /label=tonB terminator misc_feature 3393..5393 /label=pOpen_v3 backbone misc_feature 5414..5419 /label=BsaI_site misc_feature complement(3306..3311) /label=BsaI_site misc_feature 1..4 /label=CGCT misc_feature 3301..3304 /label=GGAG ORIGIN 1 CGCTAGCATG AAGAGCACCC CAGGATACAT AGATTACCAC AACTCCGAGC CCTTCCACCA 61 CTAGTCGCCG GCGAGGCCCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 121 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 181 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 241 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA 301 CAATTTCTGG CGGCACGATG GCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 361 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 421 GCTAGTATTA TTAGAAAAAC TCATCGAGCA TCAAATGAAA CTGCAATTTA TTCATATCAG 481 GATTATCAAT ACCATATTTT TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA AACTCACCGA 541 GGCAGTTCCA AAGAATGGCA AGGTCCTGGT AACGGTCTGC GATTCCGACC CGTCCAACAT 601 CAATACAACC TATTAATTTC CCCTCGTCAA AAATAAGGTT ATCAAGTGAG AAATCACCAT 661 GAGTGACGAC TGAATCCGGT GAGAATGGCA AGAGCTTGTG CATTTCTTTC CAGACTTGTT 721 CAACAGGCCA GCCATTACGC TCGTCATCAA AATCACTCGC ATCAACCAAA CCGTTATTCA 781 TGCGTGATTG CGCCTGAGCA AGACGAAATA CACGATCGCT GTTAAAAGGA CAATTACAAA 841 CAGGAATCGA ATGTAACCGG CGCAGGAACA CGGCCAGCGC ATCAACAATA TTTTCACCTG 901 AATCAGGATA TTCTTCTAAT ACCTGGAAGG CTGTTTTCCC AGGAATCGCG GTGGTGAGTA 961 ACCACGCATC ATCAGGAGTA CGGATAAAAT GCTTGATGGT CGGGAGAGGC ATAAACTCCG 1021 TCAGCCAGTT GAGGCGGACC ATCTCATCTG TAACATCATT GGCAACGCTA CCTTTGCCAT 1081 GTTTCAGAAA CAACTCTGGC GCATCGGGCT TCCCATACAA GCGATAGATT GTCGCACCTG 1141 ATTGCCCGAC ATTATCGCGA GCCCATTTAT ACCCATATAA ATCAGCGTCC ATGTTGGAGT 1201 TTAAGCGCGG ACGGGAGCAA GACGTTTCCC GTTGAATATG GCTCATAACA CCCCTTGTAT 1261 TACTGTTTAT GTAAGCAGAC AGTTTTATTG TTCATGATGA TATATTTTTA TCTTGTGCAA 1321 TGTAACATCA GAGATTTTGA GACACAACGT GGCTTTGTTG AATAAATCGA ACTTTTGCTG 1381 AGTTGAAGGA TCAGCTATTT AGAAAAATAA ACAAATAGGT CATGACCAAA ATCCCTTAAC 1441 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 1501 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 1561 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 1621 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 1681 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 1741 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 1801 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 1861 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 1921 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 1981 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 2041 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 2101 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 2161 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 2221 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGATGAGCGC CTGATGCGGT 2281 ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAA TGGTGCACTC TCAGTACAAT 2341 CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC 2401 ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC 2461 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT 2521 TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA 2581 AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC 2641 GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC 2701 ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA 2761 GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT 2821 GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA 2881 TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG 2941 ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA 3001 ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG 3061 TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC 3121 CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC TAGTCATGCC CCGCGCCCAC 3181 CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC CCTTAAGTTA 3241 ATTAACATTA CTCGCATCCA TTCTCAGGCT GTCTCGTCTC GTCTCCGTGC TCTTCAATGA 3301 GGAGCGAGAC CAGTGTTCTA TGTCCCCATC GTCAGTCATG GTAGCCGCTT CAATAGGGAT 3361 AACAGGGTAA TCCATGCCAT ATGACGAGAT GGCGCTGAGG TGTCAATCGT CGGAGCCGCT 3421 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 3481 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 3541 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 3601 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 3661 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 3721 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 3781 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 3841 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 3901 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 3961 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 4021 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 4081 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 4141 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG 4201 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 4261 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 4321 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 4381 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 4441 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG 4501 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 4561 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 4621 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 4681 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 4741 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 4801 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 4861 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 4921 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 4981 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 5041 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 5101 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 5161 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 5221 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 5281 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG 5341 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAGAACGATG 5401 ATGCTCACTC TCGGGTCTCA //