LOCUS       BBF10K_003380           2415 bp    DNA     circular     29-Apr-2025
ACCESSION   BBF10K_003380
VERSION     BBF10K_003380.1  
FEATURES             Location/Qualifiers
     promoter        23..51
                     /label=tac promoter
     promoter        5..75
                     /label=AB_pTac
     CDS             complement(1228..2087)
                     /label=AmpR
     promoter        complement(2089..2192)
                     /label=AmpR promoter
     primer_bind     2211..2227
                     /label=M13 fwd
     primer_bind     complement(366..381)
                     /label=M13 rev
     rep_origin      complement(449..1036)
                     /label=ColE1
     primer_bind     283..301
                     /label=SLiCE-for
     primer_bind     complement(2255..2272)
                     /label=SLiCE-rev
     misc_feature    1040..1229
                     /label=spacer
     CDS             400..450
                     /label=spacer
     terminator      316..363
                     /label=T7 terminator
     terminator      2228..2259
                     /label=tonB terminator
     misc_feature    278..2278
                     /label=pOpen_v3 backbone
     misc_feature    2409..2414
                     /label=BsaI_site
     misc_feature    complement(81..86)
                     /label=BsaI_site
     misc_feature    1..4
                     /label=GGAG
     misc_feature    76..79
                     /label=TACT
ORIGIN
        1 GGAGAACGAT CGTTGGCTGT GTTGACAATT AATCATCGGC TCGTATAATG TGTGGAATTG
       61 TGAGCGCTCA CAATTTACTC GAGACCCCAC ATCGTCTCTT CTGCGGATGA CTTAACACGC
      121 TAGGGAGGTG GAGTCGATTC CATCGATGGT TATAAATCAA AAAATCGGAA CGCTGTCTGG
      181 AGGATGAATC TAACGGAGGC TCGATACAGT TTCACGTTAG CGGACGGGGA GAGTGTCTTA
      241 GGGATAACAG GGTAATCAGA GGTCGGCAGT CCAGTAACGC TGAGGTGTCA ATCGTCGGAG
      301 CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT
      361 TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC
      421 GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
      481 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
      541 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
      601 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
      661 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
      721 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
      781 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
      841 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
      901 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
      961 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     1021 AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA
     1081 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
     1141 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
     1201 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT
     1261 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA
     1321 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT
     1381 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG
     1441 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA
     1501 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT
     1561 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA
     1621 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA
     1681 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA
     1741 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT
     1801 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG
     1861 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC
     1921 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT
     1981 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA
     2041 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT
     2101 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT
     2161 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG
     2221 GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAGAA
     2281 CGATGATGCT CACTCTCGTG CGTATCTCGA TCGCTCAGTC GCTTTTCGTA CTGCGCGAAA
     2341 GTTCGCACCG CTCATACACT TGGTTCCGAA GCCTGTCCTG ATATATGAAT CCAAACTAGA
     2401 GCGGGGCTGG TCTCA
//