LOCUS       BBF10K_003378           2412 bp    DNA     circular     17-Jun-2025
ACCESSION   BBF10K_003378
VERSION     BBF10K_003378.1  
FEATURES             Location/Qualifiers
     promoter        54..72
                     /label=T7 promoter
     promoter        5..72
                     /label=AB_T7
     CDS             complement(1225..2084)
                     /label=AmpR
     promoter        complement(2086..2189)
                     /label=AmpR promoter
     primer_bind     2208..2224
                     /label=M13 fwd
     primer_bind     complement(363..378)
                     /label=M13 rev
     rep_origin      complement(446..1033)
                     /label=ColE1
     primer_bind     280..298
                     /label=SLiCE-for
     primer_bind     complement(2252..2269)
                     /label=SLiCE-rev
     misc_feature    1037..1226
                     /label=spacer
     CDS             397..447
                     /label=spacer
     terminator      313..360
                     /label=T7 terminator
     terminator      2225..2256
                     /label=tonB terminator
     misc_feature    275..2275
                     /label=pOpen_v3 backbone
     misc_feature    2406..2411
                     /label=BsaI_site
     misc_feature    complement(78..83)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GGAG
     misc_feature    73..76
                     /label=TACT
ORIGIN
        1 GGAGTCGTCA CTAGAGGGCC CTGCATTAGG ATCGATCTCG ATCCCGCGAA ATTAATACGA
       61 CTCACTATAG GGTACTCGAG ACCATCAGGT GGACTAACAT GAATCTTTAC TCCCAAGCGA
      121 AAACGGGTGC GTGGACTAGC GAGGAGCAAA CGAAAATTCT TGGCCTGCTT GGTGTCTCGT
      181 ATTCCTCTTA GAGAGGTTAC AATAAGGGCT CCGTATGCCG AGATCGAGGG CGCACTAGGG
      241 ATAACAGGGT AATGGACCCT ACGCATCCTA GTTGCGCTGA GGTGTCAATC GTCGGAGCCG
      301 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
      361 ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG
      421 AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
      481 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
      541 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
      601 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
      661 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
      721 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
      781 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
      841 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
      901 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
      961 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     1021 AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG
     1081 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
     1141 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
     1201 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
     1261 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
     1321 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC
     1381 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
     1441 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
     1501 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
     1561 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
     1621 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
     1681 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
     1741 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
     1801 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
     1861 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
     1921 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
     1981 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
     2041 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
     2101 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
     2161 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC
     2221 AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT GGGAGAACGA
     2281 TGATGCTCAC TCTCGCTGGG CAATACGAGG TAAAGCCAGT CACCCAGTGT CGATCAACAC
     2341 CTAACCTAAC GGTAAGAGGC TCACATAATG GCACTGTCGG CGTCCCCAGG GTATTTTACG
     2401 TTAGCGGTCT CA
//